1. Through peptide synthesis:
We have extended experience in protein expression of bacterial and mammalian cells. Optimization of the requested proteins is usually done within 2-3 business days. PurMabiologics uses various methods to optimize the expression of the proteins, such as western blot, ELISA, and activity assays. The protein of interest can be constructed with or without tags, which will be used for protein purification. Following expression, we can purify your proteins with several techniques, such as affinity columns and column chromatography, including size exclusion chromatography (gel filtration) and ion exchange chromatography. To get a reasonable quote, please contact us.