PurMaTM Advanced EMEM contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced EMEM, L‑alanine‑L‑glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced EMEM contains:

  • All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture including:

Advanced EMEM is a substantial upgrade to the standard and MAX for of EMEM. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich PurMaTM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

  1. PurMaTM Albumin:  Chromatographically purified with IgG content <=0.1.0%.
  1. PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  2. PurMaTM Insulin Recombinant
  3. Trace of other elements which mimic the elements in fetal bovine serum.

In another word, advanced form of EMEM is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined EMEM (CD- EMEM) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD- EMEM soon will be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is available here: Advanced EMEM Formulation


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  3. Insulin-like growth factor binding protein-1 from Hep G2 cells is potently inhibited by the truncated IGF-I analogue des-(1-3) IGF-I. Lindgren BF, Isaksson M, Stern I, Hall K.Acta Endocrinol (Copenh). 1993 Jan;128(1):81-7. doi: 10.1530/acta.0.1280081.PMID:
  4. Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium. Pietrzkowski Z, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):143-52. PMID: 3197876.
  5. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, Reiss K, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID:
  6. Improved method for the isolation and cultivation of human scalp dermal papilla cells. Warren R, Chestnut MH, Wong TK, Otte TE, Lammers KM, Meili ML. J Invest Dermatol. 1992 May;98(5):693-9. doi: 10.1111/1523-1747.ep12499909. PMID:
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Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature