{"id":610,"count":3,"description":"

Basal Medium Eagle (BME) in Hanks\u2019 Buffer<\/h1>\r\n

Background<\/h2>\r\nBasal Medium Eagle (BME) in Hanks\u2019 Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle\u2019s Minimum Essential Medium (MEM) and Dulbecco\u2019s Modified Eagle\u2019s Medium (DME). PurMa Biologics presently manufactures BME with Earle\u2019s for use in a CO2<\/sub>\u00a0incubator, or with Hanks\u2019 (HBSS) salts for use without CO2<\/sub>.\r\n

Ingredients<\/h2>\r\nAs the composition, this media contains HBSS buffer\r\n\r\nHBSS buffer contains:\r\n
    \r\n \t
  1. Potassium Chloride<\/li>\r\n \t
  2. Potassium Phosphate, monobasic<\/li>\r\n \t
  3. Sodium Bicarbonate<\/li>\r\n \t
  4. Sodium Chloride<\/li>\r\n \t
  5. And lastly, Sodium Phosphate, dibasic<\/li>\r\n<\/ol>\r\n

    Application<\/h2>\r\nPurMa Biologics manufactures 117 types of BME in HBSS buffer. This media has been successfully used for a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.\r\n

    Formulation<\/h2>\r\nFor the complete formulation of Basal Medium Eagle (BME) in Hanks\u2019 Buffer click here : \u00a0BME with Hanks\u2019 Buffer (HBSS) Formulation<\/strong><\/a>\r\n

    References<\/h2>\r\n
      \r\n \t
    1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. <\/a>LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128\/jb.83.3.475-482.1962.<\/li>\r\n<\/ol>","link":"https:\/\/purmabiologics.com\/blog\/product-category\/cell-culture-media\/basal-medium-eagle-bme-in-hanks-buffer\/","name":"Basal Medium Eagle (BME) in Hanks\u2019 Buffer","slug":"basal-medium-eagle-bme-in-hanks-buffer","taxonomy":"product_cat","parent":283,"meta":[],"menu_order":0,"acf":[],"_links":{"self":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/610","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat"}],"about":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/taxonomies\/product_cat"}],"up":[{"embeddable":true,"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product_cat\/283"}],"wp:post_type":[{"href":"https:\/\/purmabiologics.com\/blog\/wp-json\/wp\/v2\/product?product_cat=610"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}