Although Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating cell lines, it is certainly quite suitable for slow-growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore making it an excellent choice for adhesive cells.

scove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, high-density cell lines. Therefore, it is excellent for Jurkat, Hek, COS-7, and macrophage cells. IMDM is a modified and more advanced version of DMEM. Additionally, it includes selenium as well as additional amino acids and vitamins. it is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore it makes it excellent for adhesive cells.

Ingredients

Iscove’s Modified Dulbecco’s Medium (IMDM) contains:

• High Buffering Capacity with High NaHCO3 as well as High HEPES
• Sodium Bicarbonate (3.02 g/L)
• 5.9 g/L HEPES
• High glucose 4.5g/L
• Non-Essential Amino Acids
• 110 mg/L Sodium pyruvate (1.0 mM)
• 15 mg/L Phenol Red
• 0.584 g/L L-glutamine

Formulation

Complete formulation of IMDM is available on our website as well as here: IMDM Formulation 

References

1. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
2. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195