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IMDM MAX:
In general, the advantages of IMDM-MAX over IMDM are as follows:
- Higher PH Capacity
- Contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life)
- Minimizes toxic ammonia build-up.
- Improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Minimizes the growth of pathogens by stabilizing the PH.
- Improves the culture of primary cells.
Additionally, IMDM-MAX contains:
- 1.0 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.024 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of IMDM -MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
L-glutamine is a vital amino acid that is supplemented with mammalian cell culture media. This important amino acid has several important functions including the production of purine and pyrimidine nucleotides amino sugars, and glutathione as well as performing as the secondary source of energy. The latter is more crucial in cells dividing rapidly. Generally, this amino acid is used in cell culture media in the range of 2–6 mM.
One drawback of using L-glutamine is that in solutions such as cell culture media, L-glutamine degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. The speed of degradation of L-glutamine depends on time, temperature, and pH. One way to minimize the degradation of L-glutamine degradation in media is to gradually add these amino acids to your cell culture but has to be calculated to keep the level of L-glutamine at a constant level. Practically, this strategy even though is possible but tedious, and depending on the volume of cell culture work in your la, keeping track of time to keep the level of L-glutamine at the same level is difficult and could be tedious if not impossible.
A viable and well-proven alternative for using L-glutamine, which is less sensitive to environmental factors such as pH, is applying L-alanyl-L-glutamine, which is more stable in aqueous solutions and does not easily degrade and cells gradually release aminopeptidases that hydrolyze the dipeptide, slowly releasing L-alanine and L-glutamine into the culture media. The resulting L-glutamine and L-alanine are then used by the cells for protein production or in the TCA cycle. PurMa
TM GluaSup (Cat# P3S210 559) is a proprietary combination of L-alanyl-L-glutamine and other elements which prevent degradation of L-glutamine in the period when is released from L-alanyl-L-glutamine before being utilized by cells. Using PurMa
TM GluaSup maximizes energy metabolism and high growth yield. The other components in PurMa
TM GluaSup neutralize the trace of ammonia on your cells whose generation in cell culture is inevitable in presence of L-glutamine and harmful to the cells.
Additionally, PurMa
TM GluaSup offers more buffering capacity which maintains physiological pH more efficiently. An extra buffering system in this media is a powerful tool to avoid changing the PH which results in instability in growing and cellular mechanisms. Changing the PH is also one of the most common factors for growing mold and other pathogens. IMDM-MAX functions better than regular IMDM on primary cell culture and most of the commercial cell lines such as MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
IMDM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS. In general,
Formulation: Complete formulation of IMDM-MAX, Click here:
IMDM-MAX Formulation
References:
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
- Improved Survival and Initiation of Differentiation of Human Induced Pluripotent Stem Cells to Hepatocyte-Like Cells upon Culture in William’s E Medium followed by Hepatocyte Differentiation Inducer Treatment, Minoru Tomizawa, Fuminobu Shinozaki, Yasufumi Motoyoshi, Takao Sugiyama, Shigenori Yamamoto, Naoki Ishige, PLoS One. 2016; 11(4): e0153435. Published online 2016 Apr 13. doi: 10.1371/journal.pone.0153435, PMCID: PMC4830564
- Cell culture medium formulation and its implications in cancer metabolism,Tobias Ackermann, Saverio Tardito, Trends Cancer. Author manuscript; available in PMC 2019 Jun 10. Published in final edited form as: Trends Cancer. 2019 Jun 4; 5(6): 329–332. Published online 2019 May 29. doi: 10.1016/j.trecan.2019.05.004, PMCID: PMC6557711
- Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080/0032472031000141250. PMID: 19746589
- Essential components for ex vivo proliferation of mesenchymal stromal cells. Fekete N, Rojewski MT, Lotfi R, Schrezenmeier H. Tissue Eng Part C Methods. 2014 Feb;20(2):129-39. doi: 10.1089/ten.TEC.2013.0061. Epub 2013 Jul 5. PMID: 23713576
- Effects of glutamine supply on growth and metabolism of mammalian cells in chemostat culture. Vriezen N, Romein B, Luyben KC, van Dijken JP. Biotechnol Bioeng. 1997 May 5;54(3):272-86. doi: 10.1002/(SICI)1097-0290(19970505)54:3<272::AID-BIT8>3.0.CO;2-C. PMID: 18634093.
- Development of a serum-free medium for the production of humanized antibody from Chinese hamster ovary cells using a statistical design. Kim EJ, Kim NS, Lee GM. In Vitro Cell Dev Biol Anim. 1998 Nov-Dec;34(10):757-61. doi: 10.1007/s11626-998-0029-6.
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Parameter |
Specification |
Appearance |
Red, clear liquid |
pH |
7.2 ± 0.1 |
Osmolality |
275-360 mOsm/L |
Endotoxin |
NMT< 2EU/mL |
Mycoplasma |
Negative |
Suitability |
Suitable for mammalian cell culture |
Additive |
Sodium pyruvate |
Indicator |
Phenol red |
Mycoplasma Detection |
Negative |
Sterility Tested |
Sterile filtered using 0.22 µm filter |
Form |
Liquid |
Shipping Condition |
Room temperature |
[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed, 1 x 500 mL, 6 x 500 mL, 1 x 1000 mL, 6 x 1000 mL
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