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November 19, 2024
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PurMa Biologics
Manufacturing More Than 3000 Cell Culture Products.
PurMa Biologics has the Most Comprehensive Collection of Cell Culture Reagents.
Experience
Currently, with over 27 years of experience in cell culture science, PurMa Biologics offers an extensive range
of more than 3000 high-quality reagents, not to mention Mycoplasma Detection Elements.
Additionally, our commitment to quality means that we produce the highest number of
Cell and Tissue Culture Reagents.
of more than 3000 high-quality reagents, not to mention Mycoplasma Detection Elements.
Additionally, our commitment to quality means that we produce the highest number of
Cell and Tissue Culture Reagents.
Cell Culture Reagents manufactured by PurMa Biologics
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+
Cell culture media by PurMa Biologics
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+
Years experience in cell culture
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+
years experience in protein biochemistry
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+
Frequently Asked Question
Frequently Asked Questions
We believe in a future where renewable energy sources play avital role in reducing carbon emissions and creating a sustain- able planet.
Not all the adhesive cell lines can become suspension cells. But however, cell lines such as Hek 293 cells and Hela cells can be used to make a suspension cell line. The main reason for that would be to generate more surface for application such as transfection for plasmids hard to enter the cells or escape lysosomal degradation. In this case with much more available penetrating plasmid, the chance of reaching nuclei will get significantly higher. With adhesive cells, there is always some portion of cells floating. You can keep them floating by shaking them several times / days.
For two main reasons:
- Serum is the source of inconsistency and variability. Each batch of serum is essentially different than another batch.
- Serums are the source of early stages of some pathogens such as micrococcus. The small structure of these elements causes them passing through 0.22 µm. They adhere to flask and dishes and after about one week, they turn into mycoplasma.
- The color of the media has NOT changed, and the media won’t be turbid. Whereas, with bacterial, mold, fungus contamination, you could clearly see the contamination.
- The deteriorating impact is gradual and the change in functionality usually is overlooked.
- the most important damage of mycoplasma to your laboratory is compromising reproducibility and reproductivity. So, if another lab performs the exact study and is free of mycoplasma, the result will be significantly different.
Endotoxins or lipopolysaccharides are the toxins released into the environment around the gram-negative bacteria caused by compromising or death of these organelles. Gram negative endotoxins are the most significant pyrogen which causes fever in humans and causes severe contamination in cell culture. Due to the wide spread of these types of bacteria, endotoxin is present almost everywhere. Among them is in the digestive system and the blood stream. The latter could cause severe infection with a variety of symptoms such as fever, hypotension, nausea, shivering and shock. Endotoxins in vitro affect the results and compromise the conclusions.
In cell culture the acceptable level of endotoxin is 0.5 units/ml which only achieved by complete attention and awareness of the nature and causes of endotoxin contamination. As sterile conditions are not always provided, endotoxin contamination during in vitro work is very common. The most common source of endotoxin is water since distillation and deionizing columns do not remove endotoxin. In in vitro studies, endotoxins may be contained in buffers and/or on materials and affect observed results. the presence of relatively high levels of environmental endotoxins (100 EU/ml) has a dramatic effect on the results. That is because they generate wear debris in vitro and have confirmed the significant effect of environmental endotoxin contamination on cytokines which is one of the main players of intracellular signaling in cell culture process and directly affect the nuclear phosphorylation of CREB.
There is various type of endotoxins ranging from molecular weights of 10,000 to 1,000,000. However, since proteins are in the same molecular weight range as endotoxins, usual sterilization such as filtration cannot eliminate the endotoxins from the mixture of proteins. That brings us to the conclusion that preventing endotoxin contamination by purchasing chemicals, cell culture reagent and water from a reputable vendor is still the best strategy to fight endotoxins. As endotoxins cause the proteins to have negative charge in most solutions, ion exchange chromatography is an option to decontaminate protein from these contaminants.
It thoroughly depends on the cell lines. The heat inactivation of FBS is unnecessary for most of the cell lines. In has been proven to us that the differentiated cell line do much better in heat inactivated serum.
The best way to find out whether heat inactivation is necessary for your cell culture is to empirically determine that in your own study.
mycoplasma and certain viruses are essentially non-infectious after heat-inactivation. But whether it is needed or not , you must keep the experiments consistent and do it for all your experiments as still a lot is unknown about FBS contents.
37°C is the temperature of the tissue which the cells coming from in normal conditions. So, we try to imitate the temperature where the cells have come from. 5% CO2 is to maintain the pH of the media and correlates with the amount of NaHCO3 in your media.
The PurMa Biologics Commitment
Our Amazing Products
We work with unparallel professionalism and constantly dedicated to providing a consistent and diverse cell culture media available. Over the years, we have garnered the trust of our clients because of our
impeccable quality products & values leading to long-term relationships and continued association.Working with an unwavering commitment,we have been able to fulfil customer expectations. Please follow us on Linkedin about our latest news as well as new products: Linkedin.
Roswell Park Memorial Institute (RPMI) 1640 Medium
medium, meticulously formulated to support an extensive range of cell culture applications. This term is derived from the renowned Roswell Park Memorial Institute, and facilitates optimal cell growth for thriving in the supporting environment.
Hanks Balanced Salt Solution (HBSS)
Discover our HBSS, with availability in standard and concentrated formulations. The Hanks balanced salt solution composition balances the HBSS for the maintenance of cellular homeostasis and feasibility according to various procedures.
Cell Culture Media Components
PurMa Biologics generates three major type of this powerful media: Regular Williams′ Medium E, Williams′ Medium E containing L- Alanine L- Glutamine, as well as Advanced Williams′ Medium E which needs only a small amount of FBS. PurMa Biologics by generating 162 different types of Williams′ Medium E is the leading company in manufacturing the highest quality of this sophisticated media.
Trypsin
Cell culture provides ideal enzymatic activity for smooth facilitation and efficient dissociation and passage. Believe in the trypsin that augurs seamless subculturing and proper maintenance of the cell lines. Cell culture-suited Trypsin is a standard method to detach adherent cell culture and monolayers.
McCoy's 5A Medium
One should explore a broader range of McCoy’s 5 A medium, an ideal versatile formulation that speeds up the growth of cell types. No matter whether you are working with adherent or suspension cultures, it provides all the nutrients for robust cell proliferation.
PurMa Biologics Laboratory Service Provider
Get Essential Reagents for Your Laboratory Needs
PurMa Biologics is home to the most comprehensive collection of cell culture reagents. First, and foremost, our commitment to quality is unparalleled. Above all, we are dedicated to producing the most consistent and diverse cell culture media available. Furthermore, at PurMa Biologics we value our customers and strive to build long-term relationships, especially with those who share our values and trust in our products.
Quality Support- All our products undergo stringent quality tests to control the measures and ensure trustworthiness and consistency giving optimal confidence in all research outcomes.
Expert Guidance- You should always bank on things with technical assistance and guidance. All our dedicated professionals address your queries to optimize cell culture protocols.
Customer Satisfaction- We give extreme priority to customer satisfaction. We strive forward to exceed expectations by delivering exceptional products & personalized service tailored to your needs.
Promising Best Quality Services
Quality
First and foremost, at PurMa Biologics, we are committed to producing the most comprehensive collection of high-quality
cell culture reagents. Our products are unmatched in terms of reproducibility as well as consistency, therefore
making PurMa Biologics a leading manufacturer in the field.
cell culture reagents. Our products are unmatched in terms of reproducibility as well as consistency, therefore
making PurMa Biologics a leading manufacturer in the field.
Cell Culture Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Transfection Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Fetal Bovine Horse & Serums
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Trypsin
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Antibiotic Mixes
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell and Tissue culture Suited Buffers
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Mycoplasma Detection and Elimination Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cryoprotective Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Water and Other Water Treatments
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Transfection Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Fetal Bovine Horse & Serums
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Trypsin
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Antibiotic Mixes
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell and Tissue culture Suited Buffers
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Mycoplasma Detection and Elimination Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cryoprotective Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Water and Other Water Treatments
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Transfection Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Fetal Bovine Horse & Serums
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Trypsin
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Antibiotic Mixes
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell and Tissue culture Suited Buffers
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Mycoplasma Detection and Elimination Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cryoprotective Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Water and Other Water Treatments
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Transfection Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Fetal Bovine Horse & Serums
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Includes 90 Parameters
Trypsin
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Antibiotic Mixes
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell and Tissue culture Suited Buffers
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Mycoplasma Detection and Elimination Reagents
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cryoprotective Media
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Cell Culture Suited Water and Other Water Treatments
Allergy testing can be useful at different stages of life, like discovering that a family member has a condition.
