{"id":59424,"date":"2023-07-27T16:27:08","date_gmt":"2023-07-27T16:27:08","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&p=59424"},"modified":"2026-07-31T15:47:40","modified_gmt":"2026-07-31T21:47:40","slug":"mycoplasma-treatment-kit","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/mycoplasma-treatment-kit\/","title":{"rendered":"Mycoplasma Treatment Kit (MTK) (100X)"},"content":{"rendered":"

PurMa\u2122\u00a0<\/sup>Mycoplasma Treatment Kit (MTK) (100X) is a valuable tool for eliminating mycoplasma from live cells.<\/p>\n

Important Note<\/h2>\n

Completing the eradication of mycoplasma from a contaminated cell line is laborious, time consuming as well as expensive. The procedure might also cause more contamination. So, we strongly recommend disposing of the contaminated cell line and starting a new culture if possible. This, however, might not be possible due to high value cell lines such as monoclonal antibodies or stable cell lines.<\/p>\n

Feeder Cells<\/h2>\n

During the eradication process, fresh spleen cells of a healthy mouse are needed. Before starting the process:<\/p>\n

Isolate the spleen cells from a healthy adult mouse.<\/p>\n

Resuspend in 10 ml media of choice containing 20% FBS in a 15ml snap tube and place in 37\u00b0C CO2<\/sub> incubator.<\/p>\n

For detailed procedure regarding the isolating spleen cells, click here: Detailed Procedure<\/span><\/a><\/strong><\/p>\n

before you start<\/h2>\n
    \n
  1. Decontaminate your CO2 incubator, Hood, and benches before starting the procedure, otherwise in no time, the contamination will be back into your cultured cells.<\/li>\n
  2. Use unopened media bottles and fresh FBS.<\/li>\n
  3. During the following mentioned procedure and after that, wear mask at all times while you work in cell culture lab.<\/li>\n
  4. Spray your gloves, bench, hood with PurMa\u2122 Mycospray (1X) (Cat#: P5M010405) before and after work. PurMaTM<\/sup> Mycospray (1X) instantly eliminates\/ deactivates mycoplasma.<\/li>\n<\/ol>\n

    For detailed protocol of mycoplasma elimination, Click here: PurMa\u2122 Complete Mycoplasma Removal for Cell Culture<\/a><\/strong><\/span><\/p>\n

    References<\/strong><\/h2>\n
      \n
    1. Eradication of mycoplasma contaminations. <\/a><\/span>Uphoff et al. Methods Mol Biol. 2013;946:15-26.<\/li>\n
    2. The elimination of mycoplasma from infected hybridomas by passaging in BALB\/c mice. <\/a><\/span>Carroll et al. J Immunol Methods. 1988 Apr 6;108(1-2):189-93.<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
      Parameter<\/strong><\/td>\nSpecification<\/strong><\/td>\n<\/tr>\n
      Appearance<\/td>\nRed liquid<\/td>\n<\/tr>\n
      pH<\/td>\n7.4 \u00b1 0.1<\/td>\n<\/tr>\n
      Osmolality<\/td>\n275-360 mOsm\/L<\/td>\n<\/tr>\n
      Endotoxin<\/td>\nNMT< 2EU\/mL<\/td>\n<\/tr>\n
      Mycoplasma<\/td>\nNegative<\/td>\n<\/tr>\n
      Suitability<\/td>\n\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n
      Indicator<\/td>\nNA<\/td>\n<\/tr>\n
      Sterility Tested<\/td>\nSterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n
      Form<\/td>\nLiquid<\/td>\n<\/tr>\n
      Shipping Condition<\/td>\nDry Ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

      \n","protected":false},"excerpt":{"rendered":"

      PurMa\u2122\u00a0<\/sup>Mycoplasma Treatment Kit (MTK) (100X) is a valuable tool for eliminating mycoplasma from live cells. PurMa\u2122 MTRI, PurMa\u2122 MTRII as well as PurMa\u2122 MTRIII<\/p>\n

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