asal Medium Eagle (BME) in Hanks’ Buffer
Background
Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks’ (HBSS) salts for use without CO2.
Ingredients
As the composition, this media contains HBSS buffer
HBSS buffer contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- And lastly, Sodium Phosphate, dibasic
Application
PurMa Biologics manufactures 117 types of BME in HBSS buffer. This media has been successfully used for a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.
Formulation
For the complete formulation of Basal Medium Eagle (BME) in Hanks’ Buffer click here : BME with Hanks’ Buffer (HBSS) Formulation
References
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962.
-
- Basal Medium Eagle (BME) in Hanks’ Buffer, Cell Culture Media
Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer
- Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as serum-reducing ingredients. Additionally, Advanced BME needs 2-5% FBS and it furthermore provides more consistency.
- Read more
-
- Basal Medium Eagle (BME) in Hanks’ Buffer, Cell Culture Media
Basal Medium Eagle (BME) in Hanks’ Buffer
- Basal Medium Eagle (BME) in Hanks’ Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME).
- Read more
-
- Basal Medium Eagle (BME) in Hanks’ Buffer, Cell Culture Media
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer
- Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. BME-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.
- Read more
