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Basal Medium Eagle (BME) in Hanks’ Buffer
asal Medium Eagle (BME) in Hanks’ Buffer
Background
Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks’ (HBSS) salts for use without CO2.Ingredients
As the composition, this media contains HBSS buffer HBSS buffer contains:- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- And lastly, Sodium Phosphate, dibasic
Application
PurMa Biologics manufactures 117 types of BME in HBSS buffer. This media has been successfully used for a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.Formulation
For the complete formulation of Basal Medium Eagle (BME) in Hanks’ Buffer click here : BME with Hanks’ Buffer (HBSS) FormulationReferences
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962.
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Basal Medium Eagle (BME) in Earle’s Buffer
Basal Medium Eagle (BME) in Earle’s Buffer
Background
Basal Medium Eagle (BME) in Earle’s Buffer was originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufactures BME with Earle’s as well as in Hanks’ salts for use without CO2.Ingredients
PurMa™ BME media in Earle’s buffer contains:
Earl’s buffer includes:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- and lastly, Sodium Phosphate, monobasic
Why PurMa Biologics? Why we stand behind our cell culture media.
Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency of your experiments. Undoubtedly, considering the highest quality, our prices certainly are extremely reasonable.Application
BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.Formulation
for complete formulation click here, alternatively, you can find it in the “Formulation Tab”: BME with Earle’s Buffer Formulation References:- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
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MEM α-Modification with Nucleosides in Hanks’ Buffer
MEM α-Modification with Nucleosides in Hanks’ Buffer
Background
MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment during mammalian cells. This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.Ingredients
MEM-α with nucleosides is basically the modified version of MEM-α and contains:- Ribonucleosides
- Deoxyribonucleosides
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
PurMa™ MEM-α-N-HBSS contains HBSS buffer
The composition of HBSS buffer:- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
Application
Furthermore, MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.Formulation
For complete formulation click here as well as ” formulation Tab : MEM alpha with nucleoside in HBSS Buffer FormulationReferences
- A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
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MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)
Background
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.Ingredients
MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.Moreover, MEM-α-N-EBSS contains:
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
Because PurMa™ MEM-α-N-EBSS includes Earles’ Buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa™ MEM -α-N-EBSS media also contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)’
- PurMa Biologics manufactures 121 types of MEM -α-N-E
The nucleoside modifies this media from MEM-α
it contains:- Ribonucleosides
- Deoxyribonucleosides
Application
Moreover, this media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-α-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Additionally, PurMa™ MEM -α-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for the isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.Formulation
additionally, you can get the complete formulation here”: MEM alpha with Nucleoside FormulationReferences
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
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MEM Alpha Modification (MEM-α) in Hanks’ Buffer
MEM Alpha Modification (MEM-α) in Hanks’ Buffer
Background
MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. Besides, this media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.Ingredients
MEM -α has improved regular MEM as it contains:- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
- Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
Moreover, PurMa™ MEM -α media in Hank’s buffer includes HBSS
Composition of HBSS buffer contains:- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
- Low level of glucose (1.00 g/ liter)
Application
Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.Formulation
additionally, to get access to detailed formulation visit ” formulation Tab” as well as by clicking here : MEM-alpha Formulation in Hank’s Buffer (HBSS)References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
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Minimum Essential Media (MEM) In Hanks’ Buffer
Minimum Essential Media (MEM) In Hanks’ Buffer
Background
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.PurMa™ MEM media in Hank’s buffer includes Hank’s buffer
Hanks buffer, it contains:- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM in Hanks’ Buffer contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
Applications
MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).Formulation
Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks’ BufferReferences
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
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MEM Alpha Modification in Earle’s Buffer (MEM-α)
MEM Alpha Modification in Earle’s Buffer (MEM-α)
Background
MEM Alpha Modification in Earle’s Buffer (MEM-α) certainly suits mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification applies for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.MEM -α contains
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
- Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
- Moreover, PurMaTM MEM -α media in Earle’s buffer includes Earles’ buffer
The composition of Earle’s buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
Additionally, MEM Alpha Modification in Earle’s Buffer (MEM-α)
it contains:- Low level of glucose (1.00 g/ liter)
- More importantly, Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)’
- Also, PurMa Biologics manufactures 118 types of MEM-α
Application
This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, Alpha modification of Minimum Essential Medium Eagle has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast, We stand behind our cell culture media. Our products are the outcome of 30 years of experience in cutting-edge science. PurMa Biologics manufactures over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we use %99 pure amino acids. This will significantly increase the consistency of your experiments. Considering the highest quality, our prices certainly are extremely reasonable.Formulation
Complete formulation is in the ” Formulation tab” as well as here: MEM-alpha Formulation in Earle’s BufferReferences
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay et al. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
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Leibovitz’s L-15 Medium
Leibovitz’s L-15 Medium
Background
Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.This media contains:
- High Level of Sodium pyruvate (5mM)
- Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.
- Basically, the buffering Capacity is maintained by other elements, mainly phosphates.
- And finally Using Galactose instead of glucose makes this media quite different.
