Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

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Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: BME-MAX with Earl's Buffer Formulation

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
An improved culture system for secondary palatal elevation. Lewis CA, et al. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

[/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

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Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer

Background

Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.

Advantages

The advantages of BME-MAX over BME are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Formulation

The complete formulation can be found here: BME-MAX with Hanks' Buffer (HBSS) Formulation

References

Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	N/A
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

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Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) over DMEM:

Higher PH Capacity Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore: Possess longer shelf life. More importantly, minimizes toxic ammonia build-up. Significantly improves cell viability and growth. Remains stable across a wide range of temperatures. Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation:

For complete formulation Click here: DMEM MAX Formulation

References:

A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Ahmado A, et al., Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167/iovs.10-6374.PMID: 21743014
Protective layer formation on magnesium in cell culture medium. Wagener V, et al., Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228

[/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

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Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

DMEM F-12 MAX over DMEM:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains:
5 mM sodium pyruvate
High glucose (4.5g/L)
Non-Essential Amino Acids
3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM F12-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
Phenol Red

DMEM F12-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation:

Complete formulation is here: DMEM F-12 MAX Formulation

References:

Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:18

[/fusion_text][fusion_table fusion_table_type="1" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

[fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

Background

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of EMEM-MAX over EMEM are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: EMEM-MAX Formulation

References

Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, et al. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, et al. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, et al. Histochem Cytobiol. 1988;26(3):123-31. PMID:

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Eukaryotic Cell Culture Suited Antibiotics

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box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Eukaryotic Cell Culture Suited Antibiotics

Background

PurMa Biologics offers Eukaryotic Cell Culture Suited Antibiotics mix. Moreover, we manufacture a wide collection of antibiotic types. Additionally, our Cell Culture Suited Antibiotics offer protection for specific cell lines (see below). Notably, The main function of each is summarized in the table below and the full description and complete mechanism of action can be found in the mentioned literature, Click here: PurMa Antibiotics Booklet for Eucaryotes

Specific Criteria

It does not contain any PH-manipulating ingredients
After preparation, we sterilize the antibiotic mix by passing it through 0.1 µm filters.
We Pack our antibiotic mix under N2

Furthermore, we manufacture PurMa™ Cell Culture Suited Antibiotic Mixes in PurMa™ Ultra-Pure Cell Culture Suited Water . For more details click here: PurMa™ Ultra-Pure Cell Culture Suited water

Guideline

Formulations and preparation guidelines, click here: PurMa Antibiotics Guidelines for Mammalian Cells_Eukaryotes

References

Efficient selection for high-expression transfectants with a novel eukaryotic vector. Niwa H, Yamamura K, Miyazaki J. Gene. 1991 Dec 15;108(2):193-9. doi: 10.1016/0378-1119(91)90434-d. PMID: 1660837.
Neomycin Sulfate Improves the Antimicrobial Activity of Mupirocin-Based Antibacterial Ointments. Blanchard C, Brooks L, Beckley A, Colquhoun J, Dewhurst S, Dunman PM. Antimicrob Agents Chemother. 2015 Nov 23;60(2):862-72. doi: 10.1128/AAC.02083-15. Print 2016 Feb. PMID: 26596945.

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset="" /][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]20 ml, 50 ml, 100 ml Read more

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX)

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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX)

Background

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of IMDM-MAX over IMDM are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

Complete formulation click here: IMDM-MAX Formulation

References

Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080/0032472031000141250. PMID: 19746589

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX)

[fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX)

Background

Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of Ham’s F-12K MAX over Ham’s F-12K are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here:PurMa™ Ham F12K-MAX Formulation

References

Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Leibovitz’s L-15 Medium – MAX

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pattern_custom_bg="" pattern_bg_color="" pattern_bg_style="default" pattern_bg_opacity="100" pattern_bg_size="" pattern_bg_blend_mode="normal" mask_bg="none" mask_custom_bg="" mask_bg_color="" mask_bg_accent_color="" mask_bg_style="default" mask_bg_opacity="100" mask_bg_transform="left" mask_bg_blend_mode="normal" render_logics="" absolute="off" absolute_devices="small,medium,large" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_background_color="" sticky_height="" sticky_offset="" sticky_transition_offset="0" scroll_offset="0" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" 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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" 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Leibovitz’s L-15 Medium - MAX

Background

Leibovitz’s L-15 Medium - MAX is one of the commonly used Media for Mammalian cell culture.

Advantages

The advantages of Leibovitz’s L-15 MAX over Leibovitz’s L-15 are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. Therefore, one way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. Unfortunately, maintaining a consistent level of L-glutamine by tracking time can be a tedious and potentially impossible task. Alternatively, L-alanyl-L-glutamine can be used since it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Formulation

The complete formulation is here: Leibovitz's L-15 MAX Formulation

References

The effects of insulin, transferrin and androgens on rat prostate explants in serum-free organ culture. Nguyen-Le XK, Brière N, Corcos J. 1997;6(3):339-49. doi: 10.1002/biof.5520060304. PMID: 9288404.
Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière N, Droz D.Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Mammalian Cryoprotective Media (MCM-D) with DMSO

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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Mammalian Cryoprotective Media (MCM-D)

Background

Mammalian Cryoprotective Media (MCM-D) with DMSO provides the best cryoprotection with proven 69% recovery rate (see below). Exposing cells to temperatures below 0°C without the aid of cryoprotectants typically cause cell death. During cryopreservation cooling aqueous solutions below the equilibrium freezing point inevitably result in the formation of ice crystals in the extracellular media and a decrease in the concentration of water which has two key consequences:

Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.

