{"id":603,"count":3,"description":"<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">Minimum Essential Media (MEM) in Earle\u2019s Buffer<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMinimum Essential Media (MEM) in Earle\u2019s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle\u2019s salts for use in a CO2 incubator, or with Hanks\u2019 salts for use without CO2.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">PurMa\u2122 MEM media includes Earle\u2019s Buffer<\/h2>\r\nAs the composition of Earle\u2019s buffer, it contains:\r\n<ul>\r\n \t<li>Calcium chloride<\/li>\r\n \t<li>Magnesium sulfate<\/li>\r\n \t<li>Potassium chloride<\/li>\r\n \t<li>Sodium bicarbonate<\/li>\r\n \t<li>Sodium chloride<\/li>\r\n \t<li>Sodium Phosphate, monobasic<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">PurMa Biologics manufactures 118 types of MEM, it also includes:<\/h2>\r\n<ul>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>Sodium bicarbonate (2.2 g\/L)<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)<\/li>\r\n<\/ul>\r\nMEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle\u2019s buffer uses a sodium bicarbonate buffer system (2.2 g\/L) that requires a 5\u201310% CO2 environment to maintain physiological pH.","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/minimum-essential-media-mem-in-earles-buffer\/","name":"Minimum Essential Media (MEM) in Earle\u2019s Buffer","slug":"minimum-essential-media-mem-in-earles-buffer","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/603","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=603"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}