{"id":99422,"date":"2024-07-04T19:44:43","date_gmt":"2024-07-05T01:44:43","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=99422"},"modified":"2026-08-13T13:47:15","modified_gmt":"2026-08-13T19:47:15","slug":"kaighns-hams-f-12k-medium","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/kaighns-hams-f-12k-medium\/","title":{"rendered":"PurMa\u2122 Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium"},"content":{"rendered":"<p data-rm-block-id=\"block-1\"><strong>PurMa\u2122 Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium<\/strong><\/p>\n<h2 data-rm-block-id=\"block-2\">Product Description<\/h2>\n<p data-rm-block-id=\"block-3\">PurMa\u2122 Kaighn\u2019s Modification of Ham\u2019s F\u201112 (F\u201112K) is a historically proven, enriched basal medium. This medium was originally formulated for primary cells (e.g., hepatocytes, oocytes) and is now extensively used across many mammalian cell types. Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium is formulated with components absent in older media, including putrescine, thymidine, hypoxanthine and zinc. It also contains higher levels of amino acids, vitamins, inositol, and choline to support growth in low\u2011serum or serum\u2011free conditions. It has been shown that Ham F12K is promising in rat and chicken\u00a0 primary cell culture. F-12K is a modification of Ham\u2019s F\u201112 medium.<\/p>\n<h2 data-rm-block-id=\"block-4\"><strong>Key formulation highlights<\/strong><\/h2>\n<ul>\n<li data-rm-block-id=\"block-5\">Low glucose: ~1.26 g\/L (suitable for glucose\u2011sensitive cells).<\/li>\n<li data-rm-block-id=\"block-6\">Sodium bicarbonate: standard 2.5 g\/L (alternative formulations available: 1.5 g\/L Cat# P3S057108 and 3.7 g\/L Cat# P3S032108 to match different CO2 settings).<\/li>\n<li data-rm-block-id=\"block-7\">L\u2011glutamine: 2 mM (\u2248292 mg\/L).<\/li>\n<li data-rm-block-id=\"block-8\">Unique additives: putrescine, thymidine, hypoxanthine and zinc, which support nucleotide metabolism and reduce clumping.<\/li>\n<li data-rm-block-id=\"block-9\">Elevated concentrations: amino acids, vitamins, inositol and choline for robust proliferation and viability.<\/li>\n<li data-rm-block-id=\"block-10\">Manufactured using &gt;99% pure amino acids and stringent QC for lot\u2011to\u2011lot consistency.<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-11\"><strong>Applications<\/strong><\/h2>\n<ul>\n<li data-rm-block-id=\"block-12\">Primary cells: hepatocytes, oocytes, and other sensitive primary cultures.<\/li>\n<li data-rm-block-id=\"block-13\">Cell lines: HeLa, Jurkat, MCF\u20117, PC12, PBMCs, astrocytes, hybridomas, bone marrow cultures, and other adherent\/suspension cells.<\/li>\n<li data-rm-block-id=\"block-14\">Use cases: routine maintenance, proliferation assays, transfection, low\u2011serum culture, and sensitive functional assays.<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-15\"><strong>Why choose PurMa\u2122 Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium<\/strong><\/h2>\n<ul>\n<li data-rm-block-id=\"block-16\">Decades of formulation expertise and a broad media library (1,500+ media) for application support.<\/li>\n<li data-rm-block-id=\"block-17\">High\u2011purity components increase reproducibility across labs and experiments.<\/li>\n<li data-rm-block-id=\"block-18\">Multiple NaHCO3 options to match CO2 incubator conditions and maintain pH stability.<\/li>\n<li data-rm-block-id=\"block-19\">Competitive pricing with comprehensive documentation (COA available).<\/li>\n<\/ul>\n<h2 data-rm-block-id=\"block-20\"><strong>Storage &amp; handling<\/strong><\/h2>\n<ul>\n<li data-rm-block-id=\"block-21\">Store unopened at 2\u20138 \u00b0C; protect from light.<\/li>\n<li data-rm-block-id=\"block-22\">Supplement with serum or growth factors per your protocol.<\/li>\n<li data-rm-block-id=\"block-23\">Use aseptic technique; follow your lab SOPs and run routine mycoplasma and contamination checks.<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-24\">Call to action Request a Certificate of Analysis (COA), sample, or technical support for protocol optimization.<\/p>\n<h2 data-rm-block-id=\"block-31\">FAQs<\/h2>\n<p data-rm-block-id=\"block-31\">Q1: What is Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium) medium used for?<br \/>\nA1: Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium is used for culturing primary cells (e.g., hepatocytes, oocytes) and many established cell lines. Its enriched composition reduces clumping and supports growth in low\u2011serum conditions.<\/p>\n<p data-rm-block-id=\"block-32\">Q2: What are the sodium bicarbonate options for PurMa F\u201112K?<br \/>\nA2: PurMa offers Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium with standard 2.5 g\/L NaHCO3 and alternative formulations with 1.5 g\/L (Cat# P3S057108) and 3.7 g\/L (Cat# P3S032108) to match different CO2 incubator settings.