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  Trypsin-EDTA (Phenol Red)

  [fusion_builder_container type="flex" hundred_percent="no" equal_height_columns="no" menu_anchor="" hide_on_mobile="small-visibility,medium-visibil... [fusion_builder_container type="flex" hundred_percent="no" equal_height_columns="no" menu_anchor="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" background_color="" background_image="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" parallax_speed="0.3" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" overlay_color="" video_preview_image="" border_color="" border_style="solid" padding_top="" padding_bottom="" padding_left="" padding_right=""][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" background_position="left top" background_color="" border_color="" border_style="solid" border_position="all" spacing="yes" background_image="" background_repeat="no-repeat" padding_top="" padding_right="" padding_bottom="" padding_left="" margin_top="0px" margin_bottom="0px" class="" id="" animation_type="" animation_speed="0.3" animation_direction="left" hide_on_mobile="small-visibility,medium-visibility,large-visibility" center_content="no" last="true" min_height="" hover_type="none" link="" border_sizes_top="" border_sizes_bottom="" border_sizes_left="" border_sizes_right="" first="true"][fusion_text] Trypsin is one of the members of serine protease family which is mainly made in intestine and activated in pancreases and widely used to dissociate the adherent cells for routine cell culture. Members of Trypsin family are structurally highly similar. Trypsin cleaves their substrates after lysine and arginine residues (IGDKLIPA) unless these amino acids are followed by a proline. Optimum activity is achieved at 37oC and at the PH 7.3-8.5. PurMa^TM Trypsin is extracted from bovine pancreas using the most sophisticated technologies with over 98 percent purity. HPLC purified PurMa^TM Trypsin does not contain the enzymes and elements that cause mutations in cells gradually during several passages. Moreover, Optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40° and 45° which cause the deactivation of trypsin in a temperature dependent manner. Therefore, all the buffers needed in this process should be calcium and magnesium free to proper function of this enzyme. The activity of this enzyme is further modulated by the sulfation of Tyr-154. Features and Benefits - PurMa^TM Trypsin-EDTA (0.25%), Phenol Red does not contain calcium or magnesium ions. - This product does not contain serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF. - This product does not contain Phytates or salts of phytic acid, such as myo-inositol which is commonly present in cell culture media and inhibits trypsin activity. - Has been rigorously been evaluated for mycoplasma /endotoxins contaminations. PurMa^TM Trypsin-EDTA (0.25%), Phenol Red is effective and easy to use [/fusion_text][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 100 ml, 6 x 100 ml, 1 x 500 mL, 6 x 500 mL Read more
• 

  Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

  [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] In general, the advantages of EMEM MAX over Regular EMEM are as follows:

  1. PH Capacity. The standard form of EMEM-MAX contains 2.5 g/L of Sodium Bicarbonate. PurMa Biologics also manufactures EMEM with higher NaHCO3 (2.438 g/L) which provides higher protection against PH changes (Cat: P3M132110)

  2. Contains 2 mM L-Alanyl-L-Glutamine (0.434 g/L) instead of L-Glutamine and therefore:

  • Possess longer shelf life)
  • Minimizes toxic ammonia build-up.
  • Improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Minimizes the growth of pathogens by stabilizing the PH.
  • Improves the culture of primary cells.

  Additionally, EMEM - MAX contains:

  • 2 mM L-Alanyl-L-Glutamine (434 mg/L) (therefore possess longer shelf life)
  • Non-Essential Amino Acids
  • Sodium Bicarbonate (1.50 g/L)
  • 1 mM sodium pyruvate
  • Non-Essential Amino Acids
  • PurMa Biologics Manufactures several types of EMEM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity) requested.
  • Phenol Red
  • Moreover, High Glucose DMEM-MAX (4.5g /L) is available at PurMa Biologics (Cat: P3M129110)

  L-glutamine is a vital amino acid that is supplemented with mammalian cell culture media. This important amino acid has several important functions including the production of purine and pyrimidine nucleotides amino sugars, and glutathione as well as performing as the secondary source of energy. The latter is more crucial in cells dividing rapidly. One drawback of using L-glutamine is that in solutions such as cell culture media, L-glutamine degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. The speed of degradation of L-glutamine depends on time, temperature, and pH. One way to minimize the degradation of L-glutamine degradation in media is to gradually add these amino acids to your cell culture but has to be calculated to keep the level of L-glutamine at a constant level. Practically, this strategy even though is possible but tedious, and depending on the volume of cell culture work in your la, keeping track of time to keep the level of L-glutamine at the same level is difficult and could be tedious if not impossible. A viable and well-proven alternative for using L-glutamine, which is less sensitive to environmental factors such as pH, is applying L-alanyl-L-glutamine, which is more stable in aqueous solutions and does not easily degrade and cells gradually release aminopeptidases that hydrolyze the dipeptide, slowly releasing L-alanine and L-glutamine into the culture media. The resulting L-glutamine and L-alanine are then used by the cells for protein production or in the TCA cycle. PurMa^TM GluaSup (Cat# P3S210 559) is a proprietary combination of L-alanyl-L-glutamine and other elements which prevent degradation of L-glutamine in the period when is released from L-alanyl-L-glutamine before being utilized by cells. Using PurMa^TM GluaSup maximizes energy metabolism and high growth yield. The other components in PurMa^TM GluaSup neutralize the trace of ammonia on your cells whose generation in cell culture is inevitable in presence of L-glutamine and harmful to the cells. Additionally, PurMa^TM GluaSup offers more buffering capacity which maintains physiological pH more efficiently.  An extra buffering system in this media is a powerful tool to avoid changing the PH which results in instability in growing and cellular mechanisms. Changing the PH is also one of the most common factors for growing mold and other pathogens. EMEM-MAX functions better than regular EMEM on primary cell culture and most of the commercial cell lines such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. EMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS. In general, EMEM -MAX contains:

  • 2 mM L-Alanyl-L-Glutamine (434 mg/L) (therefore possess longer shelf life)
  • 1mM mM sodium pyruvate (1100 mg/L)
  • Low glucose (1000 mg/L). For higher glucose please order P3M129110 EMEM
  • Non-Essential Amino Acids
  • Sodium Bicarbonate (1.5g/L), for higher NAHCO3 (2.438 g/L), please order EMEM, Cat: P3M159110
  • Phenol Red
  • Lipoic Acid

  The complete formulation can be found here: EMEM MAX Formulation References:

  1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
  2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
  3. Insulin-like growth factor binding protein-1 from Hep G2 cells is potently inhibited by the truncated IGF-I analogue des-(1-3) IGF-I. Lindgren BF, Isaksson M, Stern I, Hall K.Acta Endocrinol (Copenh). 1993 Jan;128(1):81-7. doi: 10.1530/acta.0.1280081.PMID:
  4. Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium. Pietrzkowski Z, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):143-52. PMID: 3197876.
  5. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, Reiss K, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID:
  6. Improved method for the isolation and cultivation of human scalp dermal papilla cells. Warren R, Chestnut MH, Wong TK, Otte TE, Lammers KM, Meili ML. J Invest Dermatol. 1992 May;98(5):693-9. doi: 10.1111/1523-1747.ep12499909. PMID:
  7. Human lens epithelial cell proliferation in a protein-free medium. Wormstone IM, Liu CS, Rakic JM, Marcantonio JM, Vrensen GF, Duncan G.Invest Ophthalmol Vis Sci. 1997 Feb;38(2):396-404.PMID:

  [/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

  Parameter	Specification
  Appearance	Red, clear liquid
  pH	7.2 ± 0.1
  Osmolality	275-360 mOsm/L
  Endotoxin	NMT< 2EU/mL
  Mycoplasma	Negative
  Suitability	Suitable for mammalian cell culture
  Additive	Sodium pyruvate
  Indicator	Phenol red
  Mycoplasma Detection	Negative
  Sterility Tested	Sterile filtered using 0.22 µm filter
  Form	Liquid
  Shipping Condition	Room temperature

  [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL Read more
• 

  Kaighn’s Modification of Ham’s F-12 Medium (Ham’s F-12K)

  [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundred_percent_height_scroll="no" align_content="stretch" flex_align_items="flex-start" flex_justify_content="flex-start" flex_column_spacing="" hundred_percent_height_center_content="yes" equal_height_columns="no" container_tag="div" menu_anchor="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" status="published" publish_date="" class="" id="" spacing_medium="" margin_top_medium="" margin_bottom_medium="" spacing_small="" margin_top_small="" margin_bottom_small="" margin_top="" margin_bottom="" padding_dimensions_medium="" padding_top_medium="" padding_right_medium="" padding_bottom_medium="" padding_left_medium="" padding_dimensions_small="" padding_top_small="" padding_right_small="" padding_bottom_small="" padding_left_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" link_color="" link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] Kaighn’s Modification of Ham’s F-12 Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) was originally used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine (therefore the media smells after incubated with cells after 48 hours), thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These compensated for supplemental serum. But the addition of 10 % FBS is necessary. PurMa^TM F-12K Medium contains no proteins or growth factors and therefore should be supplemented with FBS.  PurMa ^TM F-12K Medium also contains: -  Low level of glucose (1.26 g/ liter) -  Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed). - PurMa Biologics also offers F-12K Medium with low sodium bicarbonate (1.5 g/L) (Cat # P3S057108) as well as high sodium bicarbonate (3.7g/L) (Cat # P3S032108) -  2 mM L-glutamine (292 mg/L)’ - A higher concentration of amino acids, vitamins, inositol and choline are present in this medium. PurMa Biologics manufactures 96 types of Ham’s F-12K which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for Ham’s F-12K   media. Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

