Eagle’s Minimal Essential Medium (EMEM)

Eagle’s Minimal Essential Medium (EMEM) is suitable for adhesive as well as suspension cell lines.  PurMa Biologics manufactures 106 types of this medium

Description

Eagle’s Minimum Essential Medium (EMEM)

Background

Eagle’s Minimal Essential Medium (EMEM) developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Ever since, several versions of EMEM have been developed by modification of its composition. This media has is suitable for a wide range of mammalian cells, adhesive as well as suspension cells.

Ingridients

Moreover, PurMa TM EMEM Medium contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (1.5 g/L)
  • Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
  • 2 mM L-glutamine (292 mg/L)
  • 1mM sodium pyruvate (0.11g/L)
  • Additionally, a higher concentration of amino acids, vitamins, inositol and choline are present in this medium.

PurMa Biologics manufactures 106 types of EMEM which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for EMEM media.

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.

We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.

We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.

Considering the highest quality, our prices are extremely reasonable.

Application of EMEM

Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cells lines. The dedicated scientists in PurMa Biologics have decades of experience with this media for culturing primary as well as commercial cell lines including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.

Formulation

for complete formulation click here:  EMEM Formultation 

References

  1. Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis. Hasday et al. J Immunol Methods. 1988 Nov 10;114(1-2):243-52. doi: 10.1016/0022-1759(88)90180-9. PMID: 3141516.
  2. Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis. Mignini et al. Chemioterapia. 1988 Feb;7(1):38-41. PMID: 3378274.
  3. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski et al. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID: 2461881.
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

EMEM Standard Formulation, EMEM w/o Glutamine, EMEM w/o Phenol Red, EMEM w/o Sodium Bicarbonate, EMEM w/o Sodium Pyruvate, EMEM w/o Glucose, EMEM w/o Glutamine, w/o Phenol Red, EMEM w/o Phenol Red, w/o Sodium Bicarbonate, EMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, EMEM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM High Glucose (4.5 g/L), EMEM High Sodium Bicarbonate (2.438 g/L), EMEM High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM 15 mM HEPES (3.6 g/L), EMEM 25 mM HEPES (5.9 g/L), EMEM 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, w/o Phenol Red, EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), EMEM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), EMEM w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), EMEM w/o Phenol Red, High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), EMEM w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

EMEM Formulation

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