{"id":601,"count":3,"description":"<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">Leibovitz\u2019s L-15 Medium<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nLeibovitz\u2019s L-15 Medium was originally designed to be used in carbon dioxide (CO<sub>2<\/sub>) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">This media contains:<\/h2>\r\n<ul>\r\n \t<li>High Level of Sodium pyruvate (5mM)<\/li>\r\n \t<li>Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.<\/li>\r\n \t<li>Basically, the buffering Capacity is maintained by other elements, mainly phosphates.<\/li>\r\n \t<li>And finally Using Galactose instead of glucose makes this media quite different.<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Application of Leibovitz\u2019s L-15<\/h2>\r\n<ul>\r\n \t<li>Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.<\/li>\r\n \t<li>Establishing human tumor cells lines such as Hep-2 cells.<\/li>\r\n \t<li>As a supplement in sterile cell media for the dissection and isolation of kidney cells.<\/li>\r\n \t<li>As an important media which provides proper buffering system for culturing of lung cells.<\/li>\r\n \t<li>A valid option for growing fish originated from cell lines.<\/li>\r\n<\/ul>\r\nPurMa Biologics manufactures 107 types of Leibovitz\u2019s L-15\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\"><strong>Why PurMa Biologics? Why we stand behind our cell culture media<\/strong><\/h2>\r\nOur products are the outcome of 30 years of experience in cutting-edge science. PurMa Biologics routinely manufactures over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We use %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\"><strong>Formulation<\/strong><\/h2>\r\nFor complete formulation please visit the \u201cformulation tab\u201d, as well as by clicking here: <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/Leibovitzs-L-15-Formulation-P3S010613.pdf\" target=\"_blank\" rel=\"noopener\"><strong>Leibovitz\u2019s L-15 Formulation<\/strong><\/a>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/leibovitzs-l-15-medium\/","name":"Leibovitz\u2019s L-15 Medium","slug":"leibovitzs-l-15-medium","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/601","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=601"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}