Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. EMEM-MAX significantly improves cell viability and growth. We manufacture several types of EMEM-MAX based on your requirements.

Description

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)

Background

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of EMEM-MAX over EMEM are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of EMEM based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

EMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: EMEM-MAX Formulation 

References

  1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, et al. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
  2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, et al. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
  3. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, et al. Histochem Cytobiol. 1988;26(3):123-31. PMID:
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

EMEM MAX Standard Formulation, EMEM MAX w/o Phenol Red, EMEM MAX w/o Sodium Bicarbonate, EMEM MAX w/o Sodium Pyruvate, EMEM MAX w/o Glucose, EMEM MAX w/o Phenol Red, w/o Sodium Bicarbonate, EMEM MAX w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM MAX w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM MAX High Glucose (4.5 g/L), EMEM MAX High Sodium Bicarbonate (2.438 g/L), EMEM MAX High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX 15 mM HEPES (3.6 g/L), EMEM MAX 25 mM HEPES (5.9 g/L), EMEM MAX 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, w/o Phenol Red, EMEM MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), EMEM MAX w/o Phenol Red, with 25 mM HEPES (5.9 g/L), EMEM MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), EMEM MAX w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), EMEM MAX w/o Phenol Red, High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L) High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), EMEM MAX w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), EMEM MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM MAX w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (2.438 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

EMEM MAX Formulation

SDS

SDS

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