Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME). Currently, DMEM is one of the most widely used media. Several major modifications have been made to the original formulation:

  1. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Most of the DMEMs manufactured by various vendors including PurMa Biologics contain 4.5g/L glucose. Additionally, PurMa Biologics offers a (1.5 g/L) low glucose (P3SO28105) condition.
  2. It contains fourfold more amino acids and vitamins compared to the original formulation. Since then. It has made DMEM a good choice for growing the primary cell culture of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate.
  3. It has several fold more buffering capacity (3.7g/L NaHCO3)

DMEM Contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

DMEM contains:

  • High glucose (4.5g/L)
  • Non-essential amino acids
  • Sodium pyruvate (0.11 g/L)
  • Phenol red (0.0015g/L)
  • L-glutamine (0.585 g/L)

PurMa Biologics Manufactures three forms of DMEMs: Standard, MAX, and Advanced.  The optimization of these three forms of DMEM is the outcome of decades of cell culture research and experience.  Our DMEMs deliver exceptional growth and consistency for your cell culture projects.

Formulation: Complete formulation is available here: DMEM Formulation


  1. A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
  2. Effect of primary culture medium type for culture of canine fibroblasts on production of cloned dogs. Kim GA, Oh HJ, Kim MJ, Jo YK, Choi J, Kim JW, Lee TH, Lee BC. 2015 Sep 1;84(4):524-30. doi: 10.1016/j.theriogenology.2015.04.007. Epub 2015 Apr 18.PMID: 26001598
  3. Proliferation and gene expression of osteoblasts cultured in DMEM containing the ionic products of dicalcium silicate coating. Sun J, Li J, Liu X, Wei L, Wang G, Meng F.Biomed Pharmacother. 2009 Nov;63(9):650-7. doi: 10.1016/j.biopha.2009.01.007. Epub 2009 Feb 8.PMID: 19231130
  4. Medium [No authors listed]Curr Protoc Cell Biol. 2001 May;Appendix 2:Appendix 2B. doi: 10.1002/0471143030.cba02bs06.PMID: 18228282
  5. Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Ahmado A, Carr AJ, Vugler AA, Semo M, Gias C, Lawrence JM, Chen LL, Chen FK, Turowski P, da Cruz L, Coffey PJ.Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167/iovs.10-6374.PMID: 21743014
  6. Protective layer formation on magnesium in cell culture medium. Wagener V, Virtanen S.Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
  7. The viability change of pigskin in vitro. Ge L, Sun L, Chen J, Mao X, Kong Y, Xiong F, Wu J, Wei H. 2010 Jun;36(4):533-8. doi: 10.1016/j.burns.2009.08.001.PMID: 19836142
  8. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Soleimani M, Nadri S.Nat Protoc. 2009;4(1):102-6. doi: 10.1038/nprot.2008.221.PMID: 19131962
  9. Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests? Rohanová D , Boccaccini AR , Horkavcová D , Bozděchová P , Bezdička P , Častorálová M .J Mater Chem B. 2014 Aug 21;2(31):5068-5076. doi: 10.1039/c4tb00187g. Epub 2014 Jul 1.PMID: 32261840
  10. Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells. Wu X, Lin M, Li Y, Zhao X, Yan F. 2009 Mar;59(2):103-11. doi: 10.1007/s10616-009-9200-5. Epub 2009 Jun 4.PMID: 19496017
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature