Basal Medium Eagle (BME) in Hanks’ Buffer

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Basal Medium Eagle (BME) in Hanks’ BufferBasal Medium Eagle (BME) in Hanks’ Buffer

Basal Medium Eagle (BME) in Hanks’ Buffer

$35.99 – $683.88

Basal Medium Eagle (BME) in Hanks’ Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME).

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Description

Basal Medium Eagle (BME) in Hanks’ Buffer

Background

Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks’ (HBSS) salts for use without CO2.

Ingredients

As the composition, this media contains HBSS buffer

HBSS buffer contains:

  1. Potassium Chloride
  2. Potassium Phosphate, monobasic
  3. Sodium Bicarbonate
  4. Sodium Chloride
  5. And lastly, Sodium Phosphate, dibasic

Application

PurMa Biologics manufactures 117 types BME in HBSS buffer. This media has been successfully used for variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

Formulation

For complete formulation click, or alternatively  look at the “ Formulation Tab”:  BME with Hanks’ Buffer (HBSS) Formulation

References

  1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
  2. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
  3. Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007/BF0 2618060. PMID: 6187665.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

BME with Hanks’ Buffer (HBSS)

 

Quality Control

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    Advanced Iscove’s Modified Dulbecco’s Medium (A-IMDM)

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    • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
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    • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
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    Formulation

    Complete formulation is available here: Advanced Glasgow’s Formulation 

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    Williams' E Medium - Max

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    The advantages of Williams' E Medium - Max over Williams' E Medium are:

    • Higher PH Capacity
    • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
    • Possess longer shelf life.
    • More importantly, minimizes toxic ammonia build-up.
    • Significantly improves cell viability and growth.
    • Remains stable across a wide range of temperatures.
    • Improves the culture of primary cells.

    Mechanism

    Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

    Contents

    Williams' E Medium - Max contains:

    • 5 mM sodium pyruvate
    • High glucose (4.5g/L)
    • Non-Essential Amino Acids
    • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of William’s E Medium based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
    • Phenol Red
    Williams' E Medium - Max contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

    Formulation

    For complete formulation click here: Williams' E Medium-MAX Formulation

    References

    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • William’s E Medium

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    Williams′ Medium E

    Background

    Williams′ Medium E is a modification version of Williams′ Medium D by Williams and Gunn. Although, it is like MEM, but there is difference in glucose as well as amino acid contents.  This media was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been shown that this medium is suited for longer term adult primary cell culture.

    PurMa™ William's E Medium is

    • Suited for long term culture.
    • Enriched in amino acids.
    • Double the glucose compared to the original formulation.
    • Moreover, contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione as well as lipid methyl linoleate

    Moreover, PurMa™ William's E Medium also contains:

    • Low level of glucose (2.00 g/ liter). PurMa Biologics manufacturers the high glucose (4.5 g/L) version of this media as well (Cat #: P3S029880)
    • Although the standard formulation contains 2.2 g/L Sodium bicarbonate, but PurMa Biologics manufacturers the high sodium bicarbonate (3.7 g/L) Williams′ Medium E as well (Cat #: P3S032880)
    • 2 mM L-glutamine (292 mg/L)’
    PurMa Biologics manufactures 112 types of William's E Medium which is certainly the most comprehensive catalog of media. Why PurMa Biologics? Why we stand behind our cell culture media: Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable. Application of William's E Medium This media has elements supporting long term culturing of primary cells.. It utilizes a bicarbonate buffering system. This media has been used for primary as well as commercial cell lines including: primary liver epithelial cells, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes ,carcinomas as well as hybridoma cells. Formulation: The complete formulation is available in formulation tab as well here:William's E Medium Formulation

    References

    1. Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, Weisburger et al. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8.
    2. Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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