{"id":99946,"date":"2024-07-04T21:18:51","date_gmt":"2024-07-05T03:18:51","guid":{"rendered":"https:\/\/purmabiologics.com\/blog\/?post_type=product&#038;p=99946"},"modified":"2026-07-24T15:22:43","modified_gmt":"2026-07-24T21:22:43","slug":"iscoves-modified-dulbeccos-medium-imdm","status":"publish","type":"product","link":"https:\/\/purmabiologics.com\/blog\/product\/iscoves-modified-dulbeccos-medium-imdm\/","title":{"rendered":"PurMa\u2122 Iscove&#8217;s Modified Dulbecco&#8217;s Medium (IMDM)"},"content":{"rendered":"<h2 data-rm-block-id=\"block-1\">Product Description<\/h2>\n<p data-rm-block-id=\"block-2\">PurMa\u2122 Iscove\u2019s Modified Dulbecco\u2019s Medium (IMDM) is a robust basal medium designed for rapidly proliferating and high\u2011density mammalian cell cultures. PurMa\u2122 IMDM delivers a stable pH environment with elevated bicarbonate and HEPES buffering, making it well suited for demanding cultures and procedures that require consistent pH control.<\/p>\n<p data-rm-block-id=\"block-3\">Performance and applications of PurMa\u2122 Iscove&#8217;s Modified Dulbecco&#8217;s Medium (IMDM) support high\u2011density growth and frequent passaging. It is commonly used for suspension and adherent lines that require enriched nutrients and stable buffering, including Jurkat, HEK, COS\u20117, and various macrophage and immune cell models. Because IMDM cell culture media replaces ferric nitrate with potassium nitrate, it lacks free iron, which can benefit adhesive cell cultures sensitive to iron\u2011mediated oxidation.<\/p>\n<h3 data-rm-block-id=\"block-4\">Key formulation highlights<\/h3>\n<table class=\"ingredient-table\" border=\"1\" cellpadding=\"6\">\n<thead>\n<tr>\n<th data-rm-block-id=\"block-5\">Component<\/th>\n<th data-rm-block-id=\"block-6\">Typical concentration<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td data-rm-block-id=\"block-7\">NaHCO\u2083 (Sodium bicarbonate)<\/td>\n<td data-rm-block-id=\"block-8\">3.02 g\/L<\/td>\n<\/tr>\n<tr>\n<td data-rm-block-id=\"block-9\">HEPES<\/td>\n<td data-rm-block-id=\"block-10\">5.9 g\/L<\/td>\n<\/tr>\n<tr>\n<td data-rm-block-id=\"block-11\">Glucose (high)<\/td>\n<td data-rm-block-id=\"block-12\">4.5 g\/L<\/td>\n<\/tr>\n<tr>\n<td data-rm-block-id=\"block-13\">L\u2011glutamine<\/td>\n<td data-rm-block-id=\"block-14\">0.584 g\/L<\/td>\n<\/tr>\n<tr>\n<td data-rm-block-id=\"block-15\">Sodium pyruvate<\/td>\n<td data-rm-block-id=\"block-16\">110 mg\/L (1.0 mM)<\/td>\n<\/tr>\n<tr>\n<td data-rm-block-id=\"block-17\">Phenol red<\/td>\n<td data-rm-block-id=\"block-18\">15 mg\/L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h4 data-rm-block-id=\"block-20\">Benefits of PurMa\u2122 Iscove&#8217;s Modified Dulbecco&#8217;s Medium (IMDM)<\/h4>\n<ul>\n<li data-rm-block-id=\"block-21\">Consistent performance: manufactured under strict QC with Certificates of Analysis for each lot.<\/li>\n<li data-rm-block-id=\"block-22\">Enhanced stability: buffering and nutrient profile reduce culture stress during incubator disturbances.<\/li>\n<li data-rm-block-id=\"block-23\">Versatile: suitable for both suspension and adherent work at high cell densities.<\/li>\n<li data-rm-block-id=\"block-24\">Application ready: ideal for transfection protocols, hybridoma culture, immune cell expansion, and production workflows.<\/li>\n<\/ul>\n<p data-rm-block-id=\"block-25\">Handling &amp; storage Store unopened IMDM at 2\u20138 \u00b0C and protect from light. Warm to 37 \u00b0C before use and supplement per your protocol (e.g., 5\u201310% FBS or defined supplements). Avoid repeated freeze\u2013thaw cycles. Validate performance with your cell lines before large\u2011scale use.<\/p>\n<p data-rm-block-id=\"block-26\">Documentation &amp; support Complete Iscove&#8217;s Modified Dulbecco&#8217;s Medium (IMDM) formulation and Certificate of Analysis (COA) are available on our website. PurMa technical support can assist with media optimization, supplementation strategies, and protocol adaptation.<\/p>\n<h4 data-rm-block-id=\"block-27\">Complete formulation is available on our website as well as here : <span style=\"color: #0000ff;\"><strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/IMDM-Formulation-P3S010107.pdf\" target=\"_blank\" rel=\"noopener\"><span style=\"color: #0000ff;\"> IMDM Formulation<\/span><\/a>\u00a0<\/strong><\/span><\/h4>\n<h4 data-rm-block-id=\"block-28\">FAQs<\/h4>\n<p data-rm-block-id=\"block-28\">Q1: What is IMDM iscoves modified dulbeccos medium ) used for?<br \/>\nA1: IMDM (Iscove\u2019s Modified Dulbecco\u2019s Medium) is used for high\u2011density, rapidly proliferating mammalian cell cultures, including Jurkat, HEK, COS\u20117, macrophages, and hybridomas.<\/p>\n<p data-rm-block-id=\"block-29\">Q2: How does IMDM differ from DMEM?<br \/>\nA2: IMDM has an enriched vitamin and amino acid profile, higher buffering (NaHCO3 + HEPES), and uses potassium nitrate instead of ferric nitrate, improving performance for some adhesive cells.<\/p>\n<p data-rm-block-id=\"block-30\">Q3: Does PurMa IMDM contain L\u2011glutamine?<br \/>\nA3: Yes. PurMa IMDM contains 0.584 g\/L L\u2011glutamine; handle and store per lab SOPs and supplement when required.<\/p>\n<p data-rm-block-id=\"block-31\">Q4: How should I store and prepare IMDM?<br \/>\nA4: Store unopened at 2\u20138 \u00b0C, protect from light, and warm to 37 \u00b0C before use. Supplement with serum or defined additives per your protocol and avoid repeated freeze\u2013thaw cycles.<\/p>\n<p data-rm-block-id=\"block-32\">Q5: Is IMDM cell culture suited for serum\u2011free form of medium?<br \/>\nA5: IMDM can be used in serum\u2011free or defined systems with appropriate supplementation of growth factors, hormones, or serum replacements depending on your cell type.<\/p>\n<h2 data-rm-block-id=\"block-33\"><strong>References<\/strong><\/h2>\n<ol>\n<li data-rm-block-id=\"block-34\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/13669362\/\" rel=\"nofollow noopener\" target=\"_blank\">Plaque production by the polyoma virus. <\/a><\/span>DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016\/0042-6822(59)90043-1. PMID: 13669362.<\/li>\n<li data-rm-block-id=\"block-35\"><span style=\"color: #0000ff;\"><a style=\"color: #0000ff;\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC2184195\/\" rel=\"nofollow noopener\" target=\"_blank\">Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes<\/a>.<\/span> J Exp Med. 1978 Mar 1; 147(3): 923\u2013933. doi:\u00a010.1084\/jem.147.3.923 PMCID: PMC2184195<\/li>\n<\/ol>\n<div class=\"table-2\">\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\" data-rm-block-id=\"block-36\">Parameter<\/th>\n<th align=\"left\" data-rm-block-id=\"block-37\">Specification<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-38\">Appearance<\/td>\n<td align=\"left\" data-rm-block-id=\"block-39\">Red, clear liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-40\">pH<\/td>\n<td align=\"left\" data-rm-block-id=\"block-41\">\u00a07.2 \u00b1 0.1<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-42\">Osmolality<\/td>\n<td align=\"left\" data-rm-block-id=\"block-43\">\u00a0275-360 mOsm\/L<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-44\">Endotoxin<\/td>\n<td align=\"left\" data-rm-block-id=\"block-45\">\u00a0NMT&lt; 2EU\/mL<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-46\">Mycoplasma<\/td>\n<td align=\"left\" data-rm-block-id=\"block-47\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-48\">Suitability<\/td>\n<td align=\"left\" data-rm-block-id=\"block-49\">\u00a0Suitable for mammalian cell culture<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-50\">Additive<\/td>\n<td align=\"left\" data-rm-block-id=\"block-51\">\u00a0Sodium pyruvate<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-52\">Indicator<\/td>\n<td align=\"left\" data-rm-block-id=\"block-53\">\u00a0Phenol red<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-54\">Mycoplasma Detection<\/td>\n<td align=\"left\" data-rm-block-id=\"block-55\">Negative<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-56\">Sterility Tested<\/td>\n<td align=\"left\" data-rm-block-id=\"block-57\">\u00a0Sterile filtered using 0.22 \u00b5m filter<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-58\">Form<\/td>\n<td align=\"left\" data-rm-block-id=\"block-59\">Liquid<\/td>\n<\/tr>\n<tr>\n<td align=\"left\" data-rm-block-id=\"block-60\">Shipping Condition<\/td>\n<td align=\"left\" data-rm-block-id=\"block-61\">\u00a0Room temperature<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<p data-rm-block-id=\"block-62\">\n<p><strong>Learn more:<\/strong> <a href=\"\/what-is-imdm\/\">What Is IMDM?<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A high-buffer, high-glucose basal medium optimized for high-density, fast-growing cell lines.<\/p>\n<ul>\n<li>Suited to demanding lines such as Jurkat, HEK, COS-7, and macrophages<\/li>\n<li>High buffering capacity and high glucose for fast growth<\/li>\n<\/ul>\n","protected":false},"featured_media":117671,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[283,597],"product_tag":[581,671,680,681,545,594],"class_list":["post-99946","product","type-product","status-publish","has-post-thumbnail","product_cat-cell-culture-media","product_cat-iscoves-modified-dulbeccos-medium-imdm","product_tag-cell-culture","product_tag-dulbeccos","product_tag-imdm","product_tag-iscoves","product_tag-phenol-red","product_tag-standard","entry","has-media","first","instock","sale","taxable","shipping-taxable","purchasable","product-type-variable","has-default-attributes","has-product-nav","col","span_1_of_4","owp-content-center","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product\/99946","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/comments?post=99946"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media\/117671"}],"wp:attachment":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/media?parent=99946"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_brand?post=99946"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat?post=99946"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_tag?post=99946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}