Experience
Currently, with over 27 years of experience in cell culture science, PurMa Biologics offers an extensive range
of more than 3000 high-quality reagents, not to mention Mycoplasma Detection Elements.
Additionally, our commitment to quality means that we produce the highest number of
Cell and Tissue Culture Reagents.
of more than 3000 high-quality reagents, not to mention Mycoplasma Detection Elements.
Additionally, our commitment to quality means that we produce the highest number of
Cell and Tissue Culture Reagents.
Year of business
0
+
Number of cell culture reagents manfactured by PurMa Biologics
0
+
Years experience in cell culture
0
+
years experience in protein biochemistry
0
+
Promising Best Quality Services
The PurMa Biologics Commitment
Finally, our commitment to quality is unparalleled, and consequently, we’re dedicated to providing the most consistent and diverse cell culture media available. Undoubtedly, we value our customers and strive to build long-term relationships with those who share our values and trust in our products. Moreover, at PurMa Biologics, we prioritize customer satisfaction and aim to create a supportive environment for everyone. Furthermore, our commitment to our customers is unwavering, and accordingly, we will continue to go the extra mile to ensure their satisfaction.
Please follow us on Linkedin about our latest news as well as new products: Linkedin
Frequently Asked Question
Frequently Asked Questions
We believe in a future where renewable energy sources play avital role in reducing carbon emissions and creating a sustain- able planet.
Not all the adhesive cell lines can become suspension cells. But however, cell lines such as Hek 293 cells and Hela cells can be used to make a suspension cell line. The main reason for that would be to generate more surface for application such as transfection for plasmids hard to enter the cells or escape lysosomal degradation. In this case with much more available penetrating plasmid, the chance of reaching nuclei will get significantly higher. With adhesive cells, there is always some portion of cells floating. You can keep them floating by shaking them several times / days.
For two main reasons:
- Serum is the source of inconsistency and variability. Each batch of serum is essentially different than another batch.
- Serums are the source of early stages of some pathogens such as micrococcus. The small structure of these elements causes them passing through 0.22 µm. They adhere to flask and dishes and after about one week, they turn into mycoplasma.
- The color of the media has NOT changed, and the media won’t be turbid. Whereas, with bacterial, mold, fungus contamination, you could clearly see the contamination.
- The deteriorating impact is gradual and the change in functionality usually is overlooked.
- the most important damage of mycoplasma to your laboratory is compromising reproducibility and reproductivity. So, if another lab performs the exact study and is free of mycoplasma, the result will be significantly different.
Endotoxins or lipopolysaccharides are the toxins released into the environment around the gram-negative bacteria caused by compromising or death of these organelles. Gram negative endotoxins are the most significant pyrogen which causes fever in humans and causes severe contamination in cell culture. Due to the wide spread of these types of bacteria, endotoxin is present almost everywhere. Among them is in the digestive system and the blood stream. The latter could cause severe infection with a variety of symptoms such as fever, hypotension, nausea, shivering and shock. Endotoxins in vitro affect the results and compromise the conclusions.
In cell culture the acceptable level of endotoxin is 0.5 units/ml which only achieved by complete attention and awareness of the nature and causes of endotoxin contamination. As sterile conditions are not always provided, endotoxin contamination during in vitro work is very common. The most common source of endotoxin is water since distillation and deionizing columns do not remove endotoxin. In in vitro studies, endotoxins may be contained in buffers and/or on materials and affect observed results. the presence of relatively high levels of environmental endotoxins (100 EU/ml) has a dramatic effect on the results. That is because they generate wear debris in vitro and have confirmed the significant effect of environmental endotoxin contamination on cytokines which is one of the main players of intracellular signaling in cell culture process and directly affect the nuclear phosphorylation of CREB.
There is various type of endotoxins ranging from molecular weights of 10,000 to 1,000,000. However, since proteins are in the same molecular weight range as endotoxins, usual sterilization such as filtration cannot eliminate the endotoxins from the mixture of proteins. That brings us to the conclusion that preventing endotoxin contamination by purchasing chemicals, cell culture reagent and water from a reputable vendor is still the best strategy to fight endotoxins. As endotoxins cause the proteins to have negative charge in most solutions, ion exchange chromatography is an option to decontaminate protein from these contaminants.
