{"id":59400,"date":"2023-07-26T19:11:30","date_gmt":"2023-07-26T19:11:30","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=59400"},"modified":"2026-07-31T15:47:40","modified_gmt":"2026-07-31T21:47:40","slug":"mycoplasma-pcr-detection-kit","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mycoplasma-pcr-detection-kit\/","title":{"rendered":"Mycoplasma PCR Detection Kit"},"content":{"rendered":"<h2>Background<\/h2>\n<p>Undoubtedly, PurMa Biologics is the pioneering company in both mycoplasma detection and elimination reagents. Moreover, PurMa<sup>\u2122 <\/sup>Mycoplasma PCR Detection Kit is a fast, easy to use, highly accurate kit. The kit contains a 2X green master mix and needs just 1-2\u00b5l of your sample to be tested.<\/p>\n<h2>PurMa<sup>\u2122 <\/sup>Mycoplasma PCR Detection Kit (50 Reactions) Cat # <u>P5M011404-50<\/u> Contents<\/h2>\n<ul>\n<li>PurMa<sup>TM<\/sup> PCR 2X Mix (containing green dye, no need for loading dye\u00a0 includes all the primers) (700 \u00b5l)<\/li>\n<li>It also contains reaction buffer (2500\u00b5l)<\/li>\n<li>More importantly, Positive control. The lysed mycoplasma which does not carry any live mycoplasma (50ul)<\/li>\n<li>Negative Control. Lysed COS1 cells serves as the negative control (mycoplasma free), ready to proceed to the PCR and (50\u00b5l).<\/li>\n<li>Lastly, PurMa\u2122 Ultra-Pure Nuclease Free Water (Cat# P3W013104) (1ml)<\/li>\n<\/ul>\n<p>To gain the best results, please strictly follow the guidance: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/07\/Mycoplasma-Detection-Kit_User-Manual.pdf\">Protocol<\/a><\/strong><\/p>\n<h2>Mycoplasma Contamination<\/h2>\n<p>Contamination with mycoplasma does not cause turbidity or obvious changes in cell culture media, and therefore it is overlooked in many laboratories.<\/p>\n<p>Although, to date, more than 190 mycoplasma species have been reported, it is eight species that account for more than 95% of all mycoplasma contamination in cell culture, including: <em>M. arginini<\/em> (bovine), <em>M. fermentans<\/em> (human), <em>M. hominis<\/em> (human), <em>M. hyorhinis<\/em> (porcine), <em>M. orale<\/em> (human), <em>M. pirum<\/em> (human), <em>M. salivarium<\/em> (human), and <em>Acholeplasma laidlawii<\/em> (bovine).<\/p>\n<p>Our sophisticated PurMa<sup>\u2122 <\/sup>Mycoplasma PCR Detection Kit not only detects the mycoplasma in your cell culture system, but also determines the exact mycoplasma strain that is the cause of contamination.<\/p>\n<h2>Consequences of contamination with mycoplasma<\/h2>\n<ol>\n<li>Gradual deterioration of the cell line, which is especially significant in stable cell lines (15-35%)<\/li>\n<li>Inconsistency among the results and much higher standard deviations in your statistical analysis.<\/li>\n<li>Altering DNA, RNA, and protein synthesis.<\/li>\n<li>Diminishing amino acid and ATP levels.<\/li>\n<li>Chromosomal alterations.<\/li>\n<li>Modifying host-cell immune system.<\/li>\n<\/ol>\n<h2>Mycoplasma contamination can happen in various ways<\/h2>\n<ol>\n<li>Faulty laboratory equipment. (For Instance: a dysfunctioning laminar flow could let mycoplasma-containing dust in the biosafety cabinet, thereby causing contamination of all of the media and cells within.)<\/li>\n<li>Inadequate culturing techniques, human breath contains several major mycoplasma including M. fermentans, which account for the majority of cell culture contamination (45%). Wearing a mask is absolutely needed while working in a cell culture lab, especially when checking cells under microscope.<\/li>\n<li>Getting a cell line from another laboratory may introduce Mycoplasma contamination.<\/li>\n<\/ol>\n<h2>Mycoplasma can affect the phenotypic and functional characteristics of cells <em>In Vitro<\/em><\/h2>\n<p>Including:<\/p>\n<ul>\n<li>Chromosomal aberrations<\/li>\n<li>Disruption of nucleic acid synthesis<\/li>\n<li>Changes in membrane antigenicity<\/li>\n<li>Inhibition of cell proliferation and metabolism<\/li>\n<li>Decreased transfection rates<\/li>\n<li>Changes in gene expression profiles<\/li>\n<li>Affects virus production.<\/li>\n<li>Cell death<\/li>\n<\/ul>\n<h2>Special Features<\/h2>\n<ul>\n<li>PurMa<sup>TM <\/sup>Mycoplasma PCR Detection Kit is Easy to Use<\/li>\n<li>The procedure is fast and is completed within 2 hrs.<\/li>\n<li>Our kit has high detection ability.<\/li>\n<li>Does not detect bacterial or animal sequences of DNA.<\/li>\n<li>PurMa<sup>TM <\/sup>Mycoplasma PCR Detection Kit detects the following species:\u00a0 A. Laidlawii, M. Arginine, M. Boris, M. Fermentans, M. Hominis, M. Hyorhinis and M. Orale<\/li>\n<li>PurMa<sup>TM <\/sup>Mycoplasma Detection Kit detects a 230 bp amplicon.<\/li>\n<li>The kit contains a 2X ready master mix containing all the primers and green dye (you would not need to add loading dye after amplification to run the result on agarose gel) and needs only 1-2\u00b5l of your sample to be tested.<\/li>\n<\/ul>\n<h2>References<\/h2>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/1694167\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Mycoplasma detection in cell culture by concomitant use of bisbenzamide and fluoresceinated antibody<\/span>. <\/a>Freiberg et al. In Vitro Cell Dev Biol. 1990 Jun;26(6):585-8.<\/li>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/8118618\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Mycoplasma detection by PCR analysis<\/span>. <\/a>Hopert et al. In Vitro Cell Dev Biol Anim. 1993 Oct;29A(10):819-21.<\/li>\n<\/ol>\n<table width=\"460\">\n<tbody>\n<tr>\n<td width=\"201\"><strong>Parameter<\/strong><\/td>\n<td width=\"259\"><strong>Specification<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Appearance<\/td>\n<td width=\"259\">Clear liquid\/Green<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">pH<\/td>\n<td width=\"259\">7.4 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Osmolality<\/td>\n<td width=\"259\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Endotoxin<\/td>\n<td width=\"259\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mycoplasma<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Suitability<\/td>\n<td width=\"259\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Indicator<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Sterility Tested<\/td>\n<td width=\"259\">Sterile filtered using 0.1 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Form<\/td>\n<td width=\"259\">Liquid<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Shipping Condition<\/td>\n<td width=\"259\">Dry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Undoubtedly, PurMa Biologics is the pioneering company in both mycoplasma detection and elimination reagents. Moreover, PurMa<sup>\u2122 <\/sup>Mycoplasma PCR Detection Kit is fast, easy to use, highly accurate kit. Additionally, the kit contains a 2X green master mix .<\/p>\n","protected":false},"featured_media":127245,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[12],"product_tag":[571,572,573],"class_list":["post-59400","product","type-product","status-publish","has-post-thumbnail","product_cat-mycoplasma-detection-and-elimination-reagents","product_tag-mycoplasma","product_tag-mycoplasma-pcr","product_tag-pcr-detection-kit","entry","has-media","first","instock","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/59400","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=59400"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127245"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=59400"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=59400"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=59400"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=59400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}