Advanced Williams’ E Medium contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-Williams’ E needs 2-5% FBS and provides more consistency.
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Advanced Williams’ E Medium
$40.10 – $947.87
Description
Advanced Williams’ E Medium
Background
PurMaTM Advanced Williams’ E Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Williams’ E, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Williams’ E Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-Williams’ E incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Williams’ E Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Williams’ E (CD-A-Williams’ E) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available: Advanced Williams’ E Medium Formulation
References
- Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, et al. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8. PMID:
- Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277
- Serum-free culture of rabbit meniscal fibrochondrocytes: proliferative response. Webber RJ, Zitaglio T, Hough AJ Jr. J Orthop Res. 1988;6(1):13-23. doi: 10.1002/jor.1100060103. PMID: 333473
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Formulation
Quality Control
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Background
McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the and the full control you need for mammalian cell culture. MCCOY’S 5A Contains:- High glucose (3g/L)
- Non-Essential Amino Acids
- Phenol Red
- L-glutamine
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.Application
McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).Formulation
complete formulation is available in formulation tab as well as here:McCoy's 5A FormulationReferences
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
- In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Kaighn's Modification of Ham's F-12-K Medium
Background
Kaighn's Modification of Ham's F-12-K Medium is a modified version of Ham’s F-12 medium. originally, Ham’s F-12K (Kaighn’s) was used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. but, however, recently this media is used for a much larger variety of cells. Moreover, Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate.Ingredients:
F-12K Medium also contains:
– Low level of glucose (1.26 g/ liter) – Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed). – Additionally, PurMa Biologics also offers F-12K Medium with different level of sodium bicarbonate : 1.5 g/L (Cat # P3S057108) as well as, 3.7g/L (Cat # P3S032108) – 2 mM L-glutamine (292 mg/L)’ – A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.Why PurMa Biologics? Why we stand behind our Cell Culture Media:
Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. Also, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. We routinely manufacture over 1500 media, which is the most comprehensive collection of cell and tissue culture media. Considering the highest quality, our prices are extremely reasonable.Application of Ham’s F-12K:
This media has elements that prevent clumping, therefore, it is ideal for culturing primary as well as commercial cell lines, for instance normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells.Formulation:
For complete formulation click here: Ham's F-12K FormulationReferences:
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
- Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
- Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Advanced Leibovitz’s L-15 Medium
Background
PurMaTM Advanced Leibovitz’s L-15 Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Leibovitz’s L-15, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.Contents
Advanced Leibovitz’s L-15 Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Formulation
Complete formulation is available here: Advanced Leibovitz's L-15 FormulationReferences
- The effect of a phorbol ester on amphibian myogenesis. Zeng MB, et al. 1990 Mar;23(1):106-15. PMID: 2382522.
- Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish. Ishida AT, Cheng MH.Exp Eye Res. 1991 Feb;52(2):175-91. doi: 10.1016/0014-4835(91)90257-f.PMID: 2013300
[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read moreParameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature
Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)
Background
PurMaTM Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-IMDM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.Contents
Advanced IMDM Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-IMDM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Formulation
Complete formulation of Advanced IMDM: Advanced IMDM FormulationReferences
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, et al. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read moreParameter Specification Appearance Red, clear liquid pH 7.2 ± 0.1 Osmolality 275-360 mOsm/L Endotoxin NMT< 2EU/mL Mycoplasma Negative Suitability Suitable for mammalian cell culture Additive Sodium pyruvate Indicator Phenol red Mycoplasma Detection Negative Sterility Tested Sterile filtered using 0.22 µm filter Form Liquid Shipping Condition Room temperature Title
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