More Importantly, we always manufacture our cryoprotective media using our most sophisticated water , for more details, Click here: PurMa™ Ultra-Pure Cell Culture Suited Water PurMa Biologics is the leading company in manufacturing cryoprotective media. We follow two essential goals:

Allowing cells to be stored at deep cryogenic temperatures with minimal cell damage and recovery with high levels of appropriate functionality and viability, and
Allow minimal toxicity. Undoubtedly, this is a challenge we have overcome in the past 3 decades.

Accordingly, we manufacture several DMSO -dependent and DMSO Free cryoprotective media. PurMa^TM (MCM) contains DMSO. PurMa^TM Mammalian Cryoprotective Media (MCM) has an excellent performance for short-term cold storage as well as long-term cryopreservation. Please see the below figure

DMSO benefits cryoprotection and cryoprotective agent in several ways

Provides strong molecular stability.
Besides, facilitates a high non-conductor constant.
It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
Especially, DMSO makes a hydrogen bond with water which is 30% stronger than between two water molecules.
The proprietary formulation of PurMa^TM Mammalian Cryoprotective Media (MCM) allows the MCM to get inside the cells instantly and lower cell death because of penetration/freezing.

Special Features

Ready-to-Use medium.
Each Lot of our Mammalian Cryoprotective Media (MCM-D) with DMSO goes through pathogens and sterility evaluations.
We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.
PurMa^TM MCM-D results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 69% survival and recovery rate after long term cryopreservation.
Sterile (Passed through 0.2µm filters).
Moreover, PurMa^TM MCM-D provides an exceptional cryopreservation for primary and commercial cell lines
Suitable for clinical applications.

Guideline

For PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

The chemical and physical properties of DMSO. MacGregor WS. Ann N Y Acad Sci. 1967 Mar 15;141(1):3-12. doi: 10.1111/j.1749-6632.1967.tb34860.x. PMID: 5342259.
Polyampholytes as cryoprotective agents for mammalian cell cryopreservation. Matsumura et al. Cell Transplant. 2010;19(6):691-9. doi: 10.3727/096368910X508780. Epub 2010 Jun 3. PMID: 20525437.

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red liquid
pH	7.4 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Sodium pyruvate	NA
Indicator	NA
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Dry Ice

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 10 ml, 1 x 100 ml Read more

McCoy’s 5A Modified Media (M5AMM)

[fusion_builder_container type="flex" hundred_percent="no" hundred_percent_height="no" min_height_medium="" min_height_small="" min_height="" hundr... 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filter_sepia_hover="0" filter_opacity_hover="100" filter_blur_hover="0"][fusion_builder_row][fusion_builder_column type="1_1" layout="1_1" align_self="auto" content_layout="column" align_content="flex-start" valign_content="flex-start" content_wrap="wrap" spacing="" center_content="no" column_tag="div" link="" target="_self" link_description="" min_height="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" type_medium="" type_small="" order_medium="0" order_small="0" dimension_spacing_medium="" dimension_spacing_small="" dimension_spacing="" dimension_margin_medium="" dimension_margin_small="" margin_top="" margin_bottom="" padding_medium="" padding_small="" padding_top="" padding_right="" padding_bottom="" padding_left="" hover_type="none" border_sizes="" border_color="" border_style="solid" border_radius="" box_shadow="no" dimension_box_shadow="" box_shadow_blur="0" box_shadow_spread="0" box_shadow_color="" box_shadow_style="" z_index_subgroup="regular" z_index="" z_index_hover="" overflow="" background_type="single" gradient_start_color="" gradient_end_color="" gradient_start_position="0" gradient_end_position="100" gradient_type="linear" radial_direction="center center" linear_angle="180" background_color="" background_image="" background_image_id="" lazy_load="none" skip_lazy_load="" background_position="left top" background_repeat="no-repeat" background_blend_mode="none" render_logics="" sticky="off" sticky_devices="small-visibility,medium-visibility,large-visibility" sticky_offset="" absolute="off" absolute_props="" filter_type="regular" filter_hover_element="self" filter_hue="0" filter_saturation="100" filter_brightness="100" filter_contrast="100" filter_invert="0" filter_sepia="0" filter_opacity="100" filter_blur="0" filter_hue_hover="0" filter_saturation_hover="100" filter_brightness_hover="100" filter_contrast_hover="100" filter_invert_hover="0" filter_sepia_hover="0" 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hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

McCoy’s 5A Modified Media (M5AMM)

Background

McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the and the full control you need for mammalian cell culture. MCCOY’S 5A Contains:

High glucose (3g/L)
Non-Essential Amino Acids
Phenol Red
L-glutamine

Why PurMa Biologics? Why we stand behind our cell culture media:

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.

Application

McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas Jurkat cells. Moreover, PurMa^TM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation

complete formulation is available in formulation tab as well as here:McCoy's 5A Formulation

References

Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399

[/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]

Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

McCoy’s 5A Modified Media MAX (M5AMM-MAX)

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McCoy’s 5A Modified Media-MAX (M5AMM-MAX)

Background

McCoy’s 5A Modified Media MAX (M5AMM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of McCoy’s 5A MAX over McCoy’s 5A are:

Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible. Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle. In addition to our Max formulation products, we manufacture PurMa^TMGluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Formulation

The complete formulation can be found here: McCoy's 5A MAX Formulation

References

Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058

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Parameter	Specification
Appearance	Red, clear liquid
pH	7.2 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Additive	Sodium pyruvate
Indicator	Phenol red
Mycoplasma Detection	Negative
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Room temperature

[/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Basal Medium Eagle-MAX (BME-MAX) in Earle’s Buffer

Background

Advantages

The advantages of BME-MAX over BME are:

Mechanism

Contents