{"id":510,"count":3,"description":"<h1>DMEM-F-12<\/h1>\r\n(Dulbecco\u2019s Modified Eagle Medium Nutrient-Mixture F-12) provides superior quality, consistency, and great control in mammalian cell culture.\r\n\r\nThis formulation combines DMEM\u2019s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with DMEM-F-12 \u2019s wide variety of components.\r\n\r\nThis media is better than DMEM and advanced IMDM on MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. It contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).\r\n\r\nIn general, DMEM- F-12 is more enriched but contains the following major changes compared to DMEM:\r\n<ul>\r\n \t<li>2.5 mM L-glutamine,<\/li>\r\n \t<li>0.5 mM sodium pyruvate,<\/li>\r\n \t<li>30mM NaHCO3 (2.438g\/L)\r\nHigh glucose,<\/li>\r\n \t<li>Non-Essential Amino Acids,<\/li>\r\n \t<li>Sodium pyruvate,<\/li>\r\n \t<li>Phenol Red<\/li>\r\n \t<li>L-glutamine<\/li>\r\n<\/ul>\r\nIn some formulations,\u00a0the concentration of sodium bicarbonate used is lower than DMEM, therefore requires a 5\u201310% CO2 environment to maintain physiological pH.","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/dmem-f-12\/","name":"DMEM F-12","slug":"dmem-f-12","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/510","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=510"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}