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PurMa
TM Advanced EMEM contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes. In Advanced EMEM, L‑alanine‑L‑glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced EMEM contains:
- All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- It includes the elements which significantly reduce the need for a serum for cell culture including:
Advanced EMEM is a substantial upgrade to the standard and MAX for of EMEM. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich
PurMaTM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
- PurMaTM Albumin: Chromatographically purified with IgG content <=0.1.0%.
- PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
In another word, advanced form of EMEM is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.
Chemically Defined EMEM (CD- EMEM) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD- EMEM soon will be offered globally to laboratories who are looking for consistency and repeatability among their data.
Formulation: Complete formulation is available here:
Advanced EMEM Formulation
References:
- Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
- Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
- Insulin-like growth factor binding protein-1 from Hep G2 cells is potently inhibited by the truncated IGF-I analogue des-(1-3) IGF-I. Lindgren BF, Isaksson M, Stern I, Hall K.Acta Endocrinol (Copenh). 1993 Jan;128(1):81-7. doi: 10.1530/acta.0.1280081.PMID:
- Extracellular ATP and ADA-buffer enable chick embryo fibroblasts to grow in secondary cultures in protein-free, hormone-free, extracellular growth factor-free medium. Pietrzkowski Z, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):143-52. PMID: 3197876.
- Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, Reiss K, Korohoda W. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID:
- Improved method for the isolation and cultivation of human scalp dermal papilla cells. Warren R, Chestnut MH, Wong TK, Otte TE, Lammers KM, Meili ML. J Invest Dermatol. 1992 May;98(5):693-9. doi: 10.1111/1523-1747.ep12499909. PMID:
- Human lens epithelial cell proliferation in a protein-free medium. Wormstone IM, Liu CS, Rakic JM, Marcantonio JM, Vrensen GF, Duncan G.Invest Ophthalmol Vis Sci. 1997 Feb;38(2):396-404.PMID:
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| Parameter |
Specification |
| Appearance |
Red, clear liquid |
| pH |
7.2 ± 0.1 |
| Osmolality |
275-360 mOsm/L |
| Endotoxin |
NMT< 2EU/mL |
| Mycoplasma |
Negative |
| Suitability |
Suitable for mammalian cell culture |
| Additive |
Sodium pyruvate |
| Indicator |
Phenol red |
| Mycoplasma Detection |
Negative |
| Sterility Tested |
Sterile filtered using 0.22 µm filter |
| Form |
Liquid |
| Shipping Condition |
Room temperature |
[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL
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