Cell Culture Media2023-11-17T05:36:29-07:00

A Comprehensive Collection of Cell Culture Media

DMEM

Dulbecco’s
Modified
Eagle’s Medium

DMEM/F12

Dulbecco’s
Modified Eagle’s Medium
Nutrient Mixture F-12

HAM’S -F12

Kaighn’s
Modification of
Ham’s F-12 Medium

RPMI 1640

Roswell Park
Memorial Institute 1640

IMDM

Iscove’s
Modified
Dulbecco’s Medium

EMEM

Eagle’s
Minimal Essential
Medium

McCoy’s

McCoy’s 5A
Modified Media

Williams’

Williams’
Medium E

Leibowitz’s

Leibovitz’s
L-15
Medium

Glasgow’s

Glasgow’s
Modified Eagle’s Medium
Glutamine-Free

MEM

Minimum Essential Media
in
Earl’s Buffer

MEM-α

Minimum Essential Media
Alpha
in Earl’s Buffer

MEM-α-N

Minimum Essential Media
Alpha with Nucleosides
in Earl’s Buffer

BME

Basal Medium Eagle
in
Earl’s Buffer

MEM

Minimum Essential Media
in
Hanks’ Buffer

MEM-α

Minimum Essential Media
Alpha
in Hanks’ Buffer

MEM-α-N

Minimum Essential Media
Alpha with Nucleosides
in Hanks’ Buffer

BME

Basal Medium Eagle
in
Hanks’ Buffer

Cell Culture Media

PurMa™ Cell Culture media are the most commonly used, and well described media for growing various commercial as well as primary cell lines. Moreover, the components are US made and high quality. We particularly use amino acids with 99.6% purity to make our media. Further, this, as well applies to other ingredients used to make our  media. Our products, definitely,  provides all the necessary nutrients to support optimal cell growth and function.

Our Mission

PurMa Biologics has 27 years of experience in producing  media  as well as conducting  biochemical experience in highest possible levels. We manufactures over 2300 various types and  media for use in numerous cell culture applications. All the media are made in the state of  art facility in Oklahoma and we are not dependent on international transportation. Additionally, Cell culture process  is not just about growing a cell line. We manufacture our media in a way to have almost full consistency and reproducibility among various Lots.

Quality Control

Finally, We vigorously screen for mycoplasma and other common pathogens such as bacteria, mold/Yeast and viruses. but , however, mycoplasma contamination should be taken seriously as it deteriorate the cells gradually and certainly, causes inconsistency among different experiments.

Reference

Basic Techniques in Mammalian Cell Tissue Culture. Phelan et al. Curr Protoc Toxicol. 2016 Nov 1;70:A.3B.1-A.3B.22. doi: 10.1002/cptx.13.

What Is Cell Culture?2023-11-17T04:32:03-07:00

Cell as well as tissue culture is the process by which cells are grown under controlled circumstances. Cell culture is performed outside of their natural environment. Tissue culture, depending on the tissues, could be called different names. For example, tissue culture for retina, called whole mount renal culture. 

cells of interest are isolated from living tissue; they can subsequently be maintained under controlled conditions. Most of the cells need to be incubated at 37 °C in a CO2 incubator.  The specific criteria surrounding the condition in which the cells are incubated vary for each cell type, but generally includes of a appropriate container with a proper medium that supplies the main elements including: amino acids, carbohydrates, vitamins, minerals as well as growth factors, hormones. It is crucial to maintain the level of pH and CO2/ O2).

What Is Insulin And Why Is It Used In Cell Culture Media?2023-11-17T04:32:51-07:00

Insulin has several main functions in cell culture:

  1. Facilitating glucose uptake.
  2. Enhancing cell growth.
  3. Speeding the cell cycle.

Insulin is commonly used in serum-free and chemically defined media. PurMa Biologics uses this component in various media.

Why Do We Need Sodium Bicarbonate In Our Media And How Does It Maintain Buffering Capacity (The Ph) In Relation With Co2 In Cell Culture Medium?2023-11-17T04:33:16-07:00

The optimal cell growth process outside the body requires special conditions. This environment is provided in a CO2 incubator. The CO2 incubator must provide the factors that affect cell growth and keep them constant. These elements consist of temperature of 37°C ± 0.5°C, 5% carbon dioxide, and 95% humidity must be met. This causes the pH to be around 7 which is the best pH for cell growth. There is a possibility to have various buffering system but carbonate buffer system, HCO3- and H2CO3 has the least affect on growth of cells. In this condition, H2CO3 in water is in equilibrium with H2O+CO2. To keep the CO2 in the buffer solution at the desired level we must supply CO2 in the incubator, otherwise all the CO2 would dissolve and the H2CO3 would disappear, and the buffering system will be diminished. So, if you use a CO2 incubator, NaHCO3must be used in the media. The concentration of the sodium bicarbonate in the medium must correlate with the level of CO2 in the atmosphere incubator.

What type of cell culture media do not require using a CO2 incubator?2023-11-17T04:33:03-07:00

Media without sodium bicarbonate do not require CO2 such as MEM with Hank’s buffer.

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