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Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)

$39.86 – $754.64

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SKU: N/A Category: Cell & Tissue Culture Reagents

Description

Advanced IMDM

PurMa^TM Advanced IMDM contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes. In Advanced IMDM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced IMDM contains:

All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
It includes the elements which significantly reduce the need for a serum for cell culture including:

Advanced IMDM is a substantial upgrade to the standard and MAX for of IMDM. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich PurMa^TMAlbumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

PurMa^TMAlbumin: Chromatographically purified with IgG content <=0.1.0%.

PurMa^TMHuman Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
PurMa^TM Insulin Recombinant
Trace of other elements which mimic the elements in fetal bovine serum.

In another word, advanced form of IMDM is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined IMDM (CD-IMDM) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD-IMDM soon will be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation of Advanced IMDM: Advanced IMDM

References:

Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
Improved Survival and Initiation of Differentiation of Human Induced Pluripotent Stem Cells to Hepatocyte-Like Cells upon Culture in William’s E Medium followed by Hepatocyte Differentiation Inducer Treatment, Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige, PLoS One. 2016; 11(4): e0153435. Published online 2016 Apr 13. doi: 10.1371/journal.pone.0153435, PMCID: PMC4830564
Cell culture medium formulation and its implications in cancer metabolism,Tobias Ackermann, Saverio Tardito, Trends Cancer. Author manuscript; available in PMC 2019 Jun 10. Published in final edited form as: Trends Cancer. 2019 Jun 4; 5(6): 329–332. Published online 2019 May 29. doi: 10.1016/j.trecan.2019.05.004, PMCID: PMC6557711
Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080/0032472031000141250. PMID: 19746589
Essential components for ex vivo proliferation of mesenchymal stromal cells. Fekete N, Rojewski MT, Lotfi R, Schrezenmeier H. Tissue Eng Part C Methods. 2014 Feb;20(2):129-39. doi: 10.1089/ten.TEC.2013.0061. Epub 2013 Jul 5. PMID: 23713576
Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, Romein B, Luyben KC, van Dijken JP. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
Development of a serum-free medium for the production of humanized antibody from Chinese hamster ovary cells using a statistical design. Kim EJ, Kim NS, Lee GM. In Vitro Cell Dev Biol Anim. 1998 Nov-Dec;34(10):757-61. doi: 10.1007/s11626-998-0029-6.

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

Formulation

Advanced IMDM

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PurMa Primary Cell Culture Essential Media (PPEM)
This media is specifically designed to preserve and maintain the growth of primary cell including primary tumor cells. PurMa^TM
... This media is specifically designed to preserve and maintain the growth of primary cell including primary tumor cells. PurMa^TM

Primary cells, when they are harvested from the surrounding matrix have special requirements to overcome the growth of naturally surrounding filaments and the pathogens that come along with the neural cells at the time of extraction.

The first few hours (up to 24 hrs.) of harvesting primary neural cells is critical and require a combination of neurotrophic factors/ chemical elements to acclimate the new environment without losing their integrity and functional properties.

This media contains an additional neurotrophic factors/ chemical element whose impact is critical but temporary and does not interfere with the read-out of any drug being tested on neural cells.

Amino acids with 99% purity are individually added to the medium as a source of essential amino acids. PPEM includes additional amino acids as well as selenium which guarantees the adequate availability of these elements during the course of incubation.

PPEM requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS)

PPEM uses a sodium bicarbonate buffer system (3.025g/l), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Available as chemically defined (serum free)/ per request.

Available as powder.

PPEM contains L-glutamine and Phenol red.

For additional information, please contact PurMabiologics friendly technical team.

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Leibovitz’s L-15 Medium – MAX

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pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] Leibovitz’s L-15-MAX: In general, the advantages of Leibovitz’s L-15 -MAX over Regular Leibovitz’s L-15 – is replacing L-Alanyl-L-Glutamine with L-Glutamine, therefore:

Possess longer shelf life)
Minimizes toxic ammonia build-up.
Improves cell viability and growth.
Remains stable across a wide range of temperatures.
Minimizes the growth of pathogens by stabilizing the PH.
Improves the culture of primary cells.

Additionally, Leibovitz’s L-15 -MAX contains:

5 mM sodium pyruvate
Non-Essential Amino Acids
PurMa Biologics Manufactures several types of Leibovitz’s L-15-MAX based on the
Phenol Red
Galactose

L-glutamine is a vital amino acid that is supplemented with mammalian cell culture media. This important amino acid has several important functions including the production of purine and pyrimidine nucleotides amino sugars, and glutathione as well as performing as the secondary source of energy. The latter is more crucial in cells dividing rapidly. One drawback of using L-glutamine is that in solutions such as cell culture media, L-glutamine degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. The speed of degradation of L-glutamine depends on time, temperature, and pH. One way to minimize the degradation of L-glutamine degradation in media is to gradually add these amino acids to your cell culture but has to be calculated to keep the level of L-glutamine at a constant level. Practically, this strategy even though is possible but tedious, and depending on the volume of cell culture work in your la, keeping track of time to keep the level of L-glutamine at the same level is difficult and could be tedious if not impossible. A viable and well-proven alternative for using L-glutamine, which is less sensitive to environmental factors such as pH, is applying L-alanyl-L-glutamine, which is more stable in aqueous solutions and does not easily degrade and cells gradually release aminopeptidases that hydrolyze the dipeptide, slowly releasing L-alanine and L-glutamine into the culture media. The resulting L-glutamine and L-alanine are then used by the cells for protein production or in the TCA cycle. PurMa^TMGluaSup (Cat# P3S210 559) is a proprietary combination of L-alanyl-L-glutamine and other elements which prevent degradation of L-glutamine in the period when is released from L-alanyl-L-glutamine before being utilized by cells. Using PurMa^TMGluaSup maximizes energy metabolism and high growth yield. The other components in PurMa^TMGluaSup neutralize the trace of ammonia on your cells whose generation in cell culture is inevitable in presence of L-glutamine and harmful to the cells. Additionally, PurMa^TMGluaSup offers more buffering capacity which maintains physiological pH more efficiently. An extra buffering system in this media is a powerful tool to avoid changing the PH which results in instability in growing and cellular mechanisms. Changing the PH is also one of the most common factors for growing mold and other pathogens. Leibovitz’s L-15 -MAX functions better than regular Leibovitz’s L-15 on primary cell culture and most of the commercial cell lines such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Leibovitz’s L-15 -MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS. In general, The complete formulation can be found here: Leibovitz's L-15 MAX References

Cryoprotective effects of antifreeze proteins delivered into zebrafish embryos. Martínez-Páramo S, Barbosa V, Pérez-Cerezales S, Robles V, Herráez MP. 2009 Apr;58(2):128-33. doi: 10.1016/j.cryobiol.2008.11.013. Epub 2008 Dec 24.PMID: 19135991
A practical guide for assessing respiratory burst and phagocytic cell activity in the fathead minnow, an emerging model for immunotoxicity. Hampton LMT, Jeffries MKS, Venables BJ. 2020 Jul 10;7: 100992. doi: 10.1016/j.mex.2020.100992. eCollection 2020. PMID: 3271485
The effects of insulin, transferrin and androgens on rat prostate explants in serum-free organ culture. Nguyen-Le XK, Brière N, Corcos J. 1997;6(3):339-49. doi: 10.1002/biof.5520060304. PMID: 9288404.
Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière N, Droz D.Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265
Elasticity and structure of eukaryote chromosomes studied by micromanipulation and micropipette aspiration. Houchmandzadeh B, et al. J Cell Biol. 1997. PMID: 9314524
The effect of a phorbol ester on amphibian myogenesis. Zeng MB, Tseng MP. Shi Yan Sheng Wu Xue Bao. 1990 Mar;23(1):106-15. PMID: 2382522.
Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish. Ishida AT, Cheng MH.Exp Eye Res. 1991 Feb;52(2):175-91. doi: 10.1016/0014-4835(91)90257-f.PMID: 2013300
The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata. Sivakumar S, Raja Swaminathan T, Kumar R, Kalaimani N.J Aquat Anim Health. 2019 Sep;31(3):244-258. doi: 10.1002/aah.10073. Epub 2019 Aug 22.PMID: 31441117
Expansion of human hematopoietic cells from umbilical cord blood using roller bottles in CO2 and CO2-free atmosphere. Andrade-Zaldívar H, Kalixto-Sánchez MA, de la Rosa AP, De León-Rodríguez A.Stem Cells Dev. 2011 Apr;20(4):593-8. doi: 10.1089/scd.2010.0236. Epub 2010 Nov 8.PMID: 20825280
Growth of fish cell lines in glutamine-free media. Bols NC, Ganassin RC, Tom DJ, Lee LE. 1994;16(3):159-66. doi: 10.1007/BF00749903.PMID: 7766144

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL Read more

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] In general, the advantages of EMEM MAX over Regular EMEM are as follows:

PH Capacity. The standard form of EMEM-MAX contains 2.5 g/L of Sodium Bicarbonate. PurMa Biologics also manufactures EMEM with higher NaHCO3 (2.438 g/L) which provides higher protection against PH changes (Cat: P3M132110)

Contains 2 mM L-Alanyl-L-Glutamine (0.434 g/L) instead of L-Glutamine and therefore:

Possess longer shelf life)
Minimizes toxic ammonia build-up.
Improves cell viability and growth.
Remains stable across a wide range of temperatures.
Minimizes the growth of pathogens by stabilizing the PH.
Improves the culture of primary cells.

Additionally, EMEM - MAX contains:

2 mM L-Alanyl-L-Glutamine (434 mg/L) (therefore possess longer shelf life)
Non-Essential Amino Acids
Sodium Bicarbonate (1.50 g/L)
1 mM sodium pyruvate
Non-Essential Amino Acids
PurMa Biologics Manufactures several types of EMEM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity) requested.
Phenol Red
Moreover, High Glucose DMEM-MAX (4.5g /L) is available at PurMa Biologics (Cat: P3M129110)

L-glutamine is a vital amino acid that is supplemented with mammalian cell culture media. This important amino acid has several important functions including the production of purine and pyrimidine nucleotides amino sugars, and glutathione as well as performing as the secondary source of energy. The latter is more crucial in cells dividing rapidly. One drawback of using L-glutamine is that in solutions such as cell culture media, L-glutamine degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. The speed of degradation of L-glutamine depends on time, temperature, and pH. One way to minimize the degradation of L-glutamine degradation in media is to gradually add these amino acids to your cell culture but has to be calculated to keep the level of L-glutamine at a constant level. Practically, this strategy even though is possible but tedious, and depending on the volume of cell culture work in your la, keeping track of time to keep the level of L-glutamine at the same level is difficult and could be tedious if not impossible. A viable and well-proven alternative for using L-glutamine, which is less sensitive to environmental factors such as pH, is applying L-alanyl-L-glutamine, which is more stable in aqueous solutions and does not easily degrade and cells gradually release aminopeptidases that hydrolyze the dipeptide, slowly releasing L-alanine and L-glutamine into the culture media. The resulting L-glutamine and L-alanine are then used by the cells for protein production or in the TCA cycle. PurMa^TMGluaSup (Cat# P3S210 559) is a proprietary combination of L-alanyl-L-glutamine and other elements which prevent degradation of L-glutamine in the period when is released from L-alanyl-L-glutamine before being utilized by cells. Using PurMa^TMGluaSup maximizes energy metabolism and high growth yield. The other components in PurMa^TMGluaSup neutralize the trace of ammonia on your cells whose generation in cell culture is inevitable in presence of L-glutamine and harmful to the cells. Additionally, PurMa^TMGluaSup offers more buffering capacity which maintains physiological pH more efficiently. An extra buffering system in this media is a powerful tool to avoid changing the PH which results in instability in growing and cellular mechanisms. Changing the PH is also one of the most common factors for growing mold and other pathogens. EMEM-MAX functions better than regular EMEM on primary cell culture and most of the commercial cell lines such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. EMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS. In general, EMEM -MAX contains:

2 mM L-Alanyl-L-Glutamine (434 mg/L) (therefore possess longer shelf life)
1mM mM sodium pyruvate (1100 mg/L)
Low glucose (1000 mg/L). For higher glucose please order P3M129110 EMEM
Non-Essential Amino Acids
Sodium Bicarbonate (1.5g/L), for higher NAHCO3 (2.438 g/L), please order EMEM, Cat: P3M159110
Phenol Red
Lipoic Acid

The complete formulation can be found here: EMEM MAX Formulation References:

Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
Insulin-like growth factor binding protein-1 from Hep G2 cells is potently inhibited by the truncated IGF-I analogue des-(1-3) IGF-I. Lindgren BF, Isaksson M, Stern I, Hall K.Acta Endocrinol (Copenh). 1993 Jan;128(1):81-7. doi: 10.1530/acta.0.1280081.PMID:
Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium. Pietrzkowski Z, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):143-52. PMID: 3197876.
Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, Reiss K, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID:
Improved method for the isolation and cultivation of human scalp dermal papilla cells. Warren R, Chestnut MH, Wong TK, Otte TE, Lammers KM, Meili ML. J Invest Dermatol. 1992 May;98(5):693-9. doi: 10.1111/1523-1747.ep12499909. PMID:
Human lens epithelial cell proliferation in a protein-free medium. Wormstone IM, Liu CS, Rakic JM, Marcantonio JM, Vrensen GF, Duncan G.Invest Ophthalmol Vis Sci. 1997 Feb;38(2):396-404.PMID:

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

McCoy’s 5A Modified Media (M5AMM)

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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] PurMa^TM McCoy’s 5A Medium was originally used to perform analysis on Walker Carcinosarcoma 256 Cells, and subsequently modified to create a new medium known as McCoy’s 5A Medium. PurMa^TMMcCoy’s 5A Medium contains several folds of amino acids and vitamins compared to the original formulation. There are several versions of McCoy’s 5A available in market. McCoy’s 5A produced by PurMa Biologics covers the main needs for most of the cell’s lines, delivers consistency, excellent quality, and the and the full control that needed for mammalian cell culture. PurMa^TMMcCoy’s 5A contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). This Advanced MCCOY’S 5A contains:

High glucose (3g/L)
Non-Essential Amino Acids
Phenol Red
L-glutamine

Why PurMa Biologics? Why we stand behind our cell culture media:

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
Considering the highest quality, our prices are extremely reasonable.

Application of PurMa^TM McCoy’s This media has elements that prevent clumping the cells which is a necessity for performing analysis on these cells. Ham’s F-12K has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated PurMa^TM McCoy’s medium for the culture of vast varieties of primary Skin cells, insect cells, as well as bovine preantral follicles, post implantation hamster embryos. McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa and Jurkat cells. PurMa^TM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Formulation: for complete formulation click here: Regular McCoy Formulation

The survival of human skin stored by refrigeration at 4 degrees C in McCoy's 5A medium: does oxygenation of the medium improve storage time? DeBono R, Rao GS, Berry RB.Plast Reconstr Surg. 1998 Jul;102(1):78-83. doi: 10.1097/00006534-199807000-00012.PMID: 9655410
Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
Fresh and vitrified bovine preantral follicles have different nutritional requirements during in vitro culture. Castro SV, Carvalho AA, Silva CM, Santos FW, Campello CC, Figueiredo JR, Rodrigues AP.Cell Tissue Bank. 2014 Dec;15(4):591-601. doi: 10.1007/s10561-014-9432-2. Epub 2014 Mar 8.PMID: 24610241
In vitro culture of postimplantation hamster embryos. Ebron-McCoy MT, Beyer PE, Oglesby LA, Kavlock RJ.Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
The turkey myogenic satellite cell: optimization of in vitro proliferation and differentiation. McFarland DC, Doumit ME, Minshall RD.Tissue Cell. 1988;20(6):899-908. doi: 10.1016/0040-8166(88)90031-6.PMID: 3245037
Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, Glass JR, Fuller DJ, Gerner EW. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115. PMID: 3958058

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

Advanced Iscove's Modified Dulbecco's Medium (A-IMDM)