{"id":592,"count":5,"description":"<h1>Trypsin, EDTA Free<\/h1>\r\n<h2>Background<\/h2>\r\nPurMa\u2122 Trypsin EDTA Free contains ultra-pure trypsin. This enzyme comes from the serine protease family. Trypsin, initially produced in the pancreas, and \u00a0subsequently intestine activate this enzyme. Cell culture laboratories use trypsin to dissociate adherent cells for routine cell culture.\r\n\r\nTrypsin cleaves its substrates: IGDKLIPA. We purify \u00a0trypsin \u00a099% by \u00a0HPLC method \u00a0purity form bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45\u00b0 which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 &amp; Mg+2 free for the proper function of this enzyme.\r\n\r\nsulfation of Tyr154 further modulates the activity of this enzyme. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please see: <a href=\"https:\/\/purmabiologics.com\/blog\/product\/ultra-pure-cell-culture-suited-water\/\">PurMa\u2122 Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) <\/a>\r\n\r\nAdditionally, we include 2mM EDTA as it Chelates metals such as calcium, magnesium, lead, mercury, copper, iron\u2026\r\n\r\nTrypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Incidentally, mycoplasma slowly contaminates and deteriorates the cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.\r\n\r\nPurMa Biologics manufactures trypsin without EDTA as a complete Ready-To-Use solution that contains 1X Hanks\u2019 buffer and DOES NOT need any additional ingredients.\r\n<h2>Features<\/h2>\r\n<ul>\r\n \t<li>PurMa\u2122 Trypsin EDTA Free 0.05% does not contain calcium or magnesium ions or any other heavy metals.<\/li>\r\n \t<li>PurMa\u2122 Trypsin EDTA Free 0.05% does not contain serine protease inhibitors, including DFP, TLCK, APMSF, and AEBSEF.<\/li>\r\n \t<li>PurMa\u2122 Trypsin EDTA Free does not contain Phytates or salts of phytic acid, such as myo-inositol which is commonly present in cell culture media and inhibits trypsin activity.<\/li>\r\n \t<li>PurMa\u2122 Trypsin is rigorously evaluated for yeast, mold, mycoplasma, and endotoxin contamination.<\/li>\r\n<\/ul>\r\n<h2>Concentrations\u00a0 of Trypsin<\/h2>\r\nPurMa Biologics produces 0.05%, 0.25%, 0.5%, 2.5%, and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, we manufacture PurMa\u2122 Trypsin in Hanks\u2019 Buffer with 2mM EDTA (pH 7.8).","link":"https:\/\/purmabiologics.com\/blog\/product-category\/trypsin-edta-and-edta-free\/trypsin-edta-free\/","name":"Trypsin EDTA Free","slug":"trypsin-edta-free","taxonomy":"product_cat","parent":29,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/592","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/29"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}