Application of Leibovitz’s L-15
- Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
- Establishing human tumor cells lines such as Hep-2 cells.
- As a supplement in sterile cell media for the dissection and isolation of kidney cells.
- As an important media which provides proper buffering system for culturing of lung cells.
- A valid option for growing fish originated from cell lines.
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting-edge science. PurMa Biologics routinely manufactures over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We use %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.Formulation
For complete formulation please visit the “formulation tab”, as well as by clicking here: Leibovitz’s L-15 Formulation -
William's E Medium
Williams′ Medium E
Background
Williams′ Medium E is a modification version of Williams′ Medium. Although it is like MEM, there is differences in glucose as well as amino acid contents. This media was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been shown that it is suited for longer-term adult primary cell culture.PurMa™ William’s E Medium is
- Suited for long-term culture.
- Enriched in amino acids.
- Also, double the glucose compared to the original formulation.
- Moreover, contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione as well as lipid methyl linoleate
Moreover, PurMa™ William’s E Medium also contains:
- Low level of glucose (2.00 g/ liter). PurMa Biologics manufacturers the high glucose (4.5 g/L) version of this media as well (Cat #: P3S029880)
- Although the standard formulation contains 2.2 g/L Sodium bicarbonate, PurMa Biologics manufacturers the high sodium bicarbonate (3.7 g/L) Williams′ Medium E as well (Cat #: P3S032880)
- 2 mM L-glutamine (292 mg/L)’
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McCoy’s 5A Modified Media (M5AMM)
McCoy’s 5A Modified Media (M5AMM)
Background
McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. moreover, our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality MCCOY’S 5A Contains:- High glucose (3g/L)
- Non-Essential Amino Acids
- Phenol Red
- L-glutamine
Application
McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).Formulation
Additionally, the complete formulation is available in the formulation tab as well as here: McCoy’s 5A FormulationReferences
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
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Eagle's Minimal Essential Medium (EMEM)
Eagle’s Minimum Essential Medium (EMEM)
Background
Eagle’s Minimal Essential Medium (EMEM) was developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Several versions of EMEM have been developed by modification of its composition. This media is suitable for a wide range of mammalian cells - adhesive as well as suspension cells.Ingredients
Moreover, Eagle's Minimal Essential Medium (EMEM) Medium contains:- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (1.5 g/L)
- Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
- 2 mM L-glutamine (292 mg/L)
- 1mM sodium pyruvate (0.11g/L)
- Additionally, a higher concentration of amino acids, vitamins, inositol, and choline are present in this medium.
Why PurMa Biologics? Why we stand behind our cell culture media.
Our products are the outcome of 30 years of experience in cutting-edge cell and tissue culture science. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. Considering the highest quality, our prices are extremely reasonable.Application of EMEM
Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cell lines. The dedicated scientists at PurMa Biologics have decades of experience with this media for culturing primary in addition to commercial cell lines, including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.Formulation
Click here for the complete formulation: EMEM Formulation -
Iscove's Modified Dulbecco's Medium (IMDM)
Although Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating cell lines, it is certainly quite suitable for slow-growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore making it an excellent choice for adhesive cells. scove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, high-density cell lines. Therefore, it is excellent for Jurkat, Hek, COS-7, and macrophage cells. IMDM is a modified and more advanced version of DMEM. Additionally, it includes selenium as well as additional amino acids and vitamins. it is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore it makes it excellent for adhesive cells.Ingredients
Iscove's Modified Dulbecco's Medium (IMDM) contains:- High Buffering Capacity with High NaHCO3 as well as High HEPES
- Sodium Bicarbonate (3.02 g/L)
- 5.9 g/L HEPES
- High glucose 4.5g/L
- Non-Essential Amino Acids
- 110 mg/L Sodium pyruvate (1.0 mM)
- 15 mg/L Phenol Red
- 0.584 g/L L-glutamine
Formulation
Complete formulation of IMDM is available on our website as well as here: IMDM FormulationReferences
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
PurMa™ Cell Culture Media contains commonly used, well described media for growing various primary as well as commercial cell lines.
Our Cell Culture line includes:
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- DMEM/F-12 (Dulbecco’s Modified Eagle’s Medium with Nutrient Mixture F-12)
- HAM’S F-12K (Kaighn’s Modification of Ham’s F-2 Medium)
- RPMI 16400 (Roswell Park Memorial Institute 1640)
- IMDM (Iscove’s Modified Dulbecco’s Medium)
- EMEM (Eagle’s Minimal Essential Medium)
- McCoy’s 5A Modified Media
- Williams’ E Medium
- Leibovitz’s L-15 Medium
- GMEM (Glasgow’s Modified Eagle’s Medium – Glutamine Free)
- MEM (Minimum Essential Media)
- MEM-α (Minimum Essential Media Alpha)
- MEM-α-N (Minimum Essential Media Alpha with Nucleosides)
- BME (Basal Medium Eagle).
PurMa™ MEM, MEM-α, MEM-α-N, and BME cell culture medias are available in either EBSS (Earle’s Balanced Salt Solution) or HBSS (Hanks’ Balanced Salt Solution).