Minimum Essential Media (MEM) in Earle’s Buffer
Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Description
Minimum Essential Media (MEM) in Earle’s Buffer
Background
Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
PurMa™ MEM media includes Earle’s Buffer
As the composition of Earle’s buffer, it contains:
- Calcium chloride
- Magnesium sulfate
- Potassium chloride
- Sodium bicarbonate
- Sodium chloride
- Sodium Phosphate, monobasic
PurMa Biologics manufactures 118 types of MEM, it also includes:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (2.2 g/L)
- 2 mM L-glutamine (292 mg/L)’
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices certainly are extremely reasonable.
Application:
MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle’s buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.
Formulation
For detailed formulation visit “the formulation Tab” as well as by clicking here: MEM Formulation in Earl’s Buffer
References
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 365437
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM Standard Formulation, MEM w/o Glutamine, MEM w/o Phenol Red, MEM w/o Sodium Bicarbonate, MEM w/o Glucose, MEM w/o Glutamine, w/o Phenol Red, MEM w/o Glutamine, w/o Sodium Bicarbonate, MEM w/o Phenol Red, w/o Sodium Bicarbonate, MEM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM 15 mM HEPES (3.6 g/L), MEM 25 mM HEPES (5.9 g/L), MEM Sodium Pyruvate (0.11 g/L), MEM High Glucose (4.5 g/L), MEM High Sodium Bicarbonate (3.7 g/L), MEM Low Sodium Bicarbonate (0.35 g/L), MEM Low Sodium Bicarbonate (0.85 g/L), MEM 15 mM HEPES (3.6 g/L), w/o Glucose, MEM 25 mM HEPES (5.9 g/L), w/o Glucose, MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
[vc-quality-control-tab]SDS
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