Minimum Essential Media (MEM) In Hanks’ Buffer

Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle. This media includes higher concentrations of amino acids making it suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.

Description

Minimum Essential Media (MEM) In Hanks’ Buffer

Background

Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific  requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks’ salts for use without CO2.

PurMa MEM media in Hank’s buffer includes Hank’s buffer

Hanks buffer, it contains:

  1. Potassium Chloride
  2. Potassium Phosphate, monobasic
  3. Sodium Bicarbonate
  4. Sodium Chloride
  5. Sodium Phosphate, dibasic

Additionally, MEM in Hanks’ Buffer contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
  • 2 mM L-glutamine (292 mg/L)

Moreover, PurMa Biologics manufactures 122 types of MEM.

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Applications

MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).

Formulation

Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks’ Buffer

References

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM (HBSS) Standard Formulation, MEM (HBSS) w/o Glutamine, MEM (HBSS) w/o Phenol Red, MEM (HBSS) w/o Sodium Bicarbonate, MEM (HBSS) w/o Glucose, MEM (HBSS) w/o Glutamine, w/o Phenol Red, MEM (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, MEM (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM (HBSS) 15 mM HEPES (3.6 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), MEM (HBSS) Sodium Pyruvate (0.11 g/L), MEM (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) High Glucose (4.5 g/L), MEM (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), w/o Glucose, MEM (HBSS) 25 mM HEPES (5.9 g/L), w/o Glucose, MEM (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), MEM (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

MEM (HBSS) in Hanks’ Buffer

 

SDS

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