F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium), 6 X 1000 ml

///F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium), 6 X 1000 ml

F-12K Medium (Kaighn’s Modification of Ham’s F-12 Medium), 6 X 1000 ml

$219.00

Product Number: P3108-1000
Size: 6 X 1000 ml
Storage Temperature: 4°C
Category: Tissue Culture Media

Description

Ham’s F-12K (Kaighn’s) Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) was originally used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a much lower serum environment.

Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine (therefore the media smells after incubated with cells after 48 hours), thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These compensated for supplemental serum. But based on our experience addition of 10 % FBS is necessary. PurMa TM F-12K Medium contains no proteins or growth factors and is therefore should be supplemented with FBS.  The FBS with this medium should be adjusted based on the cell lines. We have the best experience of this medium with various cells with about 10-15% of FBS. For cells such as Hek and COS-7 cells 7% FBS also gives proper and consistent results. Therefore, we strongly recommend a set of preliminary experiments with different FBS percentages.  And we recommend the lowest percentage of FBS as latter is variable and is a factor which may change the results.

PurMa TM F-12K Medium contains:

Low level of glucose (1.25 g/ liter)

Sodium bicarbonate (if you use higher than 5% CO2, higher % of sodium bicarbonate needs to be used)

2 mM L-glutamine

Documents

MSDS Kaighn’s Modification of Ham’s F-12 (Liquid)

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