PurMa Deoxynucleotide Triphosphates (dNTP) Mix - PurMabiologics

PurMa Deoxynucleotide Triphosphates (dNTP) Mix

$3.68$85.62

PurMaTM dNTP Mix, PCR Grade are ideal for a wide variety of molecular biology applications including all standard and sensitive PCR techniques. Also suitable for use with all PCR enzymes, including Taq DNA Polymerase, HotStarTaq DNA Polymerase, and reverse transcriptase enzymes.

Description

PurMaTM dNTP Mix, PCR Grade are ideal for a wide variety of molecular biology applications including all standard and sensitive PCR techniques. Also suitable for use with all PCR enzymes, including Taq DNA Polymerase, HotStarTaq DNA Polymerase, and reverse transcriptase enzymes. Strictly quality controlled PurMa dNTP mixs, contain equimolar solution of dATP, dCTP, dGTP and dTTP having greater than 99% purity, devoid of all contaminations of researcher’s concern. Designed for convenience and flexibility, to simplify your PCR reactions and to reduce risks of reaction set up errors. Standard nucleotides are available in different convenient sizes as an aqueous solution of total 40mM dNTP sodium salts in molecular biology grade water.

Application 

  • For various PCR amplifications

–non-specific DNA amplification

— Amplification for standard PCR

— for long range PCR (40kb)

— for multiplex PCR

— for high-fidelity PCR

  •  Two step RT-PCR
  • qPCR
  • Methylation-specific PCR
  • For reduction of primer dimers
  •  Substitute for dNTPs in any PCR reaction
  • Loop mediated isothermal amplification (LAMP)
  • Multiple displacement amplification (MDA)
  • cDNA synthesis
  • Genotyping
  • DNA sequencing
  • DNA labeling

 

 Qualitative parametersand Specification:

Parameter specification
Physical Appearance Clear, colorless solution
Concentration 10mM each nucleotide in water

 

PH value 7.3-7.5
Stability 36 months at -20C
Purity > 99% purity (Confirmed by HPLC)
 Tetra phosphates andPyrophosphate concentration < 0.003 pmol PPi/pmol dNTP
DNAse and RNAse activity Not detectable
Nicking activity Not detectable
Bacterial DNA contamination (E. coli DNA) Not detectable
Human DNA contamination (qPCR, human gDNA) Not detectable
Origin of synthesis Chemically synthesyzed

 

Product Highlights

  • High quality – >99% purity determined by HPLC
  • Long shelf-life – no need for aliquoting
  • Convenient formats – available in various concentrations, offering ease of use
  • Enzyme free – DNase, RNase and Nicking enzyme free
  • Free from inhibitors of qPCR, PCR and RT
  • Available in custom formulations and for commercial supply
  • Reduce pipetting steps minimize risk of contaminations
  • Suitable for all standard and highly sensitive PCR applications
  • Maximize consistency

 

Usage:     

PurMaTM dNTP Mix is a pre-mixed solution contains sodium salts of dATP, dCTP, dGTP and dTTP, each at 10mM in PurMaTM molecular biology grade water at pH 7.5. Thus the final concentration of the nucleotide mixture is 40mM (10 mM each). Use 1 μL of dNTP mix per 50 μL PCR reaction. Therefore, 1μl of the dNTP Mix in a 50μl reaction will give a final dNTP concentration of 200μM for each dNTP.

 

Quality assay:

The purity of nucleotides is crucial for the success of your PCR amplification processes since impurities of the building blocks of nucleic acids result in poor amplification sensitivity and quality of final product. PurMaTM dNTP Mixs are quality controlled to ensure high performance in most of the sensitive PCR techniques.

Using RT-PCR tested for absence of deoxyribonucleases, ribonucleases contamination, and nicking activities. The consistency of each lot of dNTP Mix is tested in a specific polymerase chain reaction requiring all four nucleotides.

Nucleotides are tested for free of dNTPs with modified bases, as well as tetra- and pyrophosphate contamination which act as inhibitor of PCR amplification reactions.

 

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