Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX)

Kaighn's Modification of Ham's F-12-MAX (Ham's F-12K MAX)

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX)

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.  Ham’s F-12K MAX significantly improves cell viability and growth. We manufacture several types of Ham’s F-12K MAX based on your requirements.

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Description

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX)

Background

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of Ham’s F-12K MAX over Ham’s F-12K are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of Ham’s F-12K MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Ham’s F-12K MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here:PurMa™ Ham F12K-MAX Formulation

References

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  3. The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

HAM’S F-12K MAX Standard Formulation, HAM’S F-12K MAX w/o Phenol Red, HAM’S F-12K MAX w/o Sodium Bicarbonate, HAM’S F-12K MAX w/o Sodium Pyruvate, HAM’S F-12K MAX w/o Glucose, HAM’S F-12K MAX w/o Lipoic Acid, HAM’S F-12K MAX w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K MAX w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K MAX w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K MAX High Glucose (4.5 g/L), HAM’S F-12K MAX Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX High Sodium Bicarbonate (3.7 g/L), HAM’S F-12K MAX 15 mM HEPES (3.6 g/L), HAM’S F-12K MAX 25 mM HEPES (5.9 g/L), HAM’S F-12K MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, w/o Phenol Red, HAM’S F-12K MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), HAM’S F-12K MAX w/o Phenol Red, with 25 mM HEPES (5.9 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), HAM’S F-12K MAX w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), HAM’S F-12K MAX w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), HAM’S F-12K MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Phenol Red, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K MAX w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid, Powder
Size

1 X 10L ( Powder), 1 X 50 L (Powder), 10 X 1L EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml

Quality Control

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Formulation

PurMa™ Ham F12K-MAX Formulation

 

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