{"id":608,"count":3,"description":"<h1 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"34\" data-lineheight=\"47.6px\">MEM \u03b1-Modification with Nucleosides in Hanks\u2019 Buffer<\/h1>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Background<\/h2>\r\nMEM \u03b1-Modification with Nucleosides\u00a0 in Hanks\u2019 buffer provides a great nutritional culture environment during mammalian cells.\u00a0 This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-\u03b1-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Ingredients<\/h2>\r\nMEM-\u03b1 with nucleosides is basically the modified version of MEM-\u03b1 and contains:\r\n<ul>\r\n \t<li>Ribonucleosides<\/li>\r\n \t<li>Deoxyribonucleosides<\/li>\r\n \t<li>Non-essential amino acids.<\/li>\r\n \t<li>Sodium pyruvate.<\/li>\r\n \t<li>Lipoic acid.<\/li>\r\n \t<li>Vitamin B12.<\/li>\r\n \t<li>Biotin and,<\/li>\r\n \t<li>Ascorbic acid.<\/li>\r\n<\/ul>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">PurMa\u2122 MEM-\u03b1-N-HBSS contains HBSS buffer<\/h2>\r\nThe composition of HBSS buffer:\r\n<ol>\r\n \t<li>Potassium Chloride<\/li>\r\n \t<li>Potassium Phosphate, monobasic<\/li>\r\n \t<li>Sodium Bicarbonate<\/li>\r\n \t<li>Sodium Chloride<\/li>\r\n \t<li>Sodium Phosphate, dibasic<\/li>\r\n<\/ol>\r\nAdditionally, MEM-\u03b1-N-HBSS also Contains:\r\n<ul>\r\n \t<li>Low level of glucose (1.00 g\/ liter)<\/li>\r\n \t<li>Sodium bicarbonate (0.35 g\/L)<\/li>\r\n \t<li>2 mM L-glutamine (292 mg\/L)<\/li>\r\n<\/ul>\r\nPurMa Biologics manufactures 119 types of MEM-\u03b1-N-HBSS\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Application<\/h2>\r\nFurthermore,\u00a0 MEM-\u03b1-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-\u03b1-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM \u03b1-Modification is widely used for primary osteoblast cultures.\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">Formulation<\/h2>\r\nFor complete formulation\u00a0 click here as well as \u201d formulation Tab : <strong><a href=\"https:\/\/purmabiologics.com\/blog\/wp-content\/uploads\/2023\/06\/MEM-alpha-with-Nucleosides-Formulation-in-Hanks-Buffer-HBSS-P3S410124.pdf\" target=\"_blank\" rel=\"noopener\">MEM alpha with nucleoside in HBSS Buffer Formulation<\/a><\/strong>\r\n<h2 class=\"fusion-responsive-typography-calculated\" data-fontsize=\"24\" data-lineheight=\"31.2px\">References<\/h2>\r\n<ol>\r\n \t<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21835026\/\">A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. <\/a>Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186\/1756-0500-4-282. PMID: 21835026.<\/li>\r\n<\/ol>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/mem-%ce%b1-modification-with-nucleosides-in-hanks-buffer\/","name":"MEM \u03b1-Modification with Nucleosides in Hanks\u2019 Buffer","slug":"mem-%ce%b1-modification-with-nucleosides-in-hanks-buffer","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/608","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=608"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}