<\/p>\n<p data-rm-block-id=\"block-33\">Q3: Does F\u201112K contain glutamine?<br \/>\nA3: Yes. Standard PurMa F\u201112K contains 2 mM L\u2011glutamine (\u2248292 mg\/L). Handle and store according to lab SOPs.<\/p>\n<p data-rm-block-id=\"block-34\">Q4: Which cell types grow well in kaighn&#8217;s f-12 medium?<br \/>\nA4: Examples include hepatocytes, oocytes, HeLa, Jurkat, MCF\u20117, PC12, PBMCs, astrocytes, hybridomas, and other primary and transformed cells.<\/p>\n<p data-rm-block-id=\"block-35\">Q5: How should I store and handle PurMa kaighn&#8217;s f-12 medium?<\/p>\n<p data-rm-block-id=\"block-35\">A5: Store unopened at 2\u20138 \u00b0C, protect from light, and supplement per your protocol. Avoid repeated freeze\u2011thaw cycles and use aseptic technique.<\/p>\n<h2 data-rm-block-id=\"block-36\">Formulation<\/h2>\n<p data-rm-block-id=\"block-37\">For complete formulation click here:<a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/Hams-F-12K-Formulation-P3S010108.pdf\"><strong><span style=\"color: #0000ff;\"> Ham&#8217;s F-12K Formulation<\/span><\/strong><\/a><\/p>\n<h2 data-rm-block-id=\"block-38\">References<\/h2>\n<ol>\n<li data-rm-block-id=\"block-39\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/3297308\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium.<\/span> <\/a>Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.<\/li>\n<li data-rm-block-id=\"block-40\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/3056898\/\" rel=\"nofollow noopener\" target=\"_blank\">Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB\/c 3T3 mouse embryo fibroblasts. <\/a><\/span>Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007\/BF02620811.<\/li>\n<li data-rm-block-id=\"block-41\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/6273246\/\" rel=\"nofollow noopener\" target=\"_blank\"><span style=\"color: #0000ff;\">Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium.<\/span> <\/a>Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337\/diab.30.12.1022. PMID: 6273246<\/li>\n<\/ol>\n<div class=\"table-1\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-42\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-43\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-44\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-45\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-46\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-47\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-48\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-49\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-50\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-51\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-52\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-53\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-54\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-55\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-56\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-57\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-58\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-59\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-60\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-61\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-62\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-63\">\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-64\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-65\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-66\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-67\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p data-rm-block-id=\"block-68\">\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-hams-f-12k\/\">What Is Ham&#8217;s F-12K?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>PurMa\u2122 Kaighn&#8217;s Modification of Ham&#8217;s F-12-K Medium is a specialized cell culture medium designed to enhance the growth and maintenance of various cell types, providing essential nutrients and optimized conditions for cellular proliferation and experimentation.<\/p>\n","protected":false},"featured_media":117667,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,511],"product_tag":[531,529,530],"class_list":["post-99422","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-kaighns-modification-of-hams-f-12-medium-hams-f-12k","product_tag-hams-f-12","product_tag-hams-f-12k","product_tag-kaighns","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-default-attributes","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/99422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=99422"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/117667"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=99422"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=99422"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=99422"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=99422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}