Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

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Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

$40.40 – $775.35

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.   BME-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.

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Description

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of BME-MAX in HBBS based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

BME-MAX in HBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: BME-MAX with Hanks’ Buffer (HBSS) Formulation

References

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
  3. An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

BME-MAX with Hanks’ Buffer (HBSS)

 

Quality Control

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    Background

    Eagle's Minimal Essential Medium (EMEM) developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Ever since, several versions of EMEM have been developed by modification of its composition. This media has is suitable for a wide range of mammalian cells, adhesive as well as suspension cells.

    Ingridients

    Moreover, PurMa TM EMEM Medium contains:
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    • Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
    • 2 mM L-glutamine (292 mg/L)
    • 1mM sodium pyruvate (0.11g/L)
    • Additionally, a higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
    PurMa Biologics manufactures 106 types of EMEM which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for EMEM media.

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. Considering the highest quality, our prices are extremely reasonable.

    Application of EMEM

    Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cells lines. The dedicated scientists in PurMa Biologics have decades of experience with this media for culturing primary as well as commercial cell lines including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.

    Formulation

    for complete formulation click here:  EMEM Formultation  References
    1. Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis. Hasday et al. J Immunol Methods. 1988 Nov 10;114(1-2):243-52. doi: 10.1016/0022-1759(88)90180-9. PMID: 3141516.
    2. Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis. Mignini et al. Chemioterapia. 1988 Feb;7(1):38-41. PMID: 3378274.
    3. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski et al. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID: 2461881.
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    Background

    Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) is a commonly used media for Mammalian cell culture.

    Advantages

    The advantages of IMDM-MAX over IMDM are:

    • Higher PH Capacity
    • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
    • Possess longer shelf life.
    • More importantly, minimizes toxic ammonia build-up.
    • Significantly improves cell viability and growth.
    • Remains stable across a wide range of temperatures.
    • Improves the culture of primary cells.

    Mechanism

    Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

    Contents

    Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) contains:

    • 5 mM sodium pyruvate
    • High glucose (4.5g/L)
    • Non-Essential Amino Acids
    • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of IMDM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
    • Phenol Red
    IMDM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

    Formulation

    Complete formulation click here: IMDM-MAX Formulation 

    References

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    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
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link_hover_color="" border_sizes="" border_sizes_top="" border_sizes_right="" border_sizes_bottom="" border_sizes_left="" border_color="" border_style="solid" border_radius_top_left="" border_radius_top_right="" border_radius_bottom_right="" border_radius_bottom_left="" box_shadow="no" box_shadow_vertical="" box_shadow_horizontal="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index="" overflow="" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" skip_lazy_load="" background_position="center center" background_repeat="no-repeat" fade="no" background_parallax="none" enable_mobile="no" parallax_speed="0.3" background_blend_mode="none" video_mp4="" video_webm="" video_ogv="" video_url="" video_aspect_ratio="16:9" video_loop="yes" video_mute="yes" video_preview_image="" pattern_bg="none" 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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Basal Medium Eagle (BME) in Earle’s Buffer

    Background

    Basal Medium Eagle (BME) in Earle’s Buffer originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle’s as well as in Hanks' salts for use without CO2.

    Ingredients

    PurMa™ BME media in Earle's buffer contains:

    Earl's buffer includes:

    1. Calcium chloride
    2. Magnesium sulfate
    3. Potassium chloride
    4. Sodium bicarbonate
    5. Sodium chloride
    6. and lastly, Sodium Phosphate, monobasic
    PurMa Biologics so far, manufactures 109 types BME in Earle’s buffer.

    Why PurMa Biologics? Why we stand behind our cell culture media.

    Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. undoubtly, considering the highest quality, our prices certainly are extremely reasonable.

    Application

    BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

    Formulation

    for complete formulation click here, alternatively you can find it in “Formulation Tab”:  BME with Earle's Buffer Formulation References:
    1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
    2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • McCoy’s 5A Modified Media (M5AMM)

    [fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    McCoy’s 5A Modified Media (M5AMM)

    Background

    McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the and the full control you need for mammalian cell culture. MCCOY’S 5A Contains:
    • High glucose (3g/L)
    • Non-Essential Amino Acids
    • Phenol Red
    • L-glutamine

    Why PurMa Biologics? Why we stand behind our cell culture media:

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

    Application

    McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas  Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

    Formulation

    complete formulation is available in formulation tab as well as here:McCoy's 5A Formulation

    References

    1. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
    2. Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
    3. In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH  7.2 ± 0.1
    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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