MEM Essential & Non-Essential Amino Acids Solution (50X) is indeed playing a substantial role in cell culture. Basically, Essential amino acids cannot be synthesized In Vivo,\u00a0<\/em> thus it is extremely important to supplement the media with those to maintain proper cellular growth and expected viability. On the other hand, mammalian cells can synthesize non-essential amino acids (NEAA) through glutaminolysis, glycolysis, or via TCA cycle.<\/p>\n During low concentration of required amino acids in media, cells need to consume more glucose and glutamine, which results in an unexpected amount of biodegradation products, this , indeed acts as a hindrance in proper cellular growth and maintenance. So, adding supplements of amino acids to media reduces biosynthetic burden on cell in vitro, stimulates growth and prolongs the viability of cells.<\/p>\n Moreover, you can find the formulation for PurMa\u2122 MEM Essential Amino Acids: Formulation<\/a><\/strong><\/span><\/p>\n additionally, you can find the formulation for PurMa\u2122 MEM Non-Essential Amino Acids:Formulation<\/span><\/a><\/strong><\/p>\n PurMa Biologics manufactures the essential and non-essential amino acids using our highest quality water, PurMaTM<\/sup> Ultra-Pure Cell Culture Suited water (Cat#: P3W010101). For more information, click here: PurMa\u2122 Ultra-Pure Cell Culture Suited water<\/span><\/strong><\/a><\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" MEM Essential & Non-Essential Amino Acids Solution (50X) are indeed playing a substantial role in cell culture. Essential amino acids cannot be synthesized in vivo,\u00a0 thus it is extremely important to supplement the media with. On the other hands, mammalian cells can synthesize non-essential amino acids (NEAA) through glutaminolysis, glycolysis, or via TCA cycle. PurMa Biologics Manufactures both MEM Essential & Non-Essential Amino Acids Solution (50X)<\/p>\n","protected":false},"featured_media":127247,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[611],"product_tag":[581,613,527,614],"class_list":["post-59360","product","type-product","status-publish","has-post-thumbnail","product_cat-other-cell-and-tissue-culture-reagents","product_tag-cell-culture","product_tag-essential-amino-acids","product_tag-mem","product_tag-non-essential-amino-acids","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/59360","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=59360"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127247"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=59360"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=59360"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=59360"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=59360"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Special Features<\/h2>\n
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References<\/h2>\n
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\n General specifications<\/strong><\/td>\n<\/tr>\n \n Parameter<\/td>\n Specification<\/td>\n<\/tr>\n \n Grade<\/td>\n Cell Culture Suited<\/td>\n<\/tr>\n \n Appearance<\/td>\n Clear<\/td>\n<\/tr>\n \n pH<\/td>\n 7.4 \u00b1 0.1<\/td>\n<\/tr>\n \n Osmolality<\/td>\n 275-360 mOsm\/L<\/td>\n<\/tr>\n \n Filtration<\/td>\n Sterile filtered using 0.2 \u00b5m filter<\/td>\n<\/tr>\n \n Indicator<\/td>\n NA<\/td>\n<\/tr>\n \n Conductivity of Used Water<\/td>\n 0.056 \u00b5s\/cm2<\/sup><\/td>\n<\/tr>\n \n Resistancy of Used Water<\/td>\n 18.2 \u2126m<\/td>\n<\/tr>\n \n Shipping Condition<\/td>\n Dry Ice<\/td>\n<\/tr>\n \n Refractive index<\/td>\n n<\/em>20\/D 1.34 (lit.)<\/td>\n<\/tr>\n \n Molecular Biology Parameters<\/strong><\/td>\n<\/tr>\n \n DNases & RNases<\/td>\n NOT Detected<\/td>\n<\/tr>\n \n proteases<\/td>\n NOT Detected<\/td>\n<\/tr>\n \n phosphatases<\/td>\n NOT Detected<\/td>\n<\/tr>\n \n Pathogens Evaluation<\/strong><\/td>\n<\/tr>\n \n Endotoxin<\/td>\n NMT< 2EU\/mL<\/td>\n<\/tr>\n \n Mycoplasma<\/td>\n Negative<\/td>\n<\/tr>\n \n Mold & Fungi\/Yeast<\/td>\n Negative<\/td>\n<\/tr>\n \n Bacteria<\/td>\n Negative<\/td>\n<\/tr>\n \n Sodium pyruvate<\/td>\n NA<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n