{"id":98562,"date":"2024-07-04T08:17:29","date_gmt":"2024-07-04T14:17:29","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=98562"},"modified":"2026-09-24T11:41:05","modified_gmt":"2026-09-24T17:41:05","slug":"buy-dmem-f-12-medium-purma-biologics-dmem-f-12-medium","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/buy-dmem-f-12-medium-purma-biologics-dmem-f-12-medium\/","title":{"rendered":"PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12)"},"content":{"rendered":"<p data-rm-block-id=\"block-1\"><strong>PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12)<\/strong><\/p>\n<h2 data-rm-block-id=\"block-2\">Background<\/h2>\n<p data-rm-block-id=\"block-3\">PurMa\u2122 Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12)\u00a0 is an enriched DMEM\/F\u201112 formulation developed for reliable, reproducible mammalian cell culture and optimized for cell types that benefit from additional nutrients and buffering. This advanced DMEM F\u201112 supports MDCK, glial cells, fibroblasts, human endothelial cells and rat fibroblasts, providing consistent performance in research and production workflows.<\/p>\n<h2 data-rm-block-id=\"block-4\">Special Features<\/h2>\n<ul>\n<li data-rm-block-id=\"block-5\">L\u2011Glutamine: 2.5 mM (stabilized where applicable)<\/li>\n<li data-rm-block-id=\"block-6\">Sodium pyruvate: 0.5 mM<\/li>\n<li data-rm-block-id=\"block-7\">Sodium bicarbonate (NaHCO3): 30 mM (2.438 g\/L) for robust CO2 buffering<\/li>\n<li data-rm-block-id=\"block-8\">High glucose level for energy\u2011intensive cell types<\/li>\n<li data-rm-block-id=\"block-9\">Non\u2011essential amino acids to support protein synthesis and growth<\/li>\n<li data-rm-block-id=\"block-10\">Phenol red pH indicator for culture monitoring<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-11\">Why choose PurMa Advanced DMEM F\u201112 (DMEM F\u201112 formulation advantages)<\/h2>\n<ul>\n<li data-rm-block-id=\"block-12\">Consistency: Manufactured under stringent QC for lot\u2011to\u2011lot uniformity.<\/li>\n<li data-rm-block-id=\"block-13\">Moreover, enriched composition of advanced DMEM F\u201112: Compared with standard DMEM, DMEM F\u201112 provides additional pyruvate, amino acids and optimized buffering. See \u201cDMEM vs DMEM\/F-12\u201d for differences in nutrient profile and application.<\/li>\n<li data-rm-block-id=\"block-14\">Versatility: Well\u2011suited for a broad range of adherent and suspension mammalian cell lines that demand enriched media.<\/li>\n<li data-rm-block-id=\"block-15\">Ready for supplementation: Compatible with serum, defined supplements, growth factors, and antibiotics per your protocol.<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-16\">Applications<\/h2>\n<ul>\n<li data-rm-block-id=\"block-17\">Advanced Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12) is suitable for\u00a0 MDCK cell culture<\/li>\n<li data-rm-block-id=\"block-18\">Glial and neural cell maintenance<\/li>\n<li data-rm-block-id=\"block-19\">Primary and immortalized fibroblast culture<\/li>\n<li data-rm-block-id=\"block-20\">Human endothelial cell expansion<\/li>\n<li data-rm-block-id=\"block-21\">Routine cell maintenance, proliferation assays, and transfection workflows<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-22\">Handling &amp; Storage<\/h2>\n<ul>\n<li data-rm-block-id=\"block-23\">Store Advanced Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12) unopened at 2\u20138 \u00b0C protected from light.<\/li>\n<li data-rm-block-id=\"block-24\">Warm to 37 \u00b0C before use and handle aseptically.<\/li>\n<li data-rm-block-id=\"block-25\">Use prepared Advanced DMEM F\u201112\u00a0 according to your lab SOPs; supplement with serum or additives as required.<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-26\">Quality &amp; Support Dulbecco\u2019s Modified Eagle\u2019s Medium\/F-12 (DMEM F-12)\u00a0is produced in a controlled manufacturing environment with full documentation and Certificate of Analysis available for each lot. Contact technical support for media optimization and supplementation guidance.<\/p>\n<h2 data-rm-block-id=\"block-27\">\u00a0Formulation<\/h2>\n<p data-rm-block-id=\"block-28\">For complete formulation click here which is identical to thermofisher and\u00a0 gibco advanced dmemf12:<a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/DMEM-F-12-Formultaion-P3S010106.pdf\"><strong> DMEM F-12 Formulation<\/strong><\/a><\/p>\n<h2 data-rm-block-id=\"block-29\">References:<\/h2>\n<ol>\n<li data-rm-block-id=\"block-30\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/13669362\/\" rel=\"nofollow noopener\" target=\"_blank\">\u00a0Clonal growth of mammalian cells in a chemically defined, synthetic medium <\/a>.<\/span> DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 \/0042-68 22(59)90043-1. PMID: 13669362.<\/li>\n<li data-rm-block-id=\"block-31\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/13669362\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Plaque production by the polyoma virus<\/span>. <\/a>DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016\/0042-6822(59)90043-1. PMID: 1366936.<\/li>\n<\/ol>\n<div class=\"table-1\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-32\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-33\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-34\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-35\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-36\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-37\">7.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-38\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-39\">275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-40\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-41\">NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-42\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-43\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-44\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-45\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-46\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-47\">Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-48\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-49\">Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-50\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-51\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-52\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-53\">Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-54\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-55\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-56\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-57\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-hams-f-12k\/\">What Is Ham&#8217;s F-12K?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A high-performance DMEM\/F-12 formulation engineered for consistent mammalian cell culture.<\/p>\n<ul>\n<li>Enriched with 2.5 mM L-glutamine, 0.5 mM sodium pyruvate, 30 mM NaHCO3, high glucose, non-essential amino acids, and phenol red<\/li>\n<li>Ideal for MDCK, glial cells, fibroblasts, and human endothelial cultures<\/li>\n<\/ul>\n","protected":false},"featured_media":118210,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,510],"product_tag":[531,529,530],"class_list":["post-98562","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-dmem-f-12","product_tag-hams-f-12","product_tag-hams-f-12k","product_tag-kaighns","entry","has-media","first","instock","taxable","shipping-taxable","product-type-variable","has-default-attributes","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/98562","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=98562"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/118210"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=98562"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=98562"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=98562"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=98562"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}