Leibovitz's L-15 Medium - MAX

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Leibovitz's L-15 - MAX

Leibovitz’s L-15 Medium – MAX

$37.11 – $736.19

Leibovitz’s L-15 Medium – MAX significantly improves the viability and growth of cells.  It additionally contains L-Alanyl-L-Glutamine and therefore supplies a longer shelf life.  Moreover, we manufacture several types of Leibovitz’s L-15 MAX based on your requirements.

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Description

Leibovitz’s L-15 Medium – MAX

Background

Leibovitz’s L-15 Medium – MAX is one of the commonly used Media for Mammalian cell culture.

Advantages

The advantages of Leibovitz’s L-15 MAX over Leibovitz’s L-15 are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. Therefore, one way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture.  Unfortunately, maintaining a consistent level of L-glutamine by tracking time can be a tedious and potentially impossible task.

Alternatively, L-alanyl-L-glutamine can be used since it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

Leibovitz’s L-15 Medium – MAX contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of Leibovitz’s L-15 MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Leibovitz’s L-15 MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation is here: Leibovitz’s L-15 MAX Formulation

References

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

Leibovitz’s L-15 MAX

 

Quality Control

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    References

    1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
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    3. An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
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    Formulation

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    References

    1. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
    2. Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
    3. In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Minimum Essential Media (MEM) in Earle’s Buffer

    Background

    Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

    PurMa™ MEM media includes Earle's Buffer

    As the composition of Earle's buffer, it contains:
    • Calcium chloride
    • Magnesium sulfate
    • Potassium chloride
    • Sodium bicarbonate
    • Sodium chloride
    • Sodium Phosphate, monobasic

    PurMa Biologics manufactures 118 types of MEM, it also includes:

    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (2.2 g/L)
    • 2 mM L-glutamine (292 mg/L)’

    Why PurMa Biologics? Why we stand behind our cell culture media:

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices certainly are extremely reasonable.

    Application:

    MEM has been used for cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. Additionally, MEM in Earle's buffer uses a sodium bicarbonate buffer system (2.2 g/L) that requires a 5–10% CO2 environment to maintain physiological pH.

    Formulation

    For detailed formulation visit "the formulation Tab" as well as by clicking here: MEM Formulation in Earl's Buffer

    References

    1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
    2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 365437
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • Advanced Williams’ E Medium

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Advanced Williams’ E Medium

    Background

    PurMaTM Advanced Williams’ E Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Williams’ E, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

    Contents

    Advanced Williams’ E Contains:

    • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
    • A-Williams’ E incorporates elements that significantly reduce the need for a serum for cell culture.

    Crucial Ingredients:

    • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
    • PurMaTM Insulin Recombinant
    • Trace of other elements which mimic the elements in fetal bovine serum.
    Consequently, Advanced Williams’ E Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Williams’ E (CD-A-Williams’ E) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

    Formulation

    Complete formulation is available: Advanced Williams' E Medium Formulation 

    References

    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive Sodium pyruvate
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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