It thoroughly depends on the cell lines. The heat inactivation of FBS is unnecessary for most of the cell lines. In has been proven to us that the differentiated cell line do much better in heat inactivated serum.
The best way to find out whether heat inactivation is necessary for your cell culture is to empirically determine that in your own study.
mycoplasma and certain viruses are essentially non-infectious after heat-inactivation. But whether it is needed or not , you must keep the experiments consistent and do it for all your experiments as still a lot is unknown about FBS contents.
37°C is the temperature of the tissue which the cells coming from in normal conditions. So, we try to imitate the temperature where the cells have come from. 5% CO2 is to maintain the pH of the media and correlates with the amount of NaHCO3 in your media.
The Products We Offer are Exceptional
We operate with unmatched professionalism and are committed to offering the most comprehensive catalog of mammalian cell culture reagents. We also offer a wide range of consistent and diverse cell culture media. Our exceptional products and values have earned our customers’ trust, resulting in lasting partnerships and ongoing connections. By maintaining a steadfast dedication, we have met and exceeded customer demands. Please Connect with us on LinkedIn, Facebook, and Instagram for updates on our latest news and new products. Below are some examples of each category of products.
Please visit our website for the complete list of reagents: https://purmabiologics.com/.
PurMa Biologics manufactures the most comprehensive Cell Culture Cell Culture Media
PurMa Biologics produces three main kinds of this potent medium: The regular form of each meida,′ the form containing L—Alanine L—Glutamine instead of L- Glutamine, and the Advanced form of media requiring minimal FBS (2-5%). PurMa Biologics is the top company producing the best quality of conventional cell culture media by creating 1600 various types:
https://purmabiologics.com/cell-culture-media/
For example:
- Roswell Park Memorial Institute 1640 (RPMI 1640) Medium
- McCoy’s 5A Medium
- Hanks Balanced Salt Solution (HBSS)
PurMa Biologics Laboratory Service Provider
PurMa Biologics has the most extensive array of cell culture reagents available. Above all, our commitment to excellence is unmatched. We are committed to creating the most reliable and varied cell culture media on the market. In addition, at PurMa Biologics, we prioritize our customers and aim to establish enduring connections, particularly with individuals who embrace our values and have faith in our products.
- Quality Control (QC): Our products are rigorously tested to ensure reliability and consistency, providing high confidence in research results.
- Professional advice: Always rely on technical support and guidance. Cell culture experts are here to answer your questions and improve cell culture procedures.
Guaranteeing the quality
At PurMa Biologics, our primary goal is to create the most extensive range of top-notch products, including substances for culturing cells. Our products are unparalleled regarding reproducibility and consistency, hence Establishing PurMa Biologics as a top producer in the industry.Experience
At present, PurMa Biologics provides a wide variety of products in cell culture science, backed by 27 years of experience. Containing over 3000 premium reagents, in addition to Mycoplasma Detection Elements. Furthermore, our cell culture technique’s dedication to excellence results in us generating the greatest quantity of Reagents for Cell and Tissue Culture.What good works we are doing
Our Latest Research
We have world class pathologists & Lab assistants. We are equipped with best
laboratory machinery & reagents. We ensure best quality findings.
laboratory machinery & reagents. We ensure best quality findings.
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REVIEWS
cheryl Terska
2023-04-27
So much easier and less invasive than previous covid tests. Staff was friendly , prompt and caring.
Mona Nikoo
2023-03-31
Great customer service, the best thing is to use saliva for Covid tests, and is not harmful like the other ones also got results fast.
Layla Green
2023-03-06
So helpful and nice and also gave me all the details and I had result within 2 hours it’s also a saliva test and he let me do it myself!
Keon Azadi
2023-02-24
My mom and I had a really good experience with the PurMa Biologcs they use saliva for covid testing instead of sticking that cutip up your brain, they had the result in one hour before we knew it!
karimovi
2023-02-16
One of the worst companies and the worst manager ever, products with no quality or organized work, no COVID testing at all it's fake, a messy place which I don't recommend!
B Hippocampus
2022-07-07
One of the worst customer services experiences I have encountered. Yikes! Never received ordered product either...
niyaf alkadhem
2022-02-27
Great customer service, communication ,fast item quotes with good offers every time.
Reagents, media and kits as good as Fisher and stemcell technology but with faster delivery .
Jackson Nehrt
2019-10-09
Beautiful place
