{"id":607,"count":3,"description":"<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS)<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-\u03b1-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Ingredients<\/h2>\r\nMEM Alpha Modification with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS) is a modification of MEM.\r\n<h3 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"20\" data-lineheight=\"24px\">Moreover, MEM-\u03b1-N-EBSS contains:<\/h3>\r\n<ul>\r\n \t<li>Non-essential amino acids.<\/li>\r\n \t<li>Sodium pyruvate.<\/li>\r\n \t<li>Lipoic acid.<\/li>\r\n \t<li>Vitamin B12.<\/li>\r\n \t<li>Biotin and,<\/li>\r\n \t<li>Ascorbic acid.<\/li>\r\n<\/ul>\r\n<h3 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"20\" data-lineheight=\"24px\">Because PurMa\u2122 MEM-\u03b1-N-EBSS includes Earles\u2019 Buffer,\u00a0 it contains:<\/h3>\r\n<ol>\r\n \t<li>Calcium chloride<\/li>\r\n \t<li>Magnesium sulfate<\/li>\r\n \t<li>Potassium chloride<\/li>\r\n \t<li>Sodium bicarbonate<\/li>\r\n \t<li>Sodium chloride<\/li>\r\n \t<li>Sodium Phosphate, monobasic<\/li>\r\n<\/ol>\r\n<h3 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"20\" data-lineheight=\"24px\">PurMa\u2122 MEM -\u03b1-N-EBSS media also contains:<\/h3>\r\n<ul>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>Sodium bicarbonate (2.2 g\/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)\u2019<\/li>\r\n \t<li>PurMa Biologics manufactures 121 types of MEM -\u03b1-N-E<\/li>\r\n<\/ul>\r\n<h3 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"20\" data-lineheight=\"24px\">The nucleoside modifies this media\u00a0 from MEM-\u03b1<\/h3>\r\nit contains:\r\n<ul>\r\n \t<li>Ribonucleosides<\/li>\r\n \t<li>Deoxyribonucleosides<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Application<\/h2>\r\nMoreover, this media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-\u03b1-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Additionally, PurMa\u2122 MEM -\u03b1-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for the isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Formulation<\/h2>\r\nadditionally, you can get the complete formulation here\u201d: <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-with-Nucleosides-Formulation-in-Earls-Buffer-P3S310124.pdf\" target=\"_blank\" rel=\"noopener\">MEM alpha with Nucleoside Formulation<\/a><\/strong>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">References<\/h2>\r\n<ol>\r\n \t<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/485260\">The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium.<\/a> Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).<\/li>\r\n \t<li><a href=\"https:\/\/www.sigmaaldrich.com\/US\/en\/tech-docs\/paper\/1471873\">Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.<\/a>\u00a0 Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)<\/li>\r\n<\/ol>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/mem-alpha-with-nucleosides-in-earles-buffer-mem-%ce%b1-n-ebss\/","name":"MEM Alpha with Nucleosides in Earle\u2019s Buffer (MEM-\u03b1-N-EBSS)","slug":"mem-alpha-with-nucleosides-in-earles-buffer-mem-%ce%b1-n-ebss","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/607","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=607"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}