Advanced Eagle’s Minimal Essential Medium (A-EMEM)

Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, A-EMEM needs 2-5% FBS and provides more consistency.

Description

Advanced Eagle’s Minimal Essential Medium (A-EMEM)

Background

PurMaTM Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-EMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced EMEM Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-EMEM incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Eagle’s Minimal Essential Medium (A-EMEM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced EMEM (CD-A-EMEM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced EMEM Formultaion

References

  1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
  2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-EMEM Standard Formulation, A-EMEM w/o L-Alanyl-L-Glutamine, A-EMEM w/o Phenol Red, A-EMEM w/o Sodium Bicarbonate, A-EMEM w/o Sodium Pyruvate, A-EMEM w/o Glucose, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-EMEM w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-EMEM High Glucose (4.5 g/L), A-EMEM High Sodium Bicarbonate (2.438 g/L), A-EMEM High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM 15 mM HEPES (3.6 g/L), A-EMEM 25 mM HEPES (5.9 g/L), A-EMEM 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, w/o Phenol Red, A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-EMEM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-EMEM w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-EMEM w/o Phenol Red, High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-EMEM w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM w/o Lipoic Acid

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced EMEM Formulation

SDS

SDS

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