Minimum Essential Media (MEM) in Earle’s Buffer

Minimum Essential Media (MEM) in Earle’s Buffer was originally developed by Harry Eagle. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.

Description

Minimum Essential Media (MEM) in Earle’s Buffer

Background

Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2.

PurMa™ MEM media includes Earle’s Buffer

As the composition of Earle’s buffer, it contains:

  • Calcium chloride
  • Magnesium sulfate
  • Potassium chloride
  • Sodium bicarbonate
  • Sodium chloride
  • Sodium Phosphate, monobasic

PurMa Biologics manufactures 118 types of MEM, it also includes:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (2.2 g/L)
  • 2 mM L-glutamine (292 mg/L)’

Why PurMa Biologics? Why we stand behind our cell culture media:

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices certainly are extremely reasonable.

Application:

MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle’s buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.

Formulation

For detailed formulation visit “the formulation Tab” as well as by clicking here: MEM Formulation in Earl’s Buffer

References

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 365437

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM Standard Formulation, MEM w/o Glutamine, MEM w/o Phenol Red, MEM w/o Sodium Bicarbonate, MEM w/o Glucose, MEM w/o Glutamine, w/o Phenol Red, MEM w/o Glutamine, w/o Sodium Bicarbonate, MEM w/o Phenol Red, w/o Sodium Bicarbonate, MEM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM 15 mM HEPES (3.6 g/L), MEM 25 mM HEPES (5.9 g/L), MEM Sodium Pyruvate (0.11 g/L), MEM High Glucose (4.5 g/L), MEM High Sodium Bicarbonate (3.7 g/L), MEM Low Sodium Bicarbonate (0.35 g/L), MEM Low Sodium Bicarbonate (0.85 g/L), MEM 15 mM HEPES (3.6 g/L), w/o Glucose, MEM 25 mM HEPES (5.9 g/L), w/o Glucose, MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L), MEM 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

MEM in Earl’s Buffer Formulation

 

SDS

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