PurMa 2X Master Mix - PurMabiologics

PurMa 2X Master Mix

$8.29$275.26

PurMaTM 2X Master Mix is pre-mixed ready-to-use 2X concentrated solution that is optimized for a wide variety of PCR applications for consistent and efficient PCR amplification.

SKU: N/A Category:

Description

PurMaTM 2X Master Mix is pre-mixed ready-to-use 2X concentrated solution that is optimized for a wide variety of PCR applications for consistent and efficient PCR amplification. The solution contains bacterially derived Taq DNA polymerase, dNTPs (dATP, dGTP dCTP, dTTP), MgCl2, 10X Green dye (composed of blue and yellow dye) and other components except DNA templets and primers at optimal concentrations. Two different non-interfering tracking dyes and precipitant that added into the PurMaTM Master Mix maintain sufficient density for direct loading PCR products onto agarose gels. The dyes also allow monitoring of progress of DNA migration during electrophoresis. This pre-mixed formulation not only saves time by simplifying the assembly of PCR reaction but also decreases the risk of contamination and pipetting errors as the number of pipetting steps reduced.

PurMaTM 2X Master Mix is compatible with common PCR additives like DMSO. These additives do not affect migration of the dyes or change the color of master mix.

As PCR performance is not interfered with the dyes, PurMaTM 2X Master Mix is compatible with PCR based downstream applications like DNA sequencing, but the master mix is not recommended for those downstream applications used absorbance or fluorescence excitation, as the blue and yellow tracking dyes included in the mixture may interfere with those application processes.

PurMaTM 2X Master mix contains:

  • PCR Mix
    — Taq DNA polymerase (0.05 U/µL)
    — 10X green loading dye
    — 4 mM MgCl2
    — 0.4 mM of each dNTP
  • PurMa water
    — Nuclease-free water

 

Specifications of PurMaTM 2X Master mix:

*Simplify assembly, convenience
*Optimized for highly efficient and reproducible PCR
*Highly thermostable (half-life is more than 40 min at 95°C)
*Greater consistency
*Higher processivity
*More sensitive and specific
*Minimal contamination risk
*Minimal pipetting error
*Low cost

To set up a 25 ul PCR reaction, simply add 12.5 ul of PurMaTM 2X Master Mix to specific primers, DNA template, and the nuclease free water that is included to make the volume. No additional loading buffer is required in the whole process.

Aliquot to avoid repeated freezing and thawing.

 

Applications of the PurMaTM 2X  Master Mix:

* Routine PCR amplification of genomic, viral and plasmid templates.
* Used for RT-PCR, qPCR
* High throughput PCR
* DNA cloning and subcloning.
* Primer extension.
* Genotyping
*Genetic profiling
* DNA Labelling
*Gene expression pathway analysis
* DNA mutation detection
*Characterization of genetically modified organisms
* Generating hybridization probes for Southern or Northern blot.

Documents

1) Master Mix Data Sheet
2) MSDS

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Additional information

Size

, ,

Reactions

20X 100RN, 20X RN, 2X 100 RN

Title

Go to Top