{"id":588,"count":4,"description":"

DMSO-Free Cryoprotective Media<\/h1>\nPurMa Biologics Manufactures Cryoprotective Media without DMSO or other penetrating agent. To emphasize, DMSO-free cryoprotective needs to apply only for certain cell lines which are damaged or poorly performing in presence of DMSO or other penetrating agents.\r\n\r\nlimitations and disadvantages of DMSO<\/strong>\r\n
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  1. Basically, it has a broad toxicity.<\/li>\r\n \t
  2. Additionally, it modifies the expression of cells such an NK and T cells<\/li>\r\n \t
  3. It alters the mRNA level of the de novo<\/em> DNA methyltransferase DNMT3A. this therefore, accompanies by hyper- or hypo-methylation of many genetic loci.<\/li>\r\n \t
  4. Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.<\/li>\r\n \t
  5. Damages the mitochondria in certain cell culture models such as astrocytes.<\/li>\r\n \t
  6. Negatively impacs cellular membrane\/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.<\/li>\r\n \t
  7. Besides, it Induces unwanted stem cell differentiation.<\/li>\r\n \t
  8. Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated sage of DMSO even at sub-toxic levels.<\/li>\r\n \t
  9. Afterwards, it is necessary to remove DMSO by intensive washing. That in turns results in cell sensitivity to osmotic\/mechanical stresses occurring. This is a costly- and time-consuming process during freezing and thawing process and moreover leads to a significant loss of cell number.<\/li>\r\n<\/ol>\r\nWe produce cryoprotective media for :\r\n
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    • Serum Free condition,\u00a0 DMSO Free<\/li>\r\n \t
    • Stem Cells ,\u00a0 DMSO Free<\/li>\r\n \t
    • Whole Tissue ; DMSO Free<\/li>\r\n \t
    • Hybridoma cells ; DMSO Free<\/li>\r\n<\/ul>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cryoprotective-media\/cryoprotective-media-dmso-free\/","name":"Cryoprotective media DMSO-Free","slug":"cryoprotective-media-dmso-free","taxonomy":"product_cat","parent":58,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/588","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/58"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=588"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}