{"id":606,"count":3,"description":"<div class=\"fusion-text fusion-text-1\">\r\n<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">MEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. Besides, this media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.\u00a0 Furthermore, it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Ingredients<\/h2>\r\nMEM -\u03b1 has improved regular MEM as it contains:\r\n<ul>\r\n \t<li>Non-essential amino acids.<\/li>\r\n \t<li>Sodium pyruvate.<\/li>\r\n \t<li>Lipoic acid.<\/li>\r\n \t<li>Vitamin B12.<\/li>\r\n \t<li>Biotin and,<\/li>\r\n \t<li>Ascorbic acid.<\/li>\r\n \t<li>Also, MEM-\u03b1 does not contain deoxyribonucleosides and ribonucleosides.<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Moreover, PurMa\u2122 MEM -\u03b1 media in Hank\u2019s buffer includes HBSS<\/h2>\r\nComposition of HBSS buffer contains:\r\n<ul>\r\n \t<li>Potassium Chloride<\/li>\r\n \t<li>Potassium Phosphate, monobasic<\/li>\r\n \t<li>Sodium Bicarbonate<\/li>\r\n \t<li>Sodium Chloride<\/li>\r\n \t<li>Sodium Phosphate, dibasic<\/li>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>Sodium bicarbonate (0.35 g\/L)<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)<\/li>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n<\/ul>\r\nFurthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Application<\/h2>\r\nAlpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -\u03b1 Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Formulation<\/h2>\r\nadditionally, to get access to detailed formulation visit \u201d formulation Tab\u201d as well as by clicking here :<a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-Formulation-in-Hanks-Buffer-HBSS-P3S410123.pdf\" target=\"_blank\" rel=\"noopener\"><strong> MEM-alpha Formulation in Hank\u2019s Buffer (HBSS)<\/strong><\/a>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">References<\/h2>\r\n<ol>\r\n \t<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/485260\">The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.<\/a> Keay L.\u00a0 Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)<\/li>\r\n<\/ol>\r\n<\/div>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/mem-alpha-modification-mem-%ce%b1-in-hanks-buffer\/","name":"MEM Alpha Modification (MEM-\u03b1) in Hanks\u2019 Buffer","slug":"mem-alpha-modification-mem-%ce%b1-in-hanks-buffer","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/606","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=606"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}