Williams' E Medium - Max

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Williams' E Medium - Max

Williams’ E Medium – Max

$43.82 – $826.46

Williams’ E Medium – Max contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life while significantly improving cell viability and growth. We manufacture several types of Williams’ E Medium based on your requirements.

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Description

Williams’ E Medium – Max

Background

Williams’ E Medium – Max is a commonly used media for Mammalian cell culture.

Advantages

The advantages of Williams’ E Medium – Max over Williams’ E Medium are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Williams’ E Medium – Max contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of William’s E Medium based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Williams’ E Medium – Max contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation click here: Williams’ E Medium-MAX Formulation

References

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

William E-MAX Formulation

Quality Control

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    Background

    Leibovitz’s L-15 Medium - MAX is one of the commonly used Media for Mammalian cell culture.

    Advantages

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    Contents

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    Formulation

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    References

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    Background

    PurMaTM Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-EMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

    Contents

    Advanced EMEM Contains:

    • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
    • A-EMEM incorporates elements that significantly reduce the need for a serum for cell culture.

    Crucial Ingredients:

    • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
    • PurMaTM Insulin Recombinant
    • Trace of other elements which mimic the elements in fetal bovine serum.
    Consequently, Advanced Eagle’s Minimal Essential Medium (A-EMEM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced EMEM (CD-A-EMEM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

    Formulation

    Complete formulation is available here: Advanced EMEM Formultaion

    References

    1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
    2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Minimum Essential Media (MEM) In Hanks’ Buffer

    Background

    Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific  requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks' salts for use without CO2.

    PurMa™ MEM media in Hank’s buffer includes Hank's buffer

    Hanks buffer, it contains:
    1. Potassium Chloride
    2. Potassium Phosphate, monobasic
    3. Sodium Bicarbonate
    4. Sodium Chloride
    5. Sodium Phosphate, dibasic

    Additionally, MEM in Hanks’ Buffer contains:

    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
    • 2 mM L-glutamine (292 mg/L)
    Moreover, PurMa Biologics manufactures 122 types of MEM.

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

    Applications

    MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).

    Formulation

    Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks' Buffer

    References

    1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
    2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

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    Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

    Background

    Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer is a commonly used media for Mammalian cell culture.

    Advantages

    The advantages of BME-MAX over BME are:

    • Higher PH Capacity
    • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
    • Possess longer shelf life.
    • More importantly, minimizes toxic ammonia build-up.
    • Significantly improves cell viability and growth.
    • Remains stable across a wide range of temperatures.
    • Improves the culture of primary cells.

    Mechanism

    Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents degradation of L-glutamine.

    Contents

    Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer contains:

    • 5 mM sodium pyruvate
    • High glucose (4.5g/L)
    • Non-Essential Amino Acids
    • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of BME-MAX in EBBS based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
    • Phenol Red
    BME-MAX in EBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

    Formulation

    The complete formulation can be found here: BME-MAX with Earl's Buffer Formulation

    References

    1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al.  Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
    2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
    3. An improved culture system for secondary palatal elevation. Lewis CA, et al. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
    [/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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