Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer

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Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer

Background

PurMa^TM Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced MEM-α. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Consequently, Advanced MEM Alpha-Modification (A-MEM-α) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α (CD-A-MEM-α) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM-alpha in Hanks' Buffer

References

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, Wun CC, Grunert GM. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Roudebush WE, Often NL, Butler WJ. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

MEM Alpha Modification (MEM-α) in Hanks’ Buffer

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MEM Alpha Modification (MEM-α) in Hanks’ Buffer

Background

MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. furthermore it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.

Ingredients

MEM -α has improved regular MEM as it contains:

Non-essential amino acids.
Sodium pyruvate.
Lipoic acid.
Vitamin B12.
Biotin and,
Ascorbic acid.
Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.

PurMa™ MEM -α media in Hank’s buffer includes HBSS

Composition of HBSS buffer contains:

Potassium Chloride
Potassium Phosphate, monobasic
Sodium Bicarbonate
Sodium Chloride
Sodium Phosphate, dibasic
Low level of glucose (1.00 g/ liter)
Sodium bicarbonate (0.35 g/L)
2 mM L-glutamine (292 mg/L)
Low level of glucose (1.00 g/ liter)

Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonabl

Application

Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Formulation

to get access to detailed formulation visit " formulation Tab" as well as by clicking here : MEM-alpha Formulation in Hank's Buffer (HBSS)

References

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer

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MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer

Background

MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

MEM-α performs better than MEM for a number of cell lines, please see the description of regular MEM-α : MEM-α

The advantages of MEM-α-MAX in HBBS over MEM-α are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Formulation

For complete formulation Click here: MEM-alpha-MAX Formulation in Hanks' Buffer (HBSS)

References

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Roudebush WE, et al. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more