{"id":604,"count":3,"description":"<div class=\"fusion-text fusion-text-1\">\r\n<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1) certainly suits mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -\u03b1 Modification applies for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">MEM -\u03b1\u00a0 contains<\/h2>\r\n<ul>\r\n \t<li>Non-essential amino acids.<\/li>\r\n \t<li>Sodium pyruvate.<\/li>\r\n \t<li>Lipoic acid.<\/li>\r\n \t<li>Vitamin B12.<\/li>\r\n \t<li>Biotin and,<\/li>\r\n \t<li>Ascorbic acid.<\/li>\r\n \t<li>Also, MEM-\u03b1 does not contain deoxyribonucleosides and ribonucleosides.<\/li>\r\n \t<li>Moreover, PurMa<sup>TM<\/sup> MEM -\u03b1 media in Earle\u2019s buffer includes Earles\u2019 buffer<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">The composition of Earle\u2019s buffer, it contains:<\/h2>\r\n<ol>\r\n \t<li>Calcium chloride<\/li>\r\n \t<li>Magnesium sulfate<\/li>\r\n \t<li>Potassium chloride<\/li>\r\n \t<li>Sodium bicarbonate<\/li>\r\n \t<li>Sodium chloride<\/li>\r\n \t<li>Sodium Phosphate, monobasic<\/li>\r\n<\/ol>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Additionally, MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)<\/h2>\r\nit contains:\r\n<ul>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>More importantly, Sodium bicarbonate (2.2 g\/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)\u2019<\/li>\r\n \t<li>Also, PurMa Biologics manufactures 118 types of MEM-\u03b1<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Application<\/h2>\r\nThis media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, Alpha modification of Minimum Essential Medium Eagle has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast,\r\n\r\nWe stand behind our cell culture media.\r\n\r\nOur products are the outcome of 30 years of experience in cutting-edge science. PurMa Biologics manufactures over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we use %99 pure amino acids. This will significantly increase the consistency of your experiments. Considering the highest quality, our prices certainly are extremely reasonable.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Formulation<\/h2>\r\nComplete formulation is in the \u201d Formulation tab\u201d as well as here: <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-Formulation-in-Earls-Buffer-P3S310123.pdf\" target=\"_blank\" rel=\"noopener\"><strong>MEM-alpha Formulation in Earle\u2019s Buffer<\/strong><\/a>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">References<\/h2>\r\n<ol>\r\n \t<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/485260\">The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.<\/a> Keay et al.\u00a0 Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).<\/li>\r\n \t<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/1471873\">Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.<\/a> Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)<\/li>\r\n<\/ol>\r\n<\/div>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/mem-alpha-modification-in-earles-buffer-mem-%ce%b1\/","name":"MEM Alpha Modification in Earle\u2019s Buffer (MEM-\u03b1)","slug":"mem-alpha-modification-in-earles-buffer-mem-%ce%b1","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/604","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}