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Basal Medium Eagle (BME) in Hanks’ Buffer
Basal Medium Eagle (BME) in Hanks’ Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME).
Description
Background
Basal Medium Eagle (BME) in Hanks’ Buffer was originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks’ (HBSS) salts for use without CO2.
Ingredients
As the composition, this media contains HBSS buffer
HBSS buffer contains:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- And lastly, Sodium Phosphate, dibasic
Application
PurMa Biologics manufactures 117 types of Basal Medium Eagle (BME) in Hanks’ Buffer. This media has been successfully used for a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as primary rat astrocytes.
Formulation
For complete formulation click, or alternatively look at the “ Formulation Tab”: BME with Hanks’ Buffer (HBSS) Formulation
References
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
- Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007/BF0 2618060. PMID: 6187665.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | N/A |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | BME (HBSS) Standard Formulation, BME (HBSS) w/o Glutamine, BME (HBSS) w/o Phenol Red, BME (HBSS) w/o Sodium Bicarbonate, BME (HBSS) w/o Glucose, BME (HBSS) w/o Glutamine, w/o Phenol Red, BME (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, BME (HBSS) 15 mM HEPES (3.6 g/L), BME (HBSS) 25 mM HEPES (5.9 g/L), BME (HBSS) With Sodium Pyruvate (0.11 g/L), BME (HBSS) High Sodium Bicarbonate (0.85 g/L), BME (HBSS) High Glucose (4.5 g/L), BME (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), BME (HBSS) 25 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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Frequently asked questions
How should I store Basal Medium Eagle (BME) in Hanks’ Buffer?
Store at 2 to 8 degrees C, protected from light, and do not freeze unless the label states otherwise. Freezing can precipitate salts and degrade light sensitive components such as riboflavin and folic acid. Always follow the storage line on the Certificate of Analysis for the specific lot.
What is the shelf life once opened?
Unopened and correctly stored, the typical shelf life is 12 months from manufacture. Once opened, use within about four weeks and always handle in a laminar flow hood, since the practical limit after opening is contamination risk rather than chemical stability.
Is it sterility and endotoxin tested?
Yes. Every lot is sterile filtered and tested for sterility and endotoxin, and a Certificate of Analysis is issued for each lot. The COA is available on request for any catalogue number.
Do I need to add L-glutamine?
It depends on the formulation you order. Standard formulations may contain free L-glutamine, which degrades in solution over time, while MAX formulations use a stable L-alanyl-L-glutamine dipeptide that does not require fresh supplementation. Check the option you have selected before adding more.
Can I order this in bulk or as a custom formulation?
Yes. Bulk volumes and custom formulations are made to order, including adjusted glucose, bicarbonate, buffering and cation content. Use the request bulk pricing button on this page and include the specification you need.
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