{"id":59420,"date":"2023-07-27T16:23:50","date_gmt":"2023-07-27T16:23:50","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=59420"},"modified":"2026-07-31T15:47:40","modified_gmt":"2026-07-31T21:47:40","slug":"mycoplasma-growth-media","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mycoplasma-growth-media\/","title":{"rendered":"Mycoplasma Growth Media"},"content":{"rendered":"<h2>PurMa\u2122 Mycoplasma Growth Media<\/h2>\n<p>PurMa\u2122 Mycoplasma Growth Media is a proprietary combination which provides an excellent environment for various species of mycoplasma to grow.<\/p>\n<h2>Nutrition<\/h2>\n<p>Mycoplasma has a complex nutritional requirement. This therefore, makes them dependent on external supplies. PurMa\u2122 Mycoplasma Growth Media is ideal for growing different strains. Mycoplasmas have three important features: they are categorized as Mollicutes class, they also possess low GC content, and they basically don\u2019t have a cell wall. Additionally, mycoplasma has a simplified metabolic pathway and therefore it is hard to grow this organism, especially under laboratory conditions.<\/p>\n<h2>Mycoplasma Detection<\/h2>\n<p>PurMa\u2122 Mycoplasma Growth Media is also used for detecting the exact strain of contaminating mycoplasma.<\/p>\n<p>The best tool for evaluating \u00a0the presence of mycoplasma in any liquid such as media is by using the PCR method. The mycoplasma agar can also be used by growing mycoplasma and counting mycoplasma fried egg\u201d shape colonies.<\/p>\n<ul>\n<li>Initially, \u00a0Incubate the contaminated samples of media, FBS or cell line in 5 ml of PurMa\u2122 MycoGrowth media (liquid). \u00a0The condition must be anaerobic while shaking.<\/li>\n<li>Pipet 100 \u00b5l of the media on an PurMa\u2122 MycoAgar plate.<\/li>\n<li>After three days individual fried egg structure will start to show up.<\/li>\n<li>Pick up several colonies and grow them in PurMa\u2122 MycoGrowth liquid media.<\/li>\n<li>Use PurMa\u2122 Mycoplasma PCR Detection kit (Cat#: P5M011404) to amplify the fragment.<\/li>\n<li>Have the PCR reaction cleaned and sent for sequencing.<\/li>\n<li>The 230bp PCR fragment resulted from our kit will clearly determine the strain of your contaminant.<\/li>\n<\/ul>\n<h2>Special Features<\/h2>\n<ul>\n<li>Easy-to-Use<\/li>\n<li>Is an excellent tool to determine your exact mycoplasma contamination.<\/li>\n<li>Contains a proprietary formulation which maximizes the growth of all known mycoplasmas.<\/li>\n<li>Rigorously tested for bacteria, endotoxin, and mold.<\/li>\n<li>Free of all mycoplasmas inhibiting growth elements.<\/li>\n<\/ul>\n<h2>References<\/h2>\n<ol>\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/14197875\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII<\/span>. <\/a>TOURTELLOTTE et al. J Bacteriol. 1964 Jul;88(1):11-5.<\/li>\n<li><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/5773008\/\" rel=\"nofollow noopener\" target=\"_blank\">Comparison of undefined medium and its dialyzable fraction for growth of Mycoplasma. <\/a><\/span>Pollock et al. 1969 Feb;97(2):522-5.<\/li>\n<\/ol>\n<table width=\"460\">\n<tbody>\n<tr>\n<td width=\"201\"><strong>Parameter<\/strong><\/td>\n<td width=\"259\"><strong>Specification<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Appearance<\/td>\n<td width=\"259\">Liquid\/Powder<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">pH<\/td>\n<td width=\"259\">7.4 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Osmolality<\/td>\n<td width=\"259\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Endotoxin<\/td>\n<td width=\"259\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Mycoplasma<\/td>\n<td width=\"259\">Negative<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Suitability<\/td>\n<td width=\"259\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Indicator<\/td>\n<td width=\"259\">NA<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Sterility Tested<\/td>\n<td width=\"259\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Form<\/td>\n<td width=\"259\">Liquid\/ powder<\/td>\n<\/tr>\n<tr>\n<td width=\"201\">Shipping Condition<\/td>\n<td width=\"259\">Ambient<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Mycoplasma Growth Media is a proprietary combination. This media does not only provide an excellent environment for various species of mycoplasma to grow, but is also used for detecting the exact strain of contaminating mycoplasma.<\/p>\n","protected":false},"featured_media":127253,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[12],"product_tag":[575,571,576,577],"class_list":["post-59420","product","type-product","status-publish","has-post-thumbnail","product_cat-mycoplasma-detection-and-elimination-reagents","product_tag-agar","product_tag-mycoplasma","product_tag-mycoplasma-growth","product_tag-mycoplasma-growth-media","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/59420","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=59420"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/127253"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=59420"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=59420"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=59420"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=59420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}