  Application of Ham’s F-12K   This media has elements that prevent clumping the cells which is a necessity for performing analysis on these cells. Ham’s F-12K has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated Ham’s F-12K  medium for the culture of vast varieties of primary as well as commercial cell lines including: normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. Ham’s F-12K   Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). Formulation: for complete formulation click here: Ham's F-12K Formulation

  • Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss TL, Sirbasku DA. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
  • Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss TL, Karey KP, Burleigh BD, Parker D, Sirbasku DA. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811. PMID:

  • Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong HK, Chick WL, Sato GH. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
  • Rat pituitary tumor cells in serum-free culture. I. Selection of thyroid hormone-responsive and autonomous cells. Riss TL, Stewart BH, Sirbasku DA. In Vitro Cell Dev Biol. 1989 Feb;25(2):127-35. doi: 10.1007/BF02626168. PMID:
  • Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277
  • Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production. Qi YM, Greenfield PF, Reid S. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660. PMID: 22358660.
  • Medium formulations. Curr Protoc Cell Biol. 2001 May; Appendix 2: Appendix 2B. doi: 10.1002/0471143030.cba02bs06. PMID: 18228282.
  • Serum-free culture of rabbit meniscal fibrochondrocytes: proliferative response. Webber RJ, Zitaglio T, Hough AJ Jr. J Orthop Res. 1988;6(1):13-23. doi: 10.1002/jor.1100060103. PMID: 3334734,

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  Parameter	Specification
  Appearance	Red, clear liquid
  pH	7.2 ± 0.1
  Osmolality	275-360 mOsm/L
  Endotoxin	NMT< 2EU/mL
  Mycoplasma	Negative
  Suitability	Suitable for mammalian cell culture
  Additive	Sodium pyruvate
  Indicator	Phenol red
  Mycoplasma Detection	Negative
  Sterility Tested	Sterile filtered using 0.22 µm filter
  Form	Liquid
  Shipping Condition	Room temperature

  [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL Read more
• 

  Advanced Eagle’s Minimal Essential Medium (A-EMEM)

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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""] PurMa^TM Advanced EMEM contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced EMEM, L‑alanine‑L‑glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced EMEM contains:

  • All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture including:

  Advanced EMEM is a substantial upgrade to the standard and MAX for of EMEM. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich PurMa^TM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

  1. PurMa^TM Albumin:  Chromatographically purified with IgG content <=0.1.0%.

  1. PurMa^TM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  2. PurMa^TM Insulin Recombinant
  3. Trace of other elements which mimic the elements in fetal bovine serum.

  In another word, advanced form of EMEM is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated. Chemically Defined EMEM (CD- EMEM) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD- EMEM soon will be offered globally to laboratories who are looking for consistency and repeatability among their data. Formulation: Complete formulation is available here: Advanced EMEM Formulation References:

  1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
  2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
  3. Insulin-like growth factor binding protein-1 from Hep G2 cells is potently inhibited by the truncated IGF-I analogue des-(1-3) IGF-I. Lindgren BF, Isaksson M, Stern I, Hall K.Acta Endocrinol (Copenh). 1993 Jan;128(1):81-7. doi: 10.1530/acta.0.1280081.PMID:
  4. Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium. Pietrzkowski Z, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):143-52. PMID: 3197876.
  5. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, Reiss K, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID:
  6. Improved method for the isolation and cultivation of human scalp dermal papilla cells. Warren R, Chestnut MH, Wong TK, Otte TE, Lammers KM, Meili ML. J Invest Dermatol. 1992 May;98(5):693-9. doi: 10.1111/1523-1747.ep12499909. PMID:
  7. Human lens epithelial cell proliferation in a protein-free medium. Wormstone IM, Liu CS, Rakic JM, Marcantonio JM, Vrensen GF, Duncan G.Invest Ophthalmol Vis Sci. 1997 Feb;38(2):396-404.PMID:

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  Parameter	Specification
  Appearance	Red, clear liquid
  pH	7.2 ± 0.1
  Osmolality	275-360 mOsm/L
  Endotoxin	NMT< 2EU/mL
  Mycoplasma	Negative
  Suitability	Suitable for mammalian cell culture
  Additive	Sodium pyruvate
  Indicator	Phenol red
  Mycoplasma Detection	Negative
  Sterility Tested	Sterile filtered using 0.22 µm filter
  Form	Liquid
  Shipping Condition	Room temperature

  [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL Read more