PurMa™ Phosphatase Inhibitor Cocktail (100X), 2X 300µl

////PurMa™ Phosphatase Inhibitor Cocktail (100X), 2X 300µl

PurMa™ Phosphatase Inhibitor Cocktail (100X), 2X 300µl

$59.00

Product Code: P6104-02
Size: 2 X 300µl
Storage Condition: -20°
Category: Biochemical Reagents

Description

Phosphatases are important enzymes that are involved in a variety of biochemical pathways in living cells, such as regulating signal transduction events in eukaryotic cells. In intact cells, inhibitors or compartmentalization regulate the phosphatase activities to maintain the proper function of signaling pathways. However, during cell lysis, the controlled cellular environment is disrupted leading to phosphatases to become unregulated. The common result of this unregulated state is representation of meaningless protein activities such as phosphorylation status. So, when studying cellular processes involving protein phosphorylation (i.e. signaling pathway, protein-protein interactions, or analysis of phosphorylated sites on a protein and phosphoprotein purification), it is crucial to inhibit cellular protein phosphatases by phosphatase inhibitors. PurMa™ phosphatase inhibitor cocktail has been successfully tested and proven to inhibitsinhibit a wide range of phosphatases for more than two decades in several laboratories around the world. It is well proven to work well in common detergents such as Triton, 1% SDS and NP-40. The PurMa™ phosphatase inhibitor (100X) is provided as a ready-to-use broad-spectrum solution of inhibitors targeting both serine/threonine and protein tyrosine phosphatases (PTPs) and is suitable for use with any cell or tissue extract.

Features and Benefits:
• Economical: More cost effective than other similar commercially available products for equivalent volumes
• Easy-to-use: The kit comes with two vials each contains 250 µl of 100X. It has 50 % glycerol so freezing and thawing wouldn’t be a problem
• Complete protection: Instantly protect your proteins against a broad range of proteases.
• Activity Assay for the proteases after adding PurMaTM Protease inhibitor cocktail

No Classes of Protease Phosphatase Inhibitor Concentration
1 Serine / Threonine and acidic phosphatases Sodium fluoride(NaF) 10 mM
2 Serine / Threonine phosphatases beta-Glycerophosphate 10mM
3 Serine / Threonine phosphatases Sodium Pyrophosphate 10 mM
4 Phosphotyrosyl phosphatases (PTPs) Sodium Metavanadate (NaNO3) 1 mM
5 Serine phosphatases PMSF 1 mM
6 Metalloprotease EDTA/ EGTA 10mM
7 Tyrosine phosphatase and alkaline phosphatase I Sodium Orthovanadate (Na3VO4) 10 µM
8 Acid phosphatases Sodium Molybdate (Na2MoO4) 10 µM

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