{"id":605,"count":3,"description":"<div class=\"fusion-text fusion-text-1\">\r\n<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">Minimum Essential Media (MEM) In Hanks\u2019 Buffer<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMinimum Essential Media (MEM) In Hanks\u2019 Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific\u00a0 requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle\u2019s buffer for use in a CO<sub>2<\/sub>\u00a0incubator, or with Hanks\u2019 salts for use without CO<sub>2<\/sub>.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">PurMa<sup>\u2122<\/sup> MEM media in Hank\u2019s buffer includes Hank\u2019s buffer<\/h2>\r\nHanks buffer, it contains:\r\n<ol>\r\n \t<li>Potassium Chloride<\/li>\r\n \t<li>Potassium Phosphate, monobasic<\/li>\r\n \t<li>Sodium Bicarbonate<\/li>\r\n \t<li>Sodium Chloride<\/li>\r\n \t<li>Sodium Phosphate, dibasic<\/li>\r\n<\/ol>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Additionally, MEM in Hanks\u2019 Buffer contains:<\/h2>\r\n<ul>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>Sodium bicarbonate (0.35 g\/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)<\/li>\r\n<\/ul>\r\nMoreover, PurMa Biologics manufactures 122 types of MEM.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Applications<\/h2>\r\nMEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Formulation<\/h2>\r\nComplete formulation click here as well as in Formulation Tab: <a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-HBSS-Formulation-in-Hanks-Buffer-P3S410122.pdf\" target=\"_blank\" rel=\"noopener\"><strong>MEM (HBSS) Formulation in Hanks\u2019 Buffer<\/strong><\/a>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">References<\/h2>\r\n<ol>\r\n \t<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/3401543\/\">Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. <\/a>Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095\/biolreprod38.4.881. PMID: 3401543<\/li>\r\n \t<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/3654371\/\">Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. <\/a>Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371<\/li>\r\n<\/ol>\r\n<\/div>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/minimum-essential-media-mem-in-hanks-buffer\/","name":"Minimum Essential Media (MEM) In Hanks\u2019 Buffer","slug":"minimum-essential-media-mem-in-hanks-buffer","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}