Basal Medium Eagle (BME) in Earle’s Buffer

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Basal Medium Eagle (BME) in Earle’s Buffer

Name: Advanced Eagle's Minimal Essential Medium (A-EMEM)
Availability: InStock

Background

Basal Medium Eagle (BME) in Earle’s Buffer originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle’s as well as in Hanks' salts for use without CO₂.

Ingredients

PurMa™ BME media in Earle's buffer contains:

Earl's buffer includes:

Calcium chloride
Magnesium sulfate
Potassium chloride
Sodium bicarbonate
Sodium chloride
and lastly, Sodium Phosphate, monobasic

PurMa Biologics so far, manufactures 109 types BME in Earle’s buffer.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. undoubtly, considering the highest quality, our prices certainly are extremely reasonable.

Application

BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.

Formulation

for complete formulation click here, alternatively you can find it in “Formulation Tab”: BME with Earle's Buffer Formulation References:

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

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Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

Background

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of EMEM-MAX over EMEM are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: EMEM-MAX Formulation

References

Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, et al. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, et al. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, et al. Histochem Cytobiol. 1988;26(3):123-31. PMID:

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Minimum Essential Media (MEM) In Hanks’ Buffer

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Minimum Essential Media (MEM) In Hanks’ Buffer

Background

Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

PurMa^™ MEM media in Hank’s buffer includes Hank's buffer

Hanks buffer, it contains:

Potassium Chloride
Potassium Phosphate, monobasic
Sodium Bicarbonate
Sodium Chloride
Sodium Phosphate, dibasic

Additionally, MEM in Hanks’ Buffer contains:

Low level of glucose (1.00 g/ liter)
Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
2 mM L-glutamine (292 mg/L)

Moreover, PurMa Biologics manufactures 122 types of MEM.

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Applications

MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).

Formulation

Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks' Buffer

References

Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

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Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

Background

PurMa^TM Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced BME. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Consequently, Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research. Furthermore, it will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

The complete formulation can be found here: Advanced BME in Hanks' Buffer (HBSS) Formulation

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Condition
Format
Size
	Clear selection

Advanced Eagle's Minimal Essential Medium (A-EMEM)

Advanced Eagle’s Minimal Essential Medium (A-EMEM)

Description

Advanced Eagle’s Minimal Essential Medium (A-EMEM)

Background

Contents

Advanced EMEM Contains:

Crucial Ingredients:

Formulation

References

Formulation

Quality Control

Reviews

SDS

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