Minimum Essential Media (MEM) In Hanks’ Buffer
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
Description
Minimum Essential Media (MEM) In Hanks’ Buffer
Background
Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.
PurMa™ MEM media in Hank’s buffer includes Hank’s buffer
Hanks buffer, it contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM in Hanks’ Buffer contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
- 2 mM L-glutamine (292 mg/L)
Moreover, PurMa Biologics manufactures 122 types of MEM.
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.
Applications
MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).
Formulation
Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks’ Buffer
References
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM (HBSS) Standard Formulation, MEM (HBSS) w/o Glutamine, MEM (HBSS) w/o Phenol Red, MEM (HBSS) w/o Sodium Bicarbonate, MEM (HBSS) w/o Glucose, MEM (HBSS) w/o Glutamine, w/o Phenol Red, MEM (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, MEM (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM (HBSS) 15 mM HEPES (3.6 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), MEM (HBSS) Sodium Pyruvate (0.11 g/L), MEM (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) High Glucose (4.5 g/L), MEM (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), w/o Glucose, MEM (HBSS) 25 mM HEPES (5.9 g/L), w/o Glucose, MEM (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
[vc-quality-control-tab]SDS
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