Advanced DMEM/F12

PurMaTM Advanced DMEM/F12 contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced DMEM/F12, L-Alanyl-L-Glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Advanced DMEM/F12 contains:

Advanced DMEM/F12 is a substantial upgrade to the standard and MAX for of DMEM/F12. That is because this media contains ingredients to allow for serum reduction, specifically:

  1. All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  2. Ethanolamine, glutathione.
  3. Trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
  1. PurMaTM Albumin:  Chromatographically purified, lipid rich with IgG content <=0.1.0%.
  2. PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  3. PurMaTM Insulin Recombinant
  4. Trace of other elements which mimic the elements in fetal bovine serum.

Advanced form of DMEM/F12 is a significant step toward a chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined DMEM/F12 (CD-DMEM/F12) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD-DMEM/F12 will soon be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is here: Advanced DMEM F-12


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  6. Protective layer formation on magnesium in cell culture medium. Wagener V, Virtanen S.Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
  7. The viability change of pigskin in vitro. Ge L, Sun L, Chen J, Mao X, Kong Y, Xiong F, Wu J, Wei H. 2010 Jun;36(4):533-8. doi: 10.1016/j.burns.2009.08.001.PMID: 19836142
  8. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Soleimani M, Nadri S.Nat Protoc. 2009;4(1):102-6. doi: 10.1038/nprot.2008.221.PMID: 19131962
  9. Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests? Rohanová D , Boccaccini AR , Horkavcová D , Bozděchová P , Bezdička P , Častorálová M .J Mater Chem B. 2014 Aug 21;2(31):5068-5076. doi: 10.1039/c4tb00187g. Epub 2014 Jul 1.PMID: 32261840
  10. Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells. Wu X, Lin M, Li Y, Zhao X, Yan F. 2009 Mar;59(2):103-11. doi: 10.1007/s10616-009-9200-5. Epub 2009 Jun 4.PMID: 19496017
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature