Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

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Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

Background

PurMa^TM Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-BME, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Consequently, Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced BME in Earle's Buffer

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer

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Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer

Background

PurMa^TM Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced MEM-α. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Consequently, Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α (CD-A-MEM-α) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM-alpha in Earle's Buffer

References

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, Wun CC, Grunert GM. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Roudebush WE, Often NL, Butler WJ. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Advanced MEM-Alpha with Nucleosides in Earle’s Buffer

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Advanced-MEM-Alpha with Nucleosides in Earle’s Buffer

Background

PurMa^TM Advanced-MEM-Alpha with Nucleosides (A-MEM-α-N) in Earle’s Buffer contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-MEM-α-N, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Consequently, Advanced-MEM-Alpha with Nucleosides (A-MEM-α-N) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α-N (CD-A-MEM-α-N) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM alpha with Nucleoside in Earls Buffer

References

The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Roudebush WE, et al. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Advanced Minimum Essential Media (A-MEM) in EBSS

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Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer

Background

PurMa^TM Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in A-MEM. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Consequently, Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM (CD-A-MEM) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM Formulation in Earle's Buffer

References

Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, Byard R, Dardick I, Tsang BK. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Basal Medium Eagle (BME) in Earle’s Buffer

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Basal Medium Eagle (BME) in Earle’s Buffer

Background

Basal Medium Eagle (BME) in Earle’s Buffer originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle’s as well as in Hanks' salts for use without CO₂.

Ingredients

PurMa™ BME media in Earle's buffer contains:

Earl's buffer includes:

Calcium chloride
Magnesium sulfate
Potassium chloride
Sodium bicarbonate
Sodium chloride
and lastly, Sodium Phosphate, monobasic

PurMa Biologics so far, manufactures 109 types BME in Earle’s buffer.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. undoubtly, considering the highest quality, our prices certainly are extremely reasonable.

Application

BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.

Formulation

for complete formulation click here, alternatively you can find it in “Formulation Tab”: BME with Earle's Buffer Formulation References:

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

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Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: BME-MAX with Earl's Buffer Formulation

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
An improved culture system for secondary palatal elevation. Lewis CA, et al. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

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Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

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Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Formulation

The complete formulation can be found here: BME-MAX with Hanks' Buffer (HBSS) Formulation

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

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Dulbecco’s Phosphate Buffered Saline (DPBS)

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Dulbecco's Phosphate Buffered Saline (DPBS)

Background

Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution. It is particularly used to maintain viability of cells for a short time instead of promoting their growth. During interval period we maintain osmotic balance and physiological pH to protect cellular integrity. So, DPBS serves as an isotonic saline solution or buffer for washing cells and tissues like in chromatographic procedures where incubation is not necessary.

Grades of Phosphate Buffered Saline (PBS)

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101) we finally sterilize the buffers by autoclaving the solution and and packding in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. we finally eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

General Feature

PurMa Biologics manufactures this buffer in two pH: 7.4 ± 0.01 and 7.2 ± 0.01 at 25°C.
We rigorously sterilize it by 0.22µm membrane.
Additionally, it is a cost-effective balanced salt solution used for a variety of biochemical applications.
PurMa Biologics packs all our buffer under N_2,as a result, the pH wont change over the time before opening the bottle.

Specific Feature

Cell Culture Suited
We deplete Mg²⁺ and Ca²⁺ ions
Our manufacturing process removes RNAse /DNase
Free of Proteases.
PurMa Biologics rigorously test for contaminating, nonspecific endonuclease, exonuclease, as well as RNase activity.

For complete formulation, Click here:DPBS Formulation References:

Plaque formation and isolation of pure lines with poliomyelitis viruses. Dulbecco et al.J Exp Med.; 1954 Jan 31 99(2): 167–182. doi: 10.1084/jem.99.2.167. PMID: 13130792.
Deep freezing of sheep embryos. Willadsen Set al. J Reprod Fertil. 1976 Jan;46(1):151-4. doi: 10.1530/jrf.0.0460151. PMID: 1271336.

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.01; 7.4 ± 0.01; 7.6 ± 0.01
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

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Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

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Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) over DMEM:

Higher PH Capacity Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore: Possess longer shelf life. More importantly, minimizes toxic ammonia build-up. Significantly improves cell viability and growth. Remains stable across a wide range of temperatures. Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation:

For complete formulation Click here: DMEM MAX Formulation

References:

A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Ahmado A, et al., Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167/iovs.10-6374.PMID: 21743014
Protective layer formation on magnesium in cell culture medium. Wagener V, et al., Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

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Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

DMEM F-12 MAX over DMEM:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains:
5 mM sodium pyruvate
High glucose (4.5g/L)
Non-Essential Amino Acids
3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM F12-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
Phenol Red

DMEM F12-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation:

Complete formulation is here: DMEM F-12 MAX Formulation

References:

Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:18

[/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

[fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" 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Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

Background

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of EMEM-MAX over EMEM are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: EMEM-MAX Formulation

References

Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, et al. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, et al. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, et al. Histochem Cytobiol. 1988;26(3):123-31. PMID:

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Earle’s Balanced Salt Solution (EBSS)

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Dulbecco's Phosphate Buffered Saline (DPBS)

Background

Earle's Balanced Salt Solution (EBSS) is an isotonic buffer solution. It is however, contains inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment. The solution additionally provides an ideal environment to preserve the structural and physiological integrity of cells invitro by maintaining pH as well as the balance between intra and extracellular osmotic pressure.

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101) we finally sterilize the buffers by autoclaving the solution and and packding in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102) In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics produces the most sophisticated endotoxin removal. For that we pass the buffer through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. we finally eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

General Features

It is cost effective.
Cell Culture Suited
This buffers maintains the cell integrity of cells or tissues during irrigation, transportation, or dilution.
Provides an consistent environment during the washing and re-suspend cells
PurMa Biologics offers EBSS in three pH formats, pH 7.2, 7.4, and pH 7.6.

Specific Features

Glucose maintains the intra and extracellular osmotic pressure as well as provides the principal source of energy for cellular metabolism.
EBSS Provides water and important inorganic ions necessary for cellular metabolism.
This buffer Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6)
PurMa Biologics rigorously depletes Mg²⁺ and Ca ²⁺ from the buffers
Free of RNAse /DNase
Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
It is rigorously tested for contamination of endotoxin and mycoplasma.
Is manufactured in three pH: 7.2 ± 0.01, pH: 7.4 ± 0.01, and 7.6 ± 0.01 at 25°C.
Sterile filtered by 0.22µm membrane (in case of endotoxin-depleted EBSS by 0.1µm)
As it is packed under N_2,the pH won’t change over time before opening the bottle.

Application

EBSS is especially has an excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.

Formulation

For complete formulation, click here: EBSS Formulation References

Recovery from potentially lethal damage induced by spirohydantoin mustard on 9L cells in vitro. Bertrand M, Deen DF, Hoshino T, Knebel K. Cancer Treat Rep. 1980 Aug-Sep;64(8-9):889-95.
Rotavirus survival in conventionally treated drinking water. Sattar SA, Raphael RA, Springthorpe VS. Can J Microbiol. 1984 May;30(5):653-6. doi: 10.1139/m84-097.
Glucose elevates ornithine decarboxylase expression in Vero cells. Lundgren DW, Prokay SL. J Cell Physiol. 1988 Dec;137(3):469-75. doi: 10.1002/jcp.1041370311.

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1; 7.4 ± 0.1; 7.6 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon Read more

Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

Advanced Basal Medium Eagle (A-BME) in Earle’s Buffer

Background

Contents

Advanced BME in EBSS Contains:

Crucial Ingredients:

Formulation

References

Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer

Background

Contents

Advanced MEM-α in EBSS Contains:

Crucial Ingredients:

Formulation

References

Advanced-MEM-Alpha with Nucleosides in Earle’s Buffer

Background

Contents

Advanced MEM-α-N in EBSS Contains:

Crucial Ingredients:

Formulation

References

Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer

Background

Contents

Advanced MEM in EBBS Contains:

Crucial Ingredients:

Formulation

References

Basal Medium Eagle (BME) in Earle’s Buffer

Background

Ingredients

PurMa™ BME media in Earle's buffer contains:

Earl's buffer includes:

Why PurMa Biologics? Why we stand behind our cell culture media.

Application

Formulation

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Background

Advantages

The advantages of BME-MAX over BME are:

Mechanism

Contents

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer contains:

Formulation

References

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

Background

Advantages

The advantages of BME-MAX over BME are:

Mechanism

Contents

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains:

Formulation

References

Dulbecco's Phosphate Buffered Saline (DPBS)

Background

Grades of Phosphate Buffered Saline (PBS)

Regular Grade Buffer

Cell Culture Suited Grade Buffer

RNase and DNase Free Grade Buffer

Cell Culture Suited / Endotoxin Depleted Grade Buffer

The Endotoxin Removing Procedure from Buffer

General Feature

Specific Feature

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

Background:

Advantages:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) over DMEM:

Mechanism:

Contents:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) contains:

Formulation:

References:

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Background:

Advantages:

DMEM F-12 MAX over DMEM:

Mechanism:

Contents: