Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, 6 X 1000 ml

///Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, 6 X 1000 ml

Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12, 6 X 1000 ml

$201.00

Product Number: P3106-1000
Size: 6 X 1000 ml
Storage Temperature: 4°C
Category: Tissue Culture Media

Description

PurMaTM Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) provides superior quality, consistency and great control in mammalian cell culture. PurMaTM DMEM-F12 contains 1:1 mixture of DMEM and F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with F-12’s wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system lower than DMEM, therefore requires a 5–10% CO2 environment to maintain physiological pH. Experienced scientists at PurMabiologics have tested PurMa™ DMEM/F-12 and we believe it functions better than DMEM and IMDM on MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. PurMaTM DMEM-F12 contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

In general, DMEM: F-12 is more enriched but contains the following major changes compared to DMEM:

  • 2.5 mM L-glutamine
  • 15 mM HEPES
  • 0.5 mM sodium pyruvate

Documents

MSDS DMEM Nutrient mixture f-12- (Liquid)
DMEM F-12 Formulation

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