Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer

Minimum Essential Media-MAX (MEM-MAX) in Hanks’ Buffer

Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.   MEM-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of MEM-MAX based on your requirements.

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Description

Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer

Background

Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of MEM-MAX over MEM are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

Minimum Essential Media MAX (MEM-MAX) in Hanks’ Buffer contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

MEM-MAX in HBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For the complete formulation click here: MEM-MAX (HBSS) Formulation in Hanks’ Buffer

References

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
  3. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-MAX (HBSS) Standard Formulation, MEM-MAX (HBSS) w/o Phenol Red, MEM-MAX (HBSS) w/o Sodium Bicarbonate, MEM-MAX (HBSS) w/o Glucose, MEM-MAX (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, MEM-MAX (HBSS) 15 mM HEPES (3.6 g/L), MEM-MAX (HBSS) 25 mM HEPES (5.9 g/L), MEM-MAX (HBSS) Sodium Pyruvate (0.11 g/L), MEM-MAX (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-MAX (HBSS) High Glucose (4.5 g/L), MEM-MAX (HBSS) 15 mM HEPES (3.6 g/L), w/o Glucose, MEM-MAX (HBSS) 25 mM HEPES (5.9 g/L), w/o Glucose, MEM-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L)
Format

Liquid, Powder
Size

1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml

Quality Control

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Formulation

MEM-MAX (HBSS) in Hanks’ Buffer

SDS

SDS

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