Description

PurMaTM Advanced MEM in (Hank’s) HBSS Buffer contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced MEM in HBSS Buffer, L‑alanine‑L‑glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced MEM in HBSS Buffer contains:

  • All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture including:

Advanced MEM in HBSS Buffer is a substantial upgrade to the standard and MAX for of MEM in HBSS Buffer. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid-rich PurMaTM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

  1. PurMaTM Albumin:  Chromatographically purified with IgG content <=0.1.0%.
  1. PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  2. PurMaTM Insulin Recombinant
  3. Trace of other elements which mimic the elements in fetal bovine serum.

In other words, the advanced form of MEM is a significant step toward a chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined MEM in HBSS Buffer (CD- MEM in HBSS Buffer) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD-MEM soon will be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation: Complete formulation is available here: Advanced MEM in HBSS Buffer formulation

References:

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, Byard R, Dardick I, Tsang BK. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
  3. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
  4. An experimental model for ovarian tumor invasion of cultured mesothelial cell monolayer. Sawada M, Shii J, Akedo H, Tanizawa O. Lab Invest. 1994 Mar;70(3):333-8. PMID: 8145527
  5. Biocompatibility of trypan blue with human corneal cells. van Dooren BT, Beekhuis WH, Pels E. Arch Ophthalmol. 2004 May;122(5):736-42. doi: 10.1001/archopht.122.5.736. PMID: 15136322
  6. Induction of osteogenic protein-1 expression by interleukin-1beta in cultured rabbit articular chondrocytes. Yoshida S, Kubota Y, Toba T, Horiuchi S, Shimamura T. Tohoku J Exp Med. 2002 Jun;197(2):101-9. doi: 10.1620/tjem.197.101. PMID: 12233782
  7. Heat-stabilized albumin microspheres as a sustained drug delivery system for the antimetabolite, 5-fluorouracil. Truter EJ. Artif Cells Blood Substit Immobil Biotechnol. 1995;23(5):579-86. doi: 10.3109/10731199509117972. PMID: 8528451
  8. Roles of transforming growth factor beta in inhibition of androgen-induced growth of Shionogi carcinoma cells in serum-free medium. Yamanishi H, Nonomura N, Tanaka A, Yasui T, Nishizawa Y, Matsumoto K, Sato B. Cancer Res. 1990 Oct 1;50(19):6179-83. PMID: 2169337
  9. Leucine suppresses acid-induced protein wasting in L6 rat muscle cells. Bevington A, Brown J, Walls J. Eur J Clin Invest. 2001 Jun;31(6):497-503. doi: 10.1046/j.1365-2362.2001.00845.x. PMID: 11422399
  10. In vitro steroidogenesis by granulosa cells from equine pre-ovulatory follicles. Tucker KE, Henderson KA, Duby RT. J Reprod Fertil Suppl. 1991;44:45-55. PMID: 1795289
  11. Highly polarized secretion of inhibin by Sertoli cells in vitro. Handelsman DJ, Spaliviero JA, Kidston E, Robertson DM. 1989 Aug;125(2):721-9. doi: 10.1210/endo-125-2-721. PMID: 